Design and evaluation of a multi-epitope DNA vaccine against HPV16
Cervical cancer, among the deadliest cancers affecting women globally, primarily arises from persistent infection with high-risk human papillomavirus (HPV). To effectively combat persistent infection and prevent the progression of precancerous lesions into malignancy, a therapeutic HPV vaccine is un...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21645515.2024.2352908 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850185593863012352 |
|---|---|
| author | Lanfang Zhu Xiangjie Cui Zhiling Yan Yufen Tao Lei Shi Xinwen Zhang Yufeng Yao Li Shi |
| author_facet | Lanfang Zhu Xiangjie Cui Zhiling Yan Yufen Tao Lei Shi Xinwen Zhang Yufeng Yao Li Shi |
| author_sort | Lanfang Zhu |
| collection | DOAJ |
| description | Cervical cancer, among the deadliest cancers affecting women globally, primarily arises from persistent infection with high-risk human papillomavirus (HPV). To effectively combat persistent infection and prevent the progression of precancerous lesions into malignancy, a therapeutic HPV vaccine is under development. This study utilized an immunoinformatics approach to predict epitopes of cytotoxic T lymphocytes (CTLs) and helper T lymphocytes (HTLs) using the E6 and E7 oncoproteins of the HPV16 strain as target antigens. Subsequently, through meticulous selection of T-cell epitopes and other necessary elements, a multi-epitope vaccine was constructed, exhibiting good immunogenic, physicochemical, and structural characteristics. Furthermore, in silico simulations showed that the vaccine not only interacted well with toll-like receptors (TLR2/TLR3/TLR4), but also induced a strong innate and adaptive immune response characterized by elevated Th1-type cytokines, such as interferon-gamma (IFN-γ) and interleukin-2 (IL2). Additionally, our study investigated the effects of different immunization intervals on immune responses, aiming to optimize a time-efficient immunization program. In animal model experiments, the vaccine exhibited robust immunogenic, therapeutic, and prophylactic effects. Administered thrice, it consistently induced the expansion of specific CD4 and CD8 T cells, resulting in substantial cytokines release and increased proliferation of memory T cell subsets in splenic cells. Overall, our findings support the potential of this multi-epitope vaccine in combating HPV16 infection and signify its candidacy for future HPV vaccine development. |
| format | Article |
| id | doaj-art-c5fcac59691546ddb006a8f86a2ad1b8 |
| institution | OA Journals |
| issn | 2164-5515 2164-554X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj-art-c5fcac59691546ddb006a8f86a2ad1b82025-08-20T02:16:40ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2024-12-0120110.1080/21645515.2024.2352908Design and evaluation of a multi-epitope DNA vaccine against HPV16Lanfang Zhu0Xiangjie Cui1Zhiling Yan2Yufen Tao3Lei Shi4Xinwen Zhang5Yufeng Yao6Li Shi7Department of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaDepartment of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaDepartment of Gynaecologic Oncology, The No. 3 Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaDepartment of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaDepartment of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaYunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaDepartment of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, ChinaCervical cancer, among the deadliest cancers affecting women globally, primarily arises from persistent infection with high-risk human papillomavirus (HPV). To effectively combat persistent infection and prevent the progression of precancerous lesions into malignancy, a therapeutic HPV vaccine is under development. This study utilized an immunoinformatics approach to predict epitopes of cytotoxic T lymphocytes (CTLs) and helper T lymphocytes (HTLs) using the E6 and E7 oncoproteins of the HPV16 strain as target antigens. Subsequently, through meticulous selection of T-cell epitopes and other necessary elements, a multi-epitope vaccine was constructed, exhibiting good immunogenic, physicochemical, and structural characteristics. Furthermore, in silico simulations showed that the vaccine not only interacted well with toll-like receptors (TLR2/TLR3/TLR4), but also induced a strong innate and adaptive immune response characterized by elevated Th1-type cytokines, such as interferon-gamma (IFN-γ) and interleukin-2 (IL2). Additionally, our study investigated the effects of different immunization intervals on immune responses, aiming to optimize a time-efficient immunization program. In animal model experiments, the vaccine exhibited robust immunogenic, therapeutic, and prophylactic effects. Administered thrice, it consistently induced the expansion of specific CD4 and CD8 T cells, resulting in substantial cytokines release and increased proliferation of memory T cell subsets in splenic cells. Overall, our findings support the potential of this multi-epitope vaccine in combating HPV16 infection and signify its candidacy for future HPV vaccine development.https://www.tandfonline.com/doi/10.1080/21645515.2024.2352908HPVmulti-epitope vaccinecancer vaccineimmunoinformaticsimmune simulation |
| spellingShingle | Lanfang Zhu Xiangjie Cui Zhiling Yan Yufen Tao Lei Shi Xinwen Zhang Yufeng Yao Li Shi Design and evaluation of a multi-epitope DNA vaccine against HPV16 Human Vaccines & Immunotherapeutics HPV multi-epitope vaccine cancer vaccine immunoinformatics immune simulation |
| title | Design and evaluation of a multi-epitope DNA vaccine against HPV16 |
| title_full | Design and evaluation of a multi-epitope DNA vaccine against HPV16 |
| title_fullStr | Design and evaluation of a multi-epitope DNA vaccine against HPV16 |
| title_full_unstemmed | Design and evaluation of a multi-epitope DNA vaccine against HPV16 |
| title_short | Design and evaluation of a multi-epitope DNA vaccine against HPV16 |
| title_sort | design and evaluation of a multi epitope dna vaccine against hpv16 |
| topic | HPV multi-epitope vaccine cancer vaccine immunoinformatics immune simulation |
| url | https://www.tandfonline.com/doi/10.1080/21645515.2024.2352908 |
| work_keys_str_mv | AT lanfangzhu designandevaluationofamultiepitopednavaccineagainsthpv16 AT xiangjiecui designandevaluationofamultiepitopednavaccineagainsthpv16 AT zhilingyan designandevaluationofamultiepitopednavaccineagainsthpv16 AT yufentao designandevaluationofamultiepitopednavaccineagainsthpv16 AT leishi designandevaluationofamultiepitopednavaccineagainsthpv16 AT xinwenzhang designandevaluationofamultiepitopednavaccineagainsthpv16 AT yufengyao designandevaluationofamultiepitopednavaccineagainsthpv16 AT lishi designandevaluationofamultiepitopednavaccineagainsthpv16 |