Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation
The global surge in population and economic growth has intensified energy consumption reliant on fossil resources, leading to environmental degradation. Biogas, while offering a renewable alternative, requires CO2 purification. This study addresses the critical knowledge gap in integrated waste-to-v...
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Elsevier
2025-06-01
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| Series: | Results in Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590123025014537 |
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| author | Bambang Trisakti Rivaldi Sidabutar Irvan Irvan Erman Munir Maya Sarah Farida Hanum Mhd. Rivaldi Syahputra Michael Michael Vanness Vanness Gloria Clarita Sinamo Renata Ambarita Mawardyah Sidauruk Selvia Geraldine Silaban Yasmin Nabilah Hiroyuki Daimon Mohd. Sobri Takriff Peer Mohamed Abdul |
| author_facet | Bambang Trisakti Rivaldi Sidabutar Irvan Irvan Erman Munir Maya Sarah Farida Hanum Mhd. Rivaldi Syahputra Michael Michael Vanness Vanness Gloria Clarita Sinamo Renata Ambarita Mawardyah Sidauruk Selvia Geraldine Silaban Yasmin Nabilah Hiroyuki Daimon Mohd. Sobri Takriff Peer Mohamed Abdul |
| author_sort | Bambang Trisakti |
| collection | DOAJ |
| description | The global surge in population and economic growth has intensified energy consumption reliant on fossil resources, leading to environmental degradation. Biogas, while offering a renewable alternative, requires CO2 purification. This study addresses the critical knowledge gap in integrated waste-to-value approaches by investigating the synergistic coupling of biogas purification effluent utilization with Chlorella vulgaris USU1 cultivation for lipid production. This novel integration facilitates simultaneous high-quality biofuel generation and significant CO2 utilization, thereby enabling absorbent regeneration in a closed-loop system. Utilizing packed absorption column-derived effluent (PACDE) containing KHCO3-PZCOO- complex solutions as the nutrient medium, this study systematically optimized cultivation parameters to maximize both biomass production and carbon utilization efficiency. Under optimized conditions (24:0 light intensity, 50-rpm agitation, 0.6 initial absorbance), the system achieved 58.3 % CO2 biofixation efficiency with a maximum biomass concentration of 8.64 g/L. Biochemical characterization revealed a composition of 23 % carbohydrates and 56 % proteins, with the fatty acid profile predominantly comprising palmitic (29.7 %), oleic (27.6 %), and linoleic (24.1 %) acids. Notably, the USU1 strain demonstrated superior lipid accumulation capability with 31.19 % yield, surpassing previously reported values for comparable strains. Economic analysis established favorable metrics with capital costs of IDR 4505/L, operating expenses of IDR 1155/L, and profit gained of IDR 8991/L, yielding a net profit of IDR 3331/L. Mass balance assessment demonstrated valorization of carbon inputs with approximately 2.97 kg CO2 annually while generating 1175 L of lipid-rich microalgae oil and 3745 L of reusable biofertilizer effluent. This research establishes a foundation for scalable and closed-loop carbon capture and utilization technologies with implications for industrial zero-waste implementation and circular bioeconomy paradigms. |
| format | Article |
| id | doaj-art-c5c4dfd3f9a540199dbd4132955f6c4f |
| institution | Kabale University |
| issn | 2590-1230 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Results in Engineering |
| spelling | doaj-art-c5c4dfd3f9a540199dbd4132955f6c4f2025-08-20T03:53:47ZengElsevierResults in Engineering2590-12302025-06-012610538310.1016/j.rineng.2025.105383Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generationBambang Trisakti0Rivaldi Sidabutar1Irvan Irvan2Erman Munir3Maya Sarah4Farida Hanum5Mhd. Rivaldi Syahputra6Michael Michael7Vanness Vanness8Gloria Clarita Sinamo9Renata Ambarita10Mawardyah Sidauruk11Selvia Geraldine Silaban12Yasmin Nabilah13Hiroyuki Daimon14Mohd. Sobri Takriff15Peer Mohamed Abdul16Department of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, Indonesia; Waste-to-Industrial Sustainable Energy Center, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, Indonesia; Waste-to-Industrial Sustainable Energy Center, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, Indonesia; Waste-to-Industrial Sustainable Energy Center, Universitas Sumatera Utara, Medan 20155, Indonesia; Corresponding author.Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, Indonesia; Waste-to-Industrial Sustainable Energy Center, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, Indonesia; Waste-to-Industrial Sustainable Energy Center, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Chemical Engineering for Energy and Environment, KTH Royal Institute of Technology, SE-100 44 Stockholm, SwedenDepartment of Applied Chemistry and Life Science, Toyohashi University of Technology, Toyohashi 441-8580, JapanMechanical and Nuclear Engineering Department, College of Engineering, University of Sharjah, Sharjah Emirate 61467, United Arab EmiratesDepartment of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Selangor Darul Ehsan, Bangi 43600, MalaysiaThe global surge in population and economic growth has intensified energy consumption reliant on fossil resources, leading to environmental degradation. Biogas, while offering a renewable alternative, requires CO2 purification. This study addresses the critical knowledge gap in integrated waste-to-value approaches by investigating the synergistic coupling of biogas purification effluent utilization with Chlorella vulgaris USU1 cultivation for lipid production. This novel integration facilitates simultaneous high-quality biofuel generation and significant CO2 utilization, thereby enabling absorbent regeneration in a closed-loop system. Utilizing packed absorption column-derived effluent (PACDE) containing KHCO3-PZCOO- complex solutions as the nutrient medium, this study systematically optimized cultivation parameters to maximize both biomass production and carbon utilization efficiency. Under optimized conditions (24:0 light intensity, 50-rpm agitation, 0.6 initial absorbance), the system achieved 58.3 % CO2 biofixation efficiency with a maximum biomass concentration of 8.64 g/L. Biochemical characterization revealed a composition of 23 % carbohydrates and 56 % proteins, with the fatty acid profile predominantly comprising palmitic (29.7 %), oleic (27.6 %), and linoleic (24.1 %) acids. Notably, the USU1 strain demonstrated superior lipid accumulation capability with 31.19 % yield, surpassing previously reported values for comparable strains. Economic analysis established favorable metrics with capital costs of IDR 4505/L, operating expenses of IDR 1155/L, and profit gained of IDR 8991/L, yielding a net profit of IDR 3331/L. Mass balance assessment demonstrated valorization of carbon inputs with approximately 2.97 kg CO2 annually while generating 1175 L of lipid-rich microalgae oil and 3745 L of reusable biofertilizer effluent. This research establishes a foundation for scalable and closed-loop carbon capture and utilization technologies with implications for industrial zero-waste implementation and circular bioeconomy paradigms.http://www.sciencedirect.com/science/article/pii/S2590123025014537Biogas valorizationMicroalgae cultivationCO2 biofixationLipid productionIntegrated biorefinery |
| spellingShingle | Bambang Trisakti Rivaldi Sidabutar Irvan Irvan Erman Munir Maya Sarah Farida Hanum Mhd. Rivaldi Syahputra Michael Michael Vanness Vanness Gloria Clarita Sinamo Renata Ambarita Mawardyah Sidauruk Selvia Geraldine Silaban Yasmin Nabilah Hiroyuki Daimon Mohd. Sobri Takriff Peer Mohamed Abdul Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation Results in Engineering Biogas valorization Microalgae cultivation CO2 biofixation Lipid production Integrated biorefinery |
| title | Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation |
| title_full | Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation |
| title_fullStr | Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation |
| title_full_unstemmed | Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation |
| title_short | Sustainable lipid production from Chlorella vulgaris USU1 strain using packed absorption column-derived effluent as carbon source for biomass generation |
| title_sort | sustainable lipid production from chlorella vulgaris usu1 strain using packed absorption column derived effluent as carbon source for biomass generation |
| topic | Biogas valorization Microalgae cultivation CO2 biofixation Lipid production Integrated biorefinery |
| url | http://www.sciencedirect.com/science/article/pii/S2590123025014537 |
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