Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays
The enzyme-linked immunospot (ELISPOT) assay has advanced into a useful and widely applicable tool for the evaluation of T-cell responses in both humans and animal models of diseases and/or vaccine candidates. Using synthetic peptides (either individually or as overlapping peptide mixtures) or whole...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/637649 |
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| author | Sandra A. Calarota Fausto Baldanti |
| author_facet | Sandra A. Calarota Fausto Baldanti |
| author_sort | Sandra A. Calarota |
| collection | DOAJ |
| description | The enzyme-linked immunospot (ELISPOT) assay has advanced into a useful and widely applicable tool for the evaluation of T-cell responses in both humans and animal models of diseases and/or vaccine candidates. Using synthetic peptides (either individually or as overlapping peptide mixtures) or whole antigens, total lymphocyte or isolated T-cell subset responses can be assessed either after short-term stimulation (standard ELISPOT) or after their expansion during a 10-day culture (cultured ELISPOT). Both assays detect different antigen-specific immune responses allowing the analysis of effector memory T cells and central memory T cells. This paper describes the principle of ELISPOT assays and discusses their application in the evaluation of immune correlates of clinical interest with a focus on the vaccine field. |
| format | Article |
| id | doaj-art-c5c0ee49ae014de0882e14792247cc96 |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-c5c0ee49ae014de0882e14792247cc962025-08-20T02:18:38ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/637649637649Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot AssaysSandra A. Calarota0Fausto Baldanti1S. S. Virologia Molecolare, S. C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Via Taramelli 5, 27100 Pavia, ItalyS. S. Virologia Molecolare, S. C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Via Taramelli 5, 27100 Pavia, ItalyThe enzyme-linked immunospot (ELISPOT) assay has advanced into a useful and widely applicable tool for the evaluation of T-cell responses in both humans and animal models of diseases and/or vaccine candidates. Using synthetic peptides (either individually or as overlapping peptide mixtures) or whole antigens, total lymphocyte or isolated T-cell subset responses can be assessed either after short-term stimulation (standard ELISPOT) or after their expansion during a 10-day culture (cultured ELISPOT). Both assays detect different antigen-specific immune responses allowing the analysis of effector memory T cells and central memory T cells. This paper describes the principle of ELISPOT assays and discusses their application in the evaluation of immune correlates of clinical interest with a focus on the vaccine field.http://dx.doi.org/10.1155/2013/637649 |
| spellingShingle | Sandra A. Calarota Fausto Baldanti Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays Clinical and Developmental Immunology |
| title | Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays |
| title_full | Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays |
| title_fullStr | Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays |
| title_full_unstemmed | Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays |
| title_short | Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays |
| title_sort | enumeration and characterization of human memory t cells by enzyme linked immunospot assays |
| url | http://dx.doi.org/10.1155/2013/637649 |
| work_keys_str_mv | AT sandraacalarota enumerationandcharacterizationofhumanmemorytcellsbyenzymelinkedimmunospotassays AT faustobaldanti enumerationandcharacterizationofhumanmemorytcellsbyenzymelinkedimmunospotassays |