Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy

Abstract Molecular phototheranostic dyes are of eminent interest for oncological diagnosis and imaging‐guided phototherapy. However, it remains challenging to develop photosensitizers (PSs) that simultaneously integrate high‐contrast photoacoustic imaging and efficient therapeutic capabilities. In t...

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Main Authors: Haiqiao Huang, Yingnan Wu, Xin He, Yahang Liu, Jing‐Hui Zhu, Mingrui Gu, Danhong Zhou, Saran Long, Yahui Chen, Lei Wang, Mingle Li, Xiaoqiang Chen, Xiaojun Peng
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202416905
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author Haiqiao Huang
Yingnan Wu
Xin He
Yahang Liu
Jing‐Hui Zhu
Mingrui Gu
Danhong Zhou
Saran Long
Yahui Chen
Lei Wang
Mingle Li
Xiaoqiang Chen
Xiaojun Peng
author_facet Haiqiao Huang
Yingnan Wu
Xin He
Yahang Liu
Jing‐Hui Zhu
Mingrui Gu
Danhong Zhou
Saran Long
Yahui Chen
Lei Wang
Mingle Li
Xiaoqiang Chen
Xiaojun Peng
author_sort Haiqiao Huang
collection DOAJ
description Abstract Molecular phototheranostic dyes are of eminent interest for oncological diagnosis and imaging‐guided phototherapy. However, it remains challenging to develop photosensitizers (PSs) that simultaneously integrate high‐contrast photoacoustic imaging and efficient therapeutic capabilities. In this work, a supramolecular strategy is employed to construct a molecular pair phototheranostic agent via the direct self‐assembly of two cyanines, C5TNa (anionic) and Cy‐Et (cationic). The Coulombic interactions between C5TNa and Cy‐Et facilitate the formation of a complementary cyanine pair (C5T‐ET) and the creation of supramolecular CT‐J‐type aggregates in water. This complementary cyanine pair (C5T‐ET) results in completely quenched fluorescence and significantly enhances nonradiative deactivation (≈22 ps), leading to a 3.3‐fold increase in photothermal conversion efficiency and a 7.1‐fold enhancement in photoacoustic response compared to indocyanine green (ICG). As a result, the J‐type aggregate cyanine pair (C5T‐ET) demonstrates high photoacoustic imaging capability and remarkable antitumor phototheranostic efficacy in vivo, highlighting its potential for clinical applications. This work provides strong experimental evidence for the superior performance of the complementary cyanine pair (C5T‐ET) in enhancing photosensitization and photoacoustic response. It is believed that this strategy will propel the advancement of controllable dye J‐aggregates and contribute to the practical implementation of photoacoustic imaging and phototherapy in vivo.
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spelling doaj-art-c5b445c6d7ec4629a4c4f7f7c2fa13db2025-08-20T01:51:39ZengWileyAdvanced Science2198-38442025-04-011213n/an/a10.1002/advs.202416905Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal TherapyHaiqiao Huang0Yingnan Wu1Xin He2Yahang Liu3Jing‐Hui Zhu4Mingrui Gu5Danhong Zhou6Saran Long7Yahui Chen8Lei Wang9Mingle Li10Xiaoqiang Chen11Xiaojun Peng12College of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaState Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116024 P. R. ChinaState Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116024 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaCollege of Materials Science and Engineering Shenzhen University Shenzhen 518060 P. R. ChinaAbstract Molecular phototheranostic dyes are of eminent interest for oncological diagnosis and imaging‐guided phototherapy. However, it remains challenging to develop photosensitizers (PSs) that simultaneously integrate high‐contrast photoacoustic imaging and efficient therapeutic capabilities. In this work, a supramolecular strategy is employed to construct a molecular pair phototheranostic agent via the direct self‐assembly of two cyanines, C5TNa (anionic) and Cy‐Et (cationic). The Coulombic interactions between C5TNa and Cy‐Et facilitate the formation of a complementary cyanine pair (C5T‐ET) and the creation of supramolecular CT‐J‐type aggregates in water. This complementary cyanine pair (C5T‐ET) results in completely quenched fluorescence and significantly enhances nonradiative deactivation (≈22 ps), leading to a 3.3‐fold increase in photothermal conversion efficiency and a 7.1‐fold enhancement in photoacoustic response compared to indocyanine green (ICG). As a result, the J‐type aggregate cyanine pair (C5T‐ET) demonstrates high photoacoustic imaging capability and remarkable antitumor phototheranostic efficacy in vivo, highlighting its potential for clinical applications. This work provides strong experimental evidence for the superior performance of the complementary cyanine pair (C5T‐ET) in enhancing photosensitization and photoacoustic response. It is believed that this strategy will propel the advancement of controllable dye J‐aggregates and contribute to the practical implementation of photoacoustic imaging and phototherapy in vivo.https://doi.org/10.1002/advs.202416905co‐assemblycyanine pairj aggregatesphotothermal therapyphotoacoustic imaging
spellingShingle Haiqiao Huang
Yingnan Wu
Xin He
Yahang Liu
Jing‐Hui Zhu
Mingrui Gu
Danhong Zhou
Saran Long
Yahui Chen
Lei Wang
Mingle Li
Xiaoqiang Chen
Xiaojun Peng
Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy
Advanced Science
co‐assembly
cyanine pair
j aggregates
photothermal therapy
photoacoustic imaging
title Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy
title_full Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy
title_fullStr Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy
title_full_unstemmed Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy
title_short Electrostatic Co‐Assembly of Cyanine Pair for Augmented Photoacoustic Imaging and Photothermal Therapy
title_sort electrostatic co assembly of cyanine pair for augmented photoacoustic imaging and photothermal therapy
topic co‐assembly
cyanine pair
j aggregates
photothermal therapy
photoacoustic imaging
url https://doi.org/10.1002/advs.202416905
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