Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway
Introduction In this study, we investigated the clinical value of using urothelial cancer-associated 1 (UCA1) and microRNA-16 (miR-16) as biomarkers for the diagnosis of pre-eclampsia (PE). Also, we compared the diagnostic values of miR-16, UCA1 and pregnancy-associated plasma protein-A (PAPP-A) in...
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Termedia Publishing House
2021-03-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/Identification-of-urothelial-cancer-associated-1-UCA1-as-a-diagnostic-biomarker-of,111375,0,2.html |
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| author | Ye-Hong Xv Wei Gao Liao-Jv Wang |
| author_facet | Ye-Hong Xv Wei Gao Liao-Jv Wang |
| author_sort | Ye-Hong Xv |
| collection | DOAJ |
| description | Introduction
In this study, we investigated the clinical value of using urothelial cancer-associated 1 (UCA1) and microRNA-16 (miR-16) as biomarkers for the diagnosis of pre-eclampsia (PE). Also, we compared the diagnostic values of miR-16, UCA1 and pregnancy-associated plasma protein-A (PAPP-A) in PE. Furthermore, we investigated the interaction between miR-16 and UCA1/PAPP-A.
Material and methods
128 PE patients and 172 healthy pregnant women were enrolled in this study. Receiver operating characteristic (ROC) analysis was carried out to predict the diagnostic values of UCA1, miR-16 and PAPP-A in PE. Enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (PCR), Western blot analysis, immunohistochemistry (IHC) assay, computational analysis, and luciferase assay were conducted to measure the differential expression of UCA1, miR-16, and PAPP A while establishing a signaling pathway of UCA1/miR-16/PAPP-A.
Results
Compared with miR-16 and PAPP-A, UCA1 exhibited a better value in the diagnosis of PE. The expression of PAPP-A and UCA1 was down-regulated while the expression of miR-16 was up-regulated in patients with PE, especially in patients with HELLP pregnancies. Moreover, UCA1 was identified as a sponge of miR-16, while PAPP-A mRNA was identified as a virtual target gene of miR-16. Finally, a negative regulatory relationship was observed between the expression of miR-16 and UCA1 or PAPP-A, while the expression of UCA1 and PAPP-A were positively related.
Conclusions
Taken together, the evidence suggests that UCA1 could be used as a more valuable biomarker in the diagnosis of PE. Meanwhile, the reduced expression of UCA1 could exert a positive effect by reducing the expression of PAPP-A in the pathogenesis of PE. |
| format | Article |
| id | doaj-art-c5efa4bba077454f804cc2d712df2814 |
| institution | Kabale University |
| issn | 1734-1922 1896-9151 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj-art-c5efa4bba077454f804cc2d712df28142025-01-27T10:44:31ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512021-03-012051511152110.5114/aoms/111375111375Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathwayYe-Hong Xv0Wei Gao1Liao-Jv Wang2Department of Obstetrics, Northwest Women’s and Children’s Hospital, Xi’an, Shaanxi, ChinaDepartment of Obstetrics, The First People’s Hospital of Xianyang City, Xianyang, Shaanxi, ChinaDepartment of Obstetrics, The First People’s Hospital of Xianyang City, Xianyang, Shaanxi, ChinaIntroduction In this study, we investigated the clinical value of using urothelial cancer-associated 1 (UCA1) and microRNA-16 (miR-16) as biomarkers for the diagnosis of pre-eclampsia (PE). Also, we compared the diagnostic values of miR-16, UCA1 and pregnancy-associated plasma protein-A (PAPP-A) in PE. Furthermore, we investigated the interaction between miR-16 and UCA1/PAPP-A. Material and methods 128 PE patients and 172 healthy pregnant women were enrolled in this study. Receiver operating characteristic (ROC) analysis was carried out to predict the diagnostic values of UCA1, miR-16 and PAPP-A in PE. Enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (PCR), Western blot analysis, immunohistochemistry (IHC) assay, computational analysis, and luciferase assay were conducted to measure the differential expression of UCA1, miR-16, and PAPP A while establishing a signaling pathway of UCA1/miR-16/PAPP-A. Results Compared with miR-16 and PAPP-A, UCA1 exhibited a better value in the diagnosis of PE. The expression of PAPP-A and UCA1 was down-regulated while the expression of miR-16 was up-regulated in patients with PE, especially in patients with HELLP pregnancies. Moreover, UCA1 was identified as a sponge of miR-16, while PAPP-A mRNA was identified as a virtual target gene of miR-16. Finally, a negative regulatory relationship was observed between the expression of miR-16 and UCA1 or PAPP-A, while the expression of UCA1 and PAPP-A were positively related. Conclusions Taken together, the evidence suggests that UCA1 could be used as a more valuable biomarker in the diagnosis of PE. Meanwhile, the reduced expression of UCA1 could exert a positive effect by reducing the expression of PAPP-A in the pathogenesis of PE.https://www.archivesofmedicalscience.com/Identification-of-urothelial-cancer-associated-1-UCA1-as-a-diagnostic-biomarker-of,111375,0,2.htmlpre-eclampsiaurothelial cancer-associated 1microrna-16pregnancy-associated plasma protein-a |
| spellingShingle | Ye-Hong Xv Wei Gao Liao-Jv Wang Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway Archives of Medical Science pre-eclampsia urothelial cancer-associated 1 microrna-16 pregnancy-associated plasma protein-a |
| title | Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway |
| title_full | Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway |
| title_fullStr | Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway |
| title_full_unstemmed | Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway |
| title_short | Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway |
| title_sort | identification of urothelial cancer associated 1 uca1 as a diagnostic biomarker of pre eclampsia via regulating microrna 16 and its downstream signaling pathway |
| topic | pre-eclampsia urothelial cancer-associated 1 microrna-16 pregnancy-associated plasma protein-a |
| url | https://www.archivesofmedicalscience.com/Identification-of-urothelial-cancer-associated-1-UCA1-as-a-diagnostic-biomarker-of,111375,0,2.html |
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