Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions

Research on young trees’ adaptation to shade has predominantly focused on leaf-level responses, overlooking critical structural and functional adaptations in branch systems. In this study, we address this gap by investigating hierarchical branch morphology–physiology integration in 20-year-old <i...

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Main Authors: Bei Li, Wenkai Li, Sudipta Saha, Xiao Ma, Yang Liu, Haibo Wu, Peng Zhang, Hailong Shen
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/11/7/844
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author Bei Li
Wenkai Li
Sudipta Saha
Xiao Ma
Yang Liu
Haibo Wu
Peng Zhang
Hailong Shen
author_facet Bei Li
Wenkai Li
Sudipta Saha
Xiao Ma
Yang Liu
Haibo Wu
Peng Zhang
Hailong Shen
author_sort Bei Li
collection DOAJ
description Research on young trees’ adaptation to shade has predominantly focused on leaf-level responses, overlooking critical structural and functional adaptations in branch systems. In this study, we address this gap by investigating hierarchical branch morphology–physiology integration in 20-year-old <i>Pinus koraiensis</i> specimens across four distinct light conditions classified by photosynthetic photon flux density (PPFD): three in the understory (low light, LL: 0–25 μmol/m<sup>2</sup>/s; moderate light, ML: 25–50 μmol/m<sup>2</sup>/s; and high levels of light, HL: 50–100 μmol/m<sup>2</sup>/s) and one under full light as a control (FL: 1300–1700 μmol/m<sup>2</sup>/s). We measured branch base diameter, length, and angle as well as chlorophyll and NSCs content in branches and needles. Branch base diameter and length were more than 1.5-fold higher in the FL Korean pine trees compared to the understory-grown ones, while the branching angle and ratio in the LL Korean pine trees were more than two times greater than those in the FL trees. As light levels increased, Chlorophyll a and b and total chlorophyll (Chla, Chlb, and Chl) concentrations in the needles all significantly decreased. Starch, glucose, and NSC (Starch + Soluble Sugars) concentrations in both needles and branches were the highest in the trees under FL and lowest under ML (except for soluble sugars in branches). Understory young <i>P. koraiensis</i> trees morphologically and physiologically adapt to limited light conditions, growing to be more horizontal, synthesizing more chlorophyll in needles, and attempting to increase their light-foraging ability. We recommend gradually expanding growing spaces to increase light availability for 20-year-old Korean pine trees grown under canopy level.
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spelling doaj-art-c5cb1b357d954c128ef2d5e301da418d2025-08-20T03:58:26ZengMDPI AGHorticulturae2311-75242025-07-0111784410.3390/horticulturae11070844Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light ConditionsBei Li0Wenkai Li1Sudipta Saha2Xiao Ma3Yang Liu4Haibo Wu5Peng Zhang6Hailong Shen7College of Forestry, Northeast Forestry University, Harbin 150040, ChinaJingyu County Natural Resources and Forestry Bureau, Baishan 134300, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaSchool of Geography and Tourism, Harbin University, Harbin 150086, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaResearch on young trees’ adaptation to shade has predominantly focused on leaf-level responses, overlooking critical structural and functional adaptations in branch systems. In this study, we address this gap by investigating hierarchical branch morphology–physiology integration in 20-year-old <i>Pinus koraiensis</i> specimens across four distinct light conditions classified by photosynthetic photon flux density (PPFD): three in the understory (low light, LL: 0–25 μmol/m<sup>2</sup>/s; moderate light, ML: 25–50 μmol/m<sup>2</sup>/s; and high levels of light, HL: 50–100 μmol/m<sup>2</sup>/s) and one under full light as a control (FL: 1300–1700 μmol/m<sup>2</sup>/s). We measured branch base diameter, length, and angle as well as chlorophyll and NSCs content in branches and needles. Branch base diameter and length were more than 1.5-fold higher in the FL Korean pine trees compared to the understory-grown ones, while the branching angle and ratio in the LL Korean pine trees were more than two times greater than those in the FL trees. As light levels increased, Chlorophyll a and b and total chlorophyll (Chla, Chlb, and Chl) concentrations in the needles all significantly decreased. Starch, glucose, and NSC (Starch + Soluble Sugars) concentrations in both needles and branches were the highest in the trees under FL and lowest under ML (except for soluble sugars in branches). Understory young <i>P. koraiensis</i> trees morphologically and physiologically adapt to limited light conditions, growing to be more horizontal, synthesizing more chlorophyll in needles, and attempting to increase their light-foraging ability. We recommend gradually expanding growing spaces to increase light availability for 20-year-old Korean pine trees grown under canopy level.https://www.mdpi.com/2311-7524/11/7/844light intensity<i>Pinus koraiensis</i>non-structural carbohydratesphotosynthetic pigment
spellingShingle Bei Li
Wenkai Li
Sudipta Saha
Xiao Ma
Yang Liu
Haibo Wu
Peng Zhang
Hailong Shen
Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions
Horticulturae
light intensity
<i>Pinus koraiensis</i>
non-structural carbohydrates
photosynthetic pigment
title Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions
title_full Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions
title_fullStr Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions
title_full_unstemmed Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions
title_short Hierarchic Branch Morphology, Needle Chlorophyll Content, and Needle and Branch Non-Structural Carbohydrate Concentrations (NSCs) Imply Young <i>Pinus koraiensis</i> Trees Exhibit Diverse Responses Under Different Light Conditions
title_sort hierarchic branch morphology needle chlorophyll content and needle and branch non structural carbohydrate concentrations nscs imply young i pinus koraiensis i trees exhibit diverse responses under different light conditions
topic light intensity
<i>Pinus koraiensis</i>
non-structural carbohydrates
photosynthetic pigment
url https://www.mdpi.com/2311-7524/11/7/844
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