Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico

With the first evidence of the association between bats and influenza A viruses, various studies have begun to emerge to understand this interesting and important association among bats conservation, animal health, and public health. This study aimed to verify the presence of anti-influenza A vipoth...

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Main Authors: Brenda Aline Maya-Badillo, Guillermo Orta-Pineda, Gerardo Suzán, Karen Elizabeth Rivera-Rosas, Diego Zavala-Vasco, Adrián Uribe-Jacinto, Andrea Chaves, Alfredo Grande-Cano, René Segura-Velazquez, José Iván Sánchez-Betancourt
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Pathogens
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Online Access:https://www.mdpi.com/2076-0817/14/5/414
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author Brenda Aline Maya-Badillo
Guillermo Orta-Pineda
Gerardo Suzán
Karen Elizabeth Rivera-Rosas
Diego Zavala-Vasco
Adrián Uribe-Jacinto
Andrea Chaves
Alfredo Grande-Cano
René Segura-Velazquez
José Iván Sánchez-Betancourt
author_facet Brenda Aline Maya-Badillo
Guillermo Orta-Pineda
Gerardo Suzán
Karen Elizabeth Rivera-Rosas
Diego Zavala-Vasco
Adrián Uribe-Jacinto
Andrea Chaves
Alfredo Grande-Cano
René Segura-Velazquez
José Iván Sánchez-Betancourt
author_sort Brenda Aline Maya-Badillo
collection DOAJ
description With the first evidence of the association between bats and influenza A viruses, various studies have begun to emerge to understand this interesting and important association among bats conservation, animal health, and public health. This study aimed to verify the presence of anti-influenza A vipothesrus antibodies, as well as the molecular identification of these viruses in bats distributed in forest fragments located in southeastern Mexico. Blood samples were obtained from 600 bats belonging to 24 different species, using an enzyme immunoassay to detect antibodies against the nucleoprotein antigen of the avian influenza A virus. Likewise, oropharyngeal swabs, rectal swabs and organs were taken for quantitative reverse transcription PCR (qRT-PCR) of these viruses. A total of six bats (1%) tested positive either by serology or molecular methods, not both simultaneously. Although this suggests a very low prevalence of influenza A viruses in Mexican bats, it is the first study to address this association and, following the precautionary principle, we consider it necessary to establish systematic monitoring of the presence of influenza A in bats, since they are known to harbor infectious agents with zoonotic potential. Furthermore, it is possible that the association of influenza A viruses circulating in Latin American bats has an important co-evolutionary component with some bat species with exclusive distribution in the American continent.
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spelling doaj-art-c5db2ff27eba4feb9738059ce7a4f46c2025-08-20T02:33:55ZengMDPI AGPathogens2076-08172025-04-0114541410.3390/pathogens14050414Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from MexicoBrenda Aline Maya-Badillo0Guillermo Orta-Pineda1Gerardo Suzán2Karen Elizabeth Rivera-Rosas3Diego Zavala-Vasco4Adrián Uribe-Jacinto5Andrea Chaves6Alfredo Grande-Cano7René Segura-Velazquez8José Iván Sánchez-Betancourt9Laboratorio de Ecología de Enfermedades y Una Salud del Departamento de Etología, Fauna Silvestre y Animales de Laboratorio, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoLaboratorio de Ecología de Enfermedades y Una Salud del Departamento de Etología, Fauna Silvestre y Animales de Laboratorio, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoLaboratorio de Ecología de Enfermedades y Una Salud del Departamento de Etología, Fauna Silvestre y Animales de Laboratorio, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoLaboratorio de Investigación del Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoLaboratorio de Investigación del Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoDepartmento de Ciencias Agropecuariast, Universidad Juárez Autónoma de Tabasco, Villahermosa 86025, MexicoCentro Nacional de Innovaciones Biotecnológicas (CENIBiot), CeNAT, Conare, San José 1174-1200, Costa RicaDepartmento de Secuenciación, Unidad Universitaria de Secuenciación Masiva de DNA-UNAM, Cuernavaca 62210, MexicoUnidad de Investigación de la Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de Mexico 04510, MexicoLaboratorio de Investigación del Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoWith the first evidence of the association between bats and influenza A viruses, various studies have begun to emerge to understand this interesting and important association among bats conservation, animal health, and public health. This study aimed to verify the presence of anti-influenza A vipothesrus antibodies, as well as the molecular identification of these viruses in bats distributed in forest fragments located in southeastern Mexico. Blood samples were obtained from 600 bats belonging to 24 different species, using an enzyme immunoassay to detect antibodies against the nucleoprotein antigen of the avian influenza A virus. Likewise, oropharyngeal swabs, rectal swabs and organs were taken for quantitative reverse transcription PCR (qRT-PCR) of these viruses. A total of six bats (1%) tested positive either by serology or molecular methods, not both simultaneously. Although this suggests a very low prevalence of influenza A viruses in Mexican bats, it is the first study to address this association and, following the precautionary principle, we consider it necessary to establish systematic monitoring of the presence of influenza A in bats, since they are known to harbor infectious agents with zoonotic potential. Furthermore, it is possible that the association of influenza A viruses circulating in Latin American bats has an important co-evolutionary component with some bat species with exclusive distribution in the American continent.https://www.mdpi.com/2076-0817/14/5/414<i>Alphainfluenzavirus</i>forest batsprevalencesoutheast Mexicotropical forests
spellingShingle Brenda Aline Maya-Badillo
Guillermo Orta-Pineda
Gerardo Suzán
Karen Elizabeth Rivera-Rosas
Diego Zavala-Vasco
Adrián Uribe-Jacinto
Andrea Chaves
Alfredo Grande-Cano
René Segura-Velazquez
José Iván Sánchez-Betancourt
Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico
Pathogens
<i>Alphainfluenzavirus</i>
forest bats
prevalence
southeast Mexico
tropical forests
title Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico
title_full Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico
title_fullStr Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico
title_full_unstemmed Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico
title_short Evidence Suggesting Possible Exposure to Influenza A Virus in Neotropical Bats from Mexico
title_sort evidence suggesting possible exposure to influenza a virus in neotropical bats from mexico
topic <i>Alphainfluenzavirus</i>
forest bats
prevalence
southeast Mexico
tropical forests
url https://www.mdpi.com/2076-0817/14/5/414
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