CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS
The aim of this study was to assess CXCR4 expression in different subsets of CTCs and single (detached) breast cancer cells.Materials and methods. Thirty five patients with invasive breast carcinoma of no specialtype (IC NST) (T1-4N0-2M0), between 29 and 69 years of age were included in this study....
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2018-09-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/819 |
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| author | O. E. Savelieva L. A. Tashireva M. A. Buldakov R. H. Mukhamedzhanov E. V. Kaigorodova E. V. Denisov M. V. Zavyalova V. M. Perelmuter |
| author_facet | O. E. Savelieva L. A. Tashireva M. A. Buldakov R. H. Mukhamedzhanov E. V. Kaigorodova E. V. Denisov M. V. Zavyalova V. M. Perelmuter |
| author_sort | O. E. Savelieva |
| collection | DOAJ |
| description | The aim of this study was to assess CXCR4 expression in different subsets of CTCs and single (detached) breast cancer cells.Materials and methods. Thirty five patients with invasive breast carcinoma of no specialtype (IC NST) (T1-4N0-2M0), between 29 and 69 years of age were included in this study. Different subsets of CTCs with CXCR4 expression were evaluated by flow cytometry. A confocal microscopy was used to assess CXCR4 expression in different subsets of single (detached) cancer cells in breast tissue.Results. The CXCR4 was expressed in CTCs without stem-like and EMT phenotype, in CTCs with EMT but not stem markers and in stem-like CTCs without EMT features. In all blood samples, the CXCR4 expression in CTCs with stem-like and EMT phenotype was absent. In breast tumor the CXCR4 was expressed in the non stemlike single (detached) breast cancer cells with EMT features, in the single (detached) breast cancer cells with stem and EMT features. In all tumor samples the stem-like or non stem-like single (detached) breast cancer cells without EMT features were absent.Conclusions. Different subsets of the CTCs exhibited CXCR4. The CXCR4 expression did not depend on the presence or absence of stem or/and EMT features in tumor cells. We showed that some subsets of single (detached) breast cancer cells in the primary tumor were characterized by the ability to express CXCR4 and may be a source of the respective CTC subsets. |
| format | Article |
| id | doaj-art-c60039fb012f4002b7f57d5bfb934b03 |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2018-09-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-c60039fb012f4002b7f57d5bfb934b032025-08-20T03:35:48ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682018-09-01174758010.21294/1814-4861-2018-17-4-75-80565CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLSO. E. Savelieva0L. A. Tashireva1M. A. Buldakov2R. H. Mukhamedzhanov3E. V. Kaigorodova4E. V. Denisov5M. V. Zavyalova6V. M. Perelmuter7Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Tomsk State UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Tomsk State UniversitySiberian State Medical UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Siberian State Medical UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Tomsk State UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Tomsk State University Siberian State Medical UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesThe aim of this study was to assess CXCR4 expression in different subsets of CTCs and single (detached) breast cancer cells.Materials and methods. Thirty five patients with invasive breast carcinoma of no specialtype (IC NST) (T1-4N0-2M0), between 29 and 69 years of age were included in this study. Different subsets of CTCs with CXCR4 expression were evaluated by flow cytometry. A confocal microscopy was used to assess CXCR4 expression in different subsets of single (detached) cancer cells in breast tissue.Results. The CXCR4 was expressed in CTCs without stem-like and EMT phenotype, in CTCs with EMT but not stem markers and in stem-like CTCs without EMT features. In all blood samples, the CXCR4 expression in CTCs with stem-like and EMT phenotype was absent. In breast tumor the CXCR4 was expressed in the non stemlike single (detached) breast cancer cells with EMT features, in the single (detached) breast cancer cells with stem and EMT features. In all tumor samples the stem-like or non stem-like single (detached) breast cancer cells without EMT features were absent.Conclusions. Different subsets of the CTCs exhibited CXCR4. The CXCR4 expression did not depend on the presence or absence of stem or/and EMT features in tumor cells. We showed that some subsets of single (detached) breast cancer cells in the primary tumor were characterized by the ability to express CXCR4 and may be a source of the respective CTC subsets.https://www.siboncoj.ru/jour/article/view/819cxcr4circulating tumor cellscancer stem-like cellssingle (detached) cancer cellsepithelial-mesenchymal transitionbreast cancer |
| spellingShingle | O. E. Savelieva L. A. Tashireva M. A. Buldakov R. H. Mukhamedzhanov E. V. Kaigorodova E. V. Denisov M. V. Zavyalova V. M. Perelmuter CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS Сибирский онкологический журнал cxcr4 circulating tumor cells cancer stem-like cells single (detached) cancer cells epithelial-mesenchymal transition breast cancer |
| title | CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS |
| title_full | CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS |
| title_fullStr | CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS |
| title_full_unstemmed | CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS |
| title_short | CXCR4 EXPRESSION IN DIFFERENT SUBSETS OF CTCs AND SINGLE (DETACHED) BREAST CANCER CELLS |
| title_sort | cxcr4 expression in different subsets of ctcs and single detached breast cancer cells |
| topic | cxcr4 circulating tumor cells cancer stem-like cells single (detached) cancer cells epithelial-mesenchymal transition breast cancer |
| url | https://www.siboncoj.ru/jour/article/view/819 |
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