Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions
Abstract Nucleic acid (NA) testing at the point‐of‐care requires efficient NA extraction followed by post‐NA amplification to achieve necessary detection sensitivity. Nanofibers (NFs) are demonstrated to be an ideal solid surface in an NA extraction process but necessitate harsh conditions that inte...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-10-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400329 |
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| author | Alissa J. Wieberneit Nongnoot Wongkaew Antje J. Baeumner |
| author_facet | Alissa J. Wieberneit Nongnoot Wongkaew Antje J. Baeumner |
| author_sort | Alissa J. Wieberneit |
| collection | DOAJ |
| description | Abstract Nucleic acid (NA) testing at the point‐of‐care requires efficient NA extraction followed by post‐NA amplification to achieve necessary detection sensitivity. Nanofibers (NFs) are demonstrated to be an ideal solid surface in an NA extraction process but necessitate harsh conditions that interfere with the subsequent NA amplification process. It is demonstrated that novel, pH tunable, zwitterionic NFs composed of uncharged nylon doped with the weakly basic, cationic polyallylamine hydrochloride and the weakly acidic anionic polycarboxylic acid to address the issue. Unlike the other cationic polymers investigated, e.g. polybrene and polyaniline, these polymers allow efficient NA extraction in Tris‐ethylenediamine tetra‐acetic acid buffer under mild conditions (pH 4.5 containing 0.1% Tween 20 for adsorption, and pH 10 with 50 mM NaCl for elution). Adsorption and elution yields over 95% and 70%, respectively, are achieved. It also discovered a correlation between material morphologies and the NA extraction suggests that the combination of polymer chemistries and nanofiber morphologies facilitates efficient NA extraction at low concentrations (ng range) within a short time period (<10 min). Considering the simple protocols and instrument‐free operation the as‐developed NFs are highly attractive for use in sample‐to‐answer NA testing in point‐of‐care settings. |
| format | Article |
| id | doaj-art-c5c83c8e92b6431faf739dbf3abd35d8 |
| institution | OA Journals |
| issn | 2196-7350 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley-VCH |
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| series | Advanced Materials Interfaces |
| spelling | doaj-art-c5c83c8e92b6431faf739dbf3abd35d82025-08-20T01:54:16ZengWiley-VCHAdvanced Materials Interfaces2196-73502024-10-011130n/an/a10.1002/admi.202400329Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild ConditionsAlissa J. Wieberneit0Nongnoot Wongkaew1Antje J. Baeumner2Institute of Analytical Chemistry Chemo‐ and Biosensors University of Regensburg Universitaetsstraße 31 93053 Regensburg GermanyInstitute of Analytical Chemistry Chemo‐ and Biosensors University of Regensburg Universitaetsstraße 31 93053 Regensburg GermanyInstitute of Analytical Chemistry Chemo‐ and Biosensors University of Regensburg Universitaetsstraße 31 93053 Regensburg GermanyAbstract Nucleic acid (NA) testing at the point‐of‐care requires efficient NA extraction followed by post‐NA amplification to achieve necessary detection sensitivity. Nanofibers (NFs) are demonstrated to be an ideal solid surface in an NA extraction process but necessitate harsh conditions that interfere with the subsequent NA amplification process. It is demonstrated that novel, pH tunable, zwitterionic NFs composed of uncharged nylon doped with the weakly basic, cationic polyallylamine hydrochloride and the weakly acidic anionic polycarboxylic acid to address the issue. Unlike the other cationic polymers investigated, e.g. polybrene and polyaniline, these polymers allow efficient NA extraction in Tris‐ethylenediamine tetra‐acetic acid buffer under mild conditions (pH 4.5 containing 0.1% Tween 20 for adsorption, and pH 10 with 50 mM NaCl for elution). Adsorption and elution yields over 95% and 70%, respectively, are achieved. It also discovered a correlation between material morphologies and the NA extraction suggests that the combination of polymer chemistries and nanofiber morphologies facilitates efficient NA extraction at low concentrations (ng range) within a short time period (<10 min). Considering the simple protocols and instrument‐free operation the as‐developed NFs are highly attractive for use in sample‐to‐answer NA testing in point‐of‐care settings.https://doi.org/10.1002/admi.202400329anion‐exchangenanofibersnucleic acidspH‐tunable surface chargepoint‐of‐caresolid‐phase extraction |
| spellingShingle | Alissa J. Wieberneit Nongnoot Wongkaew Antje J. Baeumner Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions Advanced Materials Interfaces anion‐exchange nanofibers nucleic acids pH‐tunable surface charge point‐of‐care solid‐phase extraction |
| title | Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions |
| title_full | Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions |
| title_fullStr | Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions |
| title_full_unstemmed | Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions |
| title_short | Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions |
| title_sort | novel electrospun zwitterionic nanofibers for point of care nucleic acid isolation strategies under mild conditions |
| topic | anion‐exchange nanofibers nucleic acids pH‐tunable surface charge point‐of‐care solid‐phase extraction |
| url | https://doi.org/10.1002/admi.202400329 |
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