Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia
ABSTRACT Purpose: To characterize histologically and stereologically the hepatic steatosis in obese Zucker (fat, n = 6, with a mutation in the leptin receptor – Leprfa) and control Zucker (lean, n = 6) rats, analyzing macroscopic and microscopic differences to understand the influence of obesity on...
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2025-03-01
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| Series: | Acta Cirúrgica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100212&tlng=en |
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| author | Silvio Pires Gomes Gabriela Salim de Castro Vinicius Pedro Silva de Oliveira Bruno Cogliati Andressa Galvão da Silva Iacopino Ivanir Santana de Oliveira Pires Bruno Cesar Schimming Fernanda Gosuen Gonçalves Dias José Roberto Kfoury Junior Tais Harumi de Castro Sasahara |
| author_facet | Silvio Pires Gomes Gabriela Salim de Castro Vinicius Pedro Silva de Oliveira Bruno Cogliati Andressa Galvão da Silva Iacopino Ivanir Santana de Oliveira Pires Bruno Cesar Schimming Fernanda Gosuen Gonçalves Dias José Roberto Kfoury Junior Tais Harumi de Castro Sasahara |
| author_sort | Silvio Pires Gomes |
| collection | DOAJ |
| description | ABSTRACT Purpose: To characterize histologically and stereologically the hepatic steatosis in obese Zucker (fat, n = 6, with a mutation in the leptin receptor – Leprfa) and control Zucker (lean, n = 6) rats, analyzing macroscopic and microscopic differences to understand the influence of obesity on hepatic pathology. Methods: Zucker rats were fed standard chow for 90 days. Macroscopic, qualitative, and histoquantitative (stereological) approaches were used, involving body and liver weight measurement, morphological analysis, and histopathological classification of metabolic dysfunction-associated steatotic liver disease. Results: Zucker fat rats had higher body weight (p = 0.0022), liver weight (p = 0.0022), serum total cholesterol (p = 0.0022), and triacylglycerol (p = 0.0022) compared to Zucker lean rats. Stereological analysis showed that hepatocyte volume density (p = 0.0022) and total hepatocyte volume (p = 0.0001) were lower, and the volume density (p = 0.002) and total volume of steatosis (p = 0.002) were higher in Zucker fat rats compared to lean rats. Conclusion: The findings indicated that obesity induces significant alterations in the hepatic morphology of Zucker rats, showing that hepatocyte volume is lower in obese animals. This study reinforces the utility of the obese Zucker rat model to investigate the effects of obesity on liver health and suggests hepatic steatosis requires therapeutic strategies focused on modulating these parameters. |
| format | Article |
| id | doaj-art-c5dae7e5d495412d8392883d04ab66f8 |
| institution | DOAJ |
| issn | 1678-2674 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj-art-c5dae7e5d495412d8392883d04ab66f82025-08-20T03:06:46ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742025-03-014010.1590/acb402325Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemiaSilvio Pires Gomeshttps://orcid.org/0000-0001-9981-1230Gabriela Salim de Castrohttps://orcid.org/0000-0002-1758-9857Vinicius Pedro Silva de Oliveirahttps://orcid.org/0000-0003-3663-7575Bruno Cogliatihttps://orcid.org/0000-0002-1388-7240Andressa Galvão da Silva Iacopinohttps://orcid.org/0009-0008-7561-4476Ivanir Santana de Oliveira Pireshttps://orcid.org/0009-0007-3072-6055Bruno Cesar Schimminghttps://orcid.org/0000-0002-0837-9612Fernanda Gosuen Gonçalves Diashttps://orcid.org/0000-0001-6072-4789José Roberto Kfoury Juniorhttps://orcid.org/0000-0002-1284-4038Tais Harumi de Castro Sasaharahttps://orcid.org/0000-0002-4871-5625ABSTRACT Purpose: To characterize histologically and stereologically the hepatic steatosis in obese Zucker (fat, n = 6, with a mutation in the leptin receptor – Leprfa) and control Zucker (lean, n = 6) rats, analyzing macroscopic and microscopic differences to understand the influence of obesity on hepatic pathology. Methods: Zucker rats were fed standard chow for 90 days. Macroscopic, qualitative, and histoquantitative (stereological) approaches were used, involving body and liver weight measurement, morphological analysis, and histopathological classification of metabolic dysfunction-associated steatotic liver disease. Results: Zucker fat rats had higher body weight (p = 0.0022), liver weight (p = 0.0022), serum total cholesterol (p = 0.0022), and triacylglycerol (p = 0.0022) compared to Zucker lean rats. Stereological analysis showed that hepatocyte volume density (p = 0.0022) and total hepatocyte volume (p = 0.0001) were lower, and the volume density (p = 0.002) and total volume of steatosis (p = 0.002) were higher in Zucker fat rats compared to lean rats. Conclusion: The findings indicated that obesity induces significant alterations in the hepatic morphology of Zucker rats, showing that hepatocyte volume is lower in obese animals. This study reinforces the utility of the obese Zucker rat model to investigate the effects of obesity on liver health and suggests hepatic steatosis requires therapeutic strategies focused on modulating these parameters.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100212&tlng=enObesityRats, ZuckerLiverFatty LiverHepatocytes |
| spellingShingle | Silvio Pires Gomes Gabriela Salim de Castro Vinicius Pedro Silva de Oliveira Bruno Cogliati Andressa Galvão da Silva Iacopino Ivanir Santana de Oliveira Pires Bruno Cesar Schimming Fernanda Gosuen Gonçalves Dias José Roberto Kfoury Junior Tais Harumi de Castro Sasahara Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia Acta Cirúrgica Brasileira Obesity Rats, Zucker Liver Fatty Liver Hepatocytes |
| title | Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia |
| title_full | Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia |
| title_fullStr | Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia |
| title_full_unstemmed | Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia |
| title_short | Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia |
| title_sort | hepatic stereological analysis in obese zucker rats leprfa with dyslipidemia |
| topic | Obesity Rats, Zucker Liver Fatty Liver Hepatocytes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100212&tlng=en |
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