Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses
We have created several high protein (HPBL) and low protein breeding lines (LPBL) by crossing between NC-Raleigh and PI407228. The NC-Raleigh is a low protein cultivar widely grown in North Carolina with excellent yield potential and a high protein wild soybean (Glycine soja) with low yield. We anal...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324004927 |
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| author | Nazrul Islam Qijian Song Savithiry Natarajan |
| author_facet | Nazrul Islam Qijian Song Savithiry Natarajan |
| author_sort | Nazrul Islam |
| collection | DOAJ |
| description | We have created several high protein (HPBL) and low protein breeding lines (LPBL) by crossing between NC-Raleigh and PI407228. The NC-Raleigh is a low protein cultivar widely grown in North Carolina with excellent yield potential and a high protein wild soybean (Glycine soja) with low yield. We analyzed the F6 generation of this cross for protein and oil compositions using near-infrared spectroscopy (NIR). Two soybean lines HPBL and LPBL were selected for biochemical analysis using mass spectrometry-based proteomic and metabolomic techniques. We identified 4538 proteins and 267 metabolites in these lines. The HPBL showed the enrichment of proteins associated with protein synthesis, processing, and storage in addition to higher content of primary and secondary metabolites. The mapping of metabolites on the global metabolic pathways revealed the increased protein content in the HPBL that may have resulted from increased arginine content routed from citrulline-argininosuccinic acid. This information will be useful for breeders and biotechnologists to produce a value-added soybean trait. |
| format | Article |
| id | doaj-art-c5d7cfa0284b4ef88cdb1eb35a66cdeb |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-c5d7cfa0284b4ef88cdb1eb35a66cdeb2025-08-20T02:49:04ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-011810145510.1016/j.jafr.2024.101455Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analysesNazrul Islam0Qijian Song1Savithiry Natarajan2Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, USASoybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, USACorresponding author.; Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, USAWe have created several high protein (HPBL) and low protein breeding lines (LPBL) by crossing between NC-Raleigh and PI407228. The NC-Raleigh is a low protein cultivar widely grown in North Carolina with excellent yield potential and a high protein wild soybean (Glycine soja) with low yield. We analyzed the F6 generation of this cross for protein and oil compositions using near-infrared spectroscopy (NIR). Two soybean lines HPBL and LPBL were selected for biochemical analysis using mass spectrometry-based proteomic and metabolomic techniques. We identified 4538 proteins and 267 metabolites in these lines. The HPBL showed the enrichment of proteins associated with protein synthesis, processing, and storage in addition to higher content of primary and secondary metabolites. The mapping of metabolites on the global metabolic pathways revealed the increased protein content in the HPBL that may have resulted from increased arginine content routed from citrulline-argininosuccinic acid. This information will be useful for breeders and biotechnologists to produce a value-added soybean trait.http://www.sciencedirect.com/science/article/pii/S2666154324004927SoybeanProteomicsMetabolomicsMass spectrometrySeedPathways |
| spellingShingle | Nazrul Islam Qijian Song Savithiry Natarajan Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses Journal of Agriculture and Food Research Soybean Proteomics Metabolomics Mass spectrometry Seed Pathways |
| title | Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses |
| title_full | Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses |
| title_fullStr | Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses |
| title_full_unstemmed | Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses |
| title_short | Characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses |
| title_sort | characterization of high protein soybean using mass spectrometry based proteomic and metabolomic analyses |
| topic | Soybean Proteomics Metabolomics Mass spectrometry Seed Pathways |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324004927 |
| work_keys_str_mv | AT nazrulislam characterizationofhighproteinsoybeanusingmassspectrometrybasedproteomicandmetabolomicanalyses AT qijiansong characterizationofhighproteinsoybeanusingmassspectrometrybasedproteomicandmetabolomicanalyses AT savithirynatarajan characterizationofhighproteinsoybeanusingmassspectrometrybasedproteomicandmetabolomicanalyses |