Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China

Arbuscular mycorrhizal (AM) fungi are root symbionts that play an important role in the growth of vascular plants. Four AM fungi, including three new species, <i>Acaulospora citrusnsis</i>, <i>A. guangxiensis</i>, <i>A. jiangxiensis</i>, and a new country record f...

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Main Authors: Haisi Huang, Xiaojuan Qin, Yihao Kang, Jie Xu, Pengxiang Shang, Tingsu Chen, Tong Cheng, Jinlian Zhang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/11/5/382
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author Haisi Huang
Xiaojuan Qin
Yihao Kang
Jie Xu
Pengxiang Shang
Tingsu Chen
Tong Cheng
Jinlian Zhang
author_facet Haisi Huang
Xiaojuan Qin
Yihao Kang
Jie Xu
Pengxiang Shang
Tingsu Chen
Tong Cheng
Jinlian Zhang
author_sort Haisi Huang
collection DOAJ
description Arbuscular mycorrhizal (AM) fungi are root symbionts that play an important role in the growth of vascular plants. Four AM fungi, including three new species, <i>Acaulospora citrusnsis</i>, <i>A. guangxiensis</i>, <i>A. jiangxiensis</i>, and a new country record from China, <i>Acaulospora herrerae</i>, are reported based on morphological characteristics and molecular phylogenetic analysis. They were isolated and propagated from spores extracted from the rhizosphere soils of citrus. <i>A</i>. <i>citrusnsis</i> is characterized by forming hyaline to pale yellow globose to subglobose spores of (70.0–)85.0(−100.0) μm in diameter. Spores of <i>A. guangxiensis</i> are pale yellow to pale yellowish brown, with spherical to sub-spherical appearance and (103.1–)122.1(–147.1) μm in diameter. Young spores of <i>A. jiangxiensis</i> are hyaline, gradually turning pale yellow as they mature, with spherical to sub-spherical appearance and (78.7–)85.6(–90.0) μm in diameter. Spores of <i>A. herrerae</i> are hyaline and 86.3–127.2 μm in diameter. Four species have three spore wall layers, and spores form individually in the soil. The phylogenetic tree was constructed and inferred from sequences of 18S-ITS1-5.8S-ITS2-28S datasets by Bayesian inference and maximum likelihood analysis. Voucher specimens are deposited in the Center for Subtropical Arbuscular Mycorrhizal Fungi Culture Collection (CSMC).
format Article
id doaj-art-c5aa960946e5437cbabd0453fd790b80
institution Kabale University
issn 2309-608X
language English
publishDate 2025-05-01
publisher MDPI AG
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series Journal of Fungi
spelling doaj-art-c5aa960946e5437cbabd0453fd790b802025-08-20T03:47:54ZengMDPI AGJournal of Fungi2309-608X2025-05-0111538210.3390/jof11050382Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South ChinaHaisi Huang0Xiaojuan Qin1Yihao Kang2Jie Xu3Pengxiang Shang4Tingsu Chen5Tong Cheng6Jinlian Zhang7Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaMicrobiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSchool of Public Health, Xiamen University, Xiamen 361102, ChinaMicrobiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSchool of Public Health, Xiamen University, Xiamen 361102, ChinaMicrobiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSchool of Life Sciences, Xiamen University, Xiamen 361102, ChinaMicrobiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaArbuscular mycorrhizal (AM) fungi are root symbionts that play an important role in the growth of vascular plants. Four AM fungi, including three new species, <i>Acaulospora citrusnsis</i>, <i>A. guangxiensis</i>, <i>A. jiangxiensis</i>, and a new country record from China, <i>Acaulospora herrerae</i>, are reported based on morphological characteristics and molecular phylogenetic analysis. They were isolated and propagated from spores extracted from the rhizosphere soils of citrus. <i>A</i>. <i>citrusnsis</i> is characterized by forming hyaline to pale yellow globose to subglobose spores of (70.0–)85.0(−100.0) μm in diameter. Spores of <i>A. guangxiensis</i> are pale yellow to pale yellowish brown, with spherical to sub-spherical appearance and (103.1–)122.1(–147.1) μm in diameter. Young spores of <i>A. jiangxiensis</i> are hyaline, gradually turning pale yellow as they mature, with spherical to sub-spherical appearance and (78.7–)85.6(–90.0) μm in diameter. Spores of <i>A. herrerae</i> are hyaline and 86.3–127.2 μm in diameter. Four species have three spore wall layers, and spores form individually in the soil. The phylogenetic tree was constructed and inferred from sequences of 18S-ITS1-5.8S-ITS2-28S datasets by Bayesian inference and maximum likelihood analysis. Voucher specimens are deposited in the Center for Subtropical Arbuscular Mycorrhizal Fungi Culture Collection (CSMC).https://www.mdpi.com/2309-608X/11/5/382glomeromycotaarbuscular mycorrhizal fungi<i>Acaulospora</i>phylogeneticstaxonomy
spellingShingle Haisi Huang
Xiaojuan Qin
Yihao Kang
Jie Xu
Pengxiang Shang
Tingsu Chen
Tong Cheng
Jinlian Zhang
Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China
Journal of Fungi
glomeromycota
arbuscular mycorrhizal fungi
<i>Acaulospora</i>
phylogenetics
taxonomy
title Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China
title_full Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China
title_fullStr Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China
title_full_unstemmed Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China
title_short Three New Species and a New Record of Arbuscular Mycorrhizal Fungi of the Genus <i>Acaulospora</i> Associated with Citrus from South China
title_sort three new species and a new record of arbuscular mycorrhizal fungi of the genus i acaulospora i associated with citrus from south china
topic glomeromycota
arbuscular mycorrhizal fungi
<i>Acaulospora</i>
phylogenetics
taxonomy
url https://www.mdpi.com/2309-608X/11/5/382
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