Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene
In order to study the tissue expression character of Follistatin gene in marine fish, the promoter of Paralichthys olivaceus Follistatin gene was isolated by PCR, and the sequence was analyzed; the function of Follistatin gene on the development of muscle was studied by RT-PCR and microinjection. Th...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2007-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0616 |
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| author | LIU Qing-hua TAN Xun-gang XU Yong-li ZHANG Pei-jun ZHANG Yu-qing |
| author_facet | LIU Qing-hua TAN Xun-gang XU Yong-li ZHANG Pei-jun ZHANG Yu-qing |
| author_sort | LIU Qing-hua |
| collection | DOAJ |
| description | In order to study the tissue expression character of Follistatin gene in marine fish, the promoter of Paralichthys olivaceus Follistatin gene was isolated by PCR, and the sequence was analyzed; the function of Follistatin gene on the development of muscle was studied by RT-PCR and microinjection. The results indicated that the promoter had many transcription factor binding sites such as USF, E47, MyoD and NF-Y, et al; in Paralichthys olivaceus adult tissues, Follistatin expressed in body kidney, intestine, heart, head kidney and spleen, while did not express in muscle and liver; microinjection revealed that Follistatin gene played a role in the early development of muscle. |
| format | Article |
| id | doaj-art-c5c792c1e54f47ad99982c8ca25c5ec6 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2007-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c5c792c1e54f47ad99982c8ca25c5ec62025-08-20T03:16:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-11-013361662010.3785/1008-9209.2007.06.061610089209Cloning and analysis of promoter of Paralichthys olivaceus Follistatin geneLIU Qing-huaTAN Xun-gangXU Yong-liZHANG Pei-junZHANG Yu-qingIn order to study the tissue expression character of Follistatin gene in marine fish, the promoter of Paralichthys olivaceus Follistatin gene was isolated by PCR, and the sequence was analyzed; the function of Follistatin gene on the development of muscle was studied by RT-PCR and microinjection. The results indicated that the promoter had many transcription factor binding sites such as USF, E47, MyoD and NF-Y, et al; in Paralichthys olivaceus adult tissues, Follistatin expressed in body kidney, intestine, heart, head kidney and spleen, while did not express in muscle and liver; microinjection revealed that Follistatin gene played a role in the early development of muscle.https://www.academax.com/doi/10.3785/1008-9209.2007.06.0616<italic>Paralichthys olivaceus</italic>Follistatinpromotercloningdevelopment of muscle |
| spellingShingle | LIU Qing-hua TAN Xun-gang XU Yong-li ZHANG Pei-jun ZHANG Yu-qing Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene 浙江大学学报. 农业与生命科学版 <italic>Paralichthys olivaceus</italic> Follistatin promoter cloning development of muscle |
| title | Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene |
| title_full | Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene |
| title_fullStr | Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene |
| title_full_unstemmed | Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene |
| title_short | Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene |
| title_sort | cloning and analysis of promoter of paralichthys olivaceus follistatin gene |
| topic | <italic>Paralichthys olivaceus</italic> Follistatin promoter cloning development of muscle |
| url | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0616 |
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