Development of a Novel Automated Workflow in Fiji ImageJ for Batch Analysis of Confocal Imaging Data to Quantify Protein Colocalization Using Manders Coefficient
Confocal microscopy is integral to molecular and cellular biology, enabling high-resolution imaging and colocalization studies to elucidate biomolecular interactions in cells. Despite its utility, challenges in handling large datasets, particularly in preprocessing Z-stacks and calculating colocaliz...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-04-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5285&type=0 |
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