Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection
This study was conducted to extract, isolate and quantify Quercetin from Iraqi green walnut husk (GWH) using a novel ionic liquid-based digestion extraction and gel filtration procedure coupled with visible spectrophotometry detection and compared with a novel microfluidic paper-based analytical de...
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University of Baghdad, College of Science for Women
2024-09-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9227 |
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| author | Murtadha A. Mohammad Jwan Oday Abdulsattar Zaizafoon N. Nasif Nasif |
| author_facet | Murtadha A. Mohammad Jwan Oday Abdulsattar Zaizafoon N. Nasif Nasif |
| author_sort | Murtadha A. Mohammad |
| collection | DOAJ |
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This study was conducted to extract, isolate and quantify Quercetin from Iraqi green walnut husk (GWH) using a novel ionic liquid-based digestion extraction and gel filtration procedure coupled with visible spectrophotometry detection and compared with a novel microfluidic paper-based analytical device (µPADs) aiming for an economic, and green method. 1-butyl-3-methylimidazolium chloride (BMIMCl) aqueous solution was used as a green solvent alternative to conventional organic solvents due to their impressive solvation properties. The results showed that 100 mg of plant husk required 15 mL of BMIMCl solvent, and 30 minutes of digestion at 75° C, which involves a simpler, and greener extraction method compared with other extraction methods. Under optimal conditions, Quercetin was isolated using a gel filtration technique, and further investigations were conducted using high-performance liquid chromatography and Fourier-transmitted infrared to confirm the isolation of Quercetin. Aluminum chloride assay was selected to determine the concentration of Quercetin giving a linearity of 10-100 mg L-1 and correlation coefficient of 0.9369 and 0.7917 for spectrophotometric and µPADs methods, respectively. The antioxidant activity of isolated Quercetin showed good antioxidant activity and the ANOVA test was used to compare µPADs method with the spectrophotometric method and the results showed that there was no statistically significant difference between the two methods. In conclusion, BMIMCl solvent provides an excellent safe and suitable solvent for the extraction of phytochemicals from botanical drugs. In addition, the µPADs platform has been approved for its superiority against traditional methods for analytical purposes due to its green characteristic.
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| format | Article |
| id | doaj-art-c5c33fbc3df04872bff2a43b5091176b |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-c5c33fbc3df04872bff2a43b5091176b2025-08-20T03:57:43ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862024-09-0121910.21123/bsj.2024.9227Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform DetectionMurtadha A. Mohammad 0Jwan Oday Abdulsattar1https://orcid.org/0000-0001-8256-179XZaizafoon N. Nasif Nasif 2https://orcid.org/0000-0002-1758-7851Department of chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq.Department of chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq.Department of chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq. This study was conducted to extract, isolate and quantify Quercetin from Iraqi green walnut husk (GWH) using a novel ionic liquid-based digestion extraction and gel filtration procedure coupled with visible spectrophotometry detection and compared with a novel microfluidic paper-based analytical device (µPADs) aiming for an economic, and green method. 1-butyl-3-methylimidazolium chloride (BMIMCl) aqueous solution was used as a green solvent alternative to conventional organic solvents due to their impressive solvation properties. The results showed that 100 mg of plant husk required 15 mL of BMIMCl solvent, and 30 minutes of digestion at 75° C, which involves a simpler, and greener extraction method compared with other extraction methods. Under optimal conditions, Quercetin was isolated using a gel filtration technique, and further investigations were conducted using high-performance liquid chromatography and Fourier-transmitted infrared to confirm the isolation of Quercetin. Aluminum chloride assay was selected to determine the concentration of Quercetin giving a linearity of 10-100 mg L-1 and correlation coefficient of 0.9369 and 0.7917 for spectrophotometric and µPADs methods, respectively. The antioxidant activity of isolated Quercetin showed good antioxidant activity and the ANOVA test was used to compare µPADs method with the spectrophotometric method and the results showed that there was no statistically significant difference between the two methods. In conclusion, BMIMCl solvent provides an excellent safe and suitable solvent for the extraction of phytochemicals from botanical drugs. In addition, the µPADs platform has been approved for its superiority against traditional methods for analytical purposes due to its green characteristic. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9227Antioxidant, Green extraction, Green walnut husk, Ionic liquids, Microfluidic paper-based analytical device, Quercetin |
| spellingShingle | Murtadha A. Mohammad Jwan Oday Abdulsattar Zaizafoon N. Nasif Nasif Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection مجلة بغداد للعلوم Antioxidant, Green extraction, Green walnut husk, Ionic liquids, Microfluidic paper-based analytical device, Quercetin |
| title | Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection |
| title_full | Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection |
| title_fullStr | Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection |
| title_full_unstemmed | Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection |
| title_short | Determination of Quercetin in Iraqi Green Walnut Husk via Green Ionic Liquid-Based Digestion Extraction and Gel Filtration Procedures-Spectrophotometry and Microfluidic Paper-Based Analytical Platform Detection |
| title_sort | determination of quercetin in iraqi green walnut husk via green ionic liquid based digestion extraction and gel filtration procedures spectrophotometry and microfluidic paper based analytical platform detection |
| topic | Antioxidant, Green extraction, Green walnut husk, Ionic liquids, Microfluidic paper-based analytical device, Quercetin |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9227 |
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