Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract
Objective(s): Natural component-included scaffolds can provide numerous benefits for skin healing and tissue regeneration. Nanofibers (NFs) with intricately intertwined three-dimensional structures afford an exclusive matrix for delivering therapeutics. This research assessed nanofibrous scaffolds l...
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Mashhad University of Medical Sciences
2025-01-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_25178_71c1013634357844e4ebcdc5fce84c3a.pdf |
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| author | Omid Tavallaei Mahtab Doostan Kamyar Khoshnevisan Ali Tahmasebi Hassan Maleki |
| author_facet | Omid Tavallaei Mahtab Doostan Kamyar Khoshnevisan Ali Tahmasebi Hassan Maleki |
| author_sort | Omid Tavallaei |
| collection | DOAJ |
| description | Objective(s): Natural component-included scaffolds can provide numerous benefits for skin healing and tissue regeneration. Nanofibers (NFs) with intricately intertwined three-dimensional structures afford an exclusive matrix for delivering therapeutics. This research assessed nanofibrous scaffolds loaded with Achillea wilhelmsii extract (5-15 wt%) for skin tissue engineering. Materials and Methods: A. wilhelmsii-loaded scaffolds, composed of poly(vinyl alcohol) (PVA) and chitosan (CS), were fabricated by the electrospinning process. Subsequently, the physicochemical properties of the scaffolds were evaluated through relevant analyses. The antioxidant activity and degradation rate of the scaffolds were also determined. Cell viability and scratch assays on dermal fibroblasts were conducted to assess proliferation and migration activities. Results: Electron micrographs revealed interconnected fibers with a nano-scale diameter (> 400 nm) and uniform morphology. Additionally, the intact presence of A. wilhelmsii extract in the polymeric matrix was confirmed without any undesirable interactions. The proposed scaffolds verified favorable mechanical properties, a hydrophilic nature, high volume porosity (>90%), and water absorption capability (<500%). Besides, the findings demonstrated the remarkable radical scavenging ability of A. wilhelmsii extract in the nanofibrous scaffolds, along with controlled degradation kinetics over 72 h. The viability assay proved that the A. wilhelmsii-loaded scaffolds not only exhibited no cytotoxicity but also improved cell proliferation. The scaffolds also significantly accelerated fibroblast migration and complete closure of scratched areas. Conclusion: At last, the obtained results revealed that A. wilhelmsii-loaded PVA/CS NFs can be applied as a potential scaffold for skin regeneration and wound healing promotion. |
| format | Article |
| id | doaj-art-c5e52deb700f4d6ca32cf7c07f4a48de |
| institution | DOAJ |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-c5e52deb700f4d6ca32cf7c07f4a48de2025-08-20T03:09:12ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-01-0112111012110.22038/nmj.2024.79495.196325178Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extractOmid Tavallaei0Mahtab Doostan1Kamyar Khoshnevisan2Ali Tahmasebi3Hassan Maleki4Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IranStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranMedical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 1983963113, IranStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IranObjective(s): Natural component-included scaffolds can provide numerous benefits for skin healing and tissue regeneration. Nanofibers (NFs) with intricately intertwined three-dimensional structures afford an exclusive matrix for delivering therapeutics. This research assessed nanofibrous scaffolds loaded with Achillea wilhelmsii extract (5-15 wt%) for skin tissue engineering. Materials and Methods: A. wilhelmsii-loaded scaffolds, composed of poly(vinyl alcohol) (PVA) and chitosan (CS), were fabricated by the electrospinning process. Subsequently, the physicochemical properties of the scaffolds were evaluated through relevant analyses. The antioxidant activity and degradation rate of the scaffolds were also determined. Cell viability and scratch assays on dermal fibroblasts were conducted to assess proliferation and migration activities. Results: Electron micrographs revealed interconnected fibers with a nano-scale diameter (> 400 nm) and uniform morphology. Additionally, the intact presence of A. wilhelmsii extract in the polymeric matrix was confirmed without any undesirable interactions. The proposed scaffolds verified favorable mechanical properties, a hydrophilic nature, high volume porosity (>90%), and water absorption capability (<500%). Besides, the findings demonstrated the remarkable radical scavenging ability of A. wilhelmsii extract in the nanofibrous scaffolds, along with controlled degradation kinetics over 72 h. The viability assay proved that the A. wilhelmsii-loaded scaffolds not only exhibited no cytotoxicity but also improved cell proliferation. The scaffolds also significantly accelerated fibroblast migration and complete closure of scratched areas. Conclusion: At last, the obtained results revealed that A. wilhelmsii-loaded PVA/CS NFs can be applied as a potential scaffold for skin regeneration and wound healing promotion.https://nmj.mums.ac.ir/article_25178_71c1013634357844e4ebcdc5fce84c3a.pdfantioxidantselectrospinningnanofiberstissue scaffoldswound healing |
| spellingShingle | Omid Tavallaei Mahtab Doostan Kamyar Khoshnevisan Ali Tahmasebi Hassan Maleki Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract Nanomedicine Journal antioxidants electrospinning nanofibers tissue scaffolds wound healing |
| title | Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract |
| title_full | Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract |
| title_fullStr | Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract |
| title_full_unstemmed | Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract |
| title_short | Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract |
| title_sort | wound healing promotion by poly vinyl alcohol chitosan electrospun nanofibrous scaffold loaded with achillea wilhelmsii extract |
| topic | antioxidants electrospinning nanofibers tissue scaffolds wound healing |
| url | https://nmj.mums.ac.ir/article_25178_71c1013634357844e4ebcdc5fce84c3a.pdf |
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