Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E
Three different centrifugation frequencies viz., zero time (ZTC), one time (ITW), two times (2TC) and three times (3TC) supplemented with different levels of vitamin E were studied under in vitro cryosurvival of West African Dwarf (WAD) goat spermatozoa. Semen samples were washed ITW, 2TC and 3TC an...
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AcademicPres
2024-11-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies |
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| Online Access: | https://journals.usamvcluj.ro/index.php/zootehnie/article/view/14951 |
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| author | Ezekiel ADEKUNLE Adebisi ODEYEMI Bukola MAJEKODUNMI Oluwaseun AKOSILE Obafemi AKINJUTE James DARAMOLA Okanlawon ONAGBESAN Olusiji SOWANDE |
| author_facet | Ezekiel ADEKUNLE Adebisi ODEYEMI Bukola MAJEKODUNMI Oluwaseun AKOSILE Obafemi AKINJUTE James DARAMOLA Okanlawon ONAGBESAN Olusiji SOWANDE |
| author_sort | Ezekiel ADEKUNLE |
| collection | DOAJ |
| description | Three different centrifugation frequencies viz., zero time (ZTC), one time (ITW), two times (2TC) and three times (3TC) supplemented with different levels of vitamin E were studied under in vitro cryosurvival of West African Dwarf (WAD) goat spermatozoa. Semen samples were washed ITW, 2TC and 3TC and supplemented each with 2, 4, 6 and 8 mM of vitamin E in a 3x4 factorial arrangements and cryopreserved for 30 days in liquid nitrogen. Data were subjected to one-way ANOVA. Spermatozoa cryopreserved with Tris-based extender supplemented with 6 and 8 mM using 3TC and 8 mM with 2TC had highest (P<0.05) percentage motility, percentage acrosome integrity and percentage live spermatozoa while highest (P<0.05) percentage membrane integrity were observed at 4, 6 and 8 mM. Cryopreserved spermatozoa with 6 and 8 mM for 3TC had the lowest (P<0.05) percentage abnormality and highest (P<0.05) percentage spermatozoa that underwent acrosome reaction including 8 mM using 2TC. Also 4, 6 and 8 mM for 3TC and 6 and 8 mM using 2TC had the highest (P<0.05) percentage spermatozoa that underwent capacitation. The results showed lowest (P < 0.05) concentrations of Malondialdehyde MDA at 2, 4, 6 and 8 mM for 1TC, 6 and 8 mM for 2TC and ZTC; and 8 mM vitamin E for 3TC. However, the Tris extenders supplemented with 2 and 4 mM for ZTC and 4 mM for 1TC had highest (P<0.05) values for arginase activity. Cryopreservation of WAD goat buck sperm with 2TC and 3TC improved viability and fertilizing ability. |
| format | Article |
| id | doaj-art-c5e586f9304a4d589ee42587eebf6c3b |
| institution | OA Journals |
| issn | 1843-5262 1843-536X |
| language | English |
| publishDate | 2024-11-01 |
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| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies |
| spelling | doaj-art-c5e586f9304a4d589ee42587eebf6c3b2025-08-20T02:07:12ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies1843-52621843-536X2024-11-01812102010.15835/buasvmcn-asb:2024.001014951Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin EEzekiel ADEKUNLE0Adebisi ODEYEMI1Bukola MAJEKODUNMI2Oluwaseun AKOSILE3Obafemi AKINJUTE4James DARAMOLA5Okanlawon ONAGBESAN6Olusiji SOWANDE7Department of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Physiology, Federal University of Agriculture Abeokuta, NigeriaDepartment of Animal Production & Health, Federal University of Agriculture Abeokuta, NigeriaThree different centrifugation frequencies viz., zero time (ZTC), one time (ITW), two times (2TC) and three times (3TC) supplemented with different levels of vitamin E were studied under in vitro cryosurvival of West African Dwarf (WAD) goat spermatozoa. Semen samples were washed ITW, 2TC and 3TC and supplemented each with 2, 4, 6 and 8 mM of vitamin E in a 3x4 factorial arrangements and cryopreserved for 30 days in liquid nitrogen. Data were subjected to one-way ANOVA. Spermatozoa cryopreserved with Tris-based extender supplemented with 6 and 8 mM using 3TC and 8 mM with 2TC had highest (P<0.05) percentage motility, percentage acrosome integrity and percentage live spermatozoa while highest (P<0.05) percentage membrane integrity were observed at 4, 6 and 8 mM. Cryopreserved spermatozoa with 6 and 8 mM for 3TC had the lowest (P<0.05) percentage abnormality and highest (P<0.05) percentage spermatozoa that underwent acrosome reaction including 8 mM using 2TC. Also 4, 6 and 8 mM for 3TC and 6 and 8 mM using 2TC had the highest (P<0.05) percentage spermatozoa that underwent capacitation. The results showed lowest (P < 0.05) concentrations of Malondialdehyde MDA at 2, 4, 6 and 8 mM for 1TC, 6 and 8 mM for 2TC and ZTC; and 8 mM vitamin E for 3TC. However, the Tris extenders supplemented with 2 and 4 mM for ZTC and 4 mM for 1TC had highest (P<0.05) values for arginase activity. Cryopreservation of WAD goat buck sperm with 2TC and 3TC improved viability and fertilizing ability.https://journals.usamvcluj.ro/index.php/zootehnie/article/view/14951cryopreservation; wad buck; seminal plasma; fertilizing ability; sperm viability. |
| spellingShingle | Ezekiel ADEKUNLE Adebisi ODEYEMI Bukola MAJEKODUNMI Oluwaseun AKOSILE Obafemi AKINJUTE James DARAMOLA Okanlawon ONAGBESAN Olusiji SOWANDE Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies cryopreservation; wad buck; seminal plasma; fertilizing ability; sperm viability. |
| title | Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E |
| title_full | Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E |
| title_fullStr | Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E |
| title_full_unstemmed | Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E |
| title_short | Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E |
| title_sort | effect of three different centrifugation on cryosurvival of goat spermatozoa supplemented with vitamin e |
| topic | cryopreservation; wad buck; seminal plasma; fertilizing ability; sperm viability. |
| url | https://journals.usamvcluj.ro/index.php/zootehnie/article/view/14951 |
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