Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania
Abstract Background The presence of a community effect in cluster randomized trials of malaria vector control interventions has led to the implementation of “buffer zones” around clusters to limit the potential for contamination between interventions. No consensus has been reached on how large these...
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2025-06-01
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| Online Access: | https://doi.org/10.1186/s12936-025-05438-y |
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| author | Charles Thickstun Eliud Lukole Jacklin F. Mosha Alphaxard Manjurano Immo Kleinschmidt Franklin W. Mosha Jacques Derek Charlwood Mark Rowland Ann Jolly Alice Zwerling Natacha Protopopoff Manisha Kulkarni |
| author_facet | Charles Thickstun Eliud Lukole Jacklin F. Mosha Alphaxard Manjurano Immo Kleinschmidt Franklin W. Mosha Jacques Derek Charlwood Mark Rowland Ann Jolly Alice Zwerling Natacha Protopopoff Manisha Kulkarni |
| author_sort | Charles Thickstun |
| collection | DOAJ |
| description | Abstract Background The presence of a community effect in cluster randomized trials of malaria vector control interventions has led to the implementation of “buffer zones” around clusters to limit the potential for contamination between interventions. No consensus has been reached on how large these buffers need to be to encapsulate the effect. Methods Nested within a phase-III cluster randomized malaria vector control trial in Northwest Tanzania, this study aims to determine the presence and spatial range of community effects from long-lasting insecticidal net (LLIN) and indoor residual spraying (IRS) interventions on household-level malaria infection in trial clusters four months post-intervention. Effective spatial range estimates of intervention community effects were compared to the 300m buffer distance implemented to limit intervention spillover between clusters in the trial. Geographically-weighted adjusted odds of malaria infection in children aged 0.5–14 years were determined four months post community-level intervention with a randomized allocation comprising one of two LLIN products (OlysetTM LN: 1000mg/m2 permethrin or OlysetTM Plus LN: 400 + permethrin 800mg/m2) with either IRS (Actellic®300CS: 1000mg/m2 micro-encapsulated pirimiphos-methyl) or no IRS. Robust semivariances were calculated for each of 48 intervention clusters and fit to semivariogram models by Weighted Least Squares. Results 6440 children from 2785 households were included in the geographically-weighted logistic regression. Prevalence of Plasmodium falciparum infection was 45.9% in the study population. Twenty (20) clusters had significant residual effect ranges, 13 of which were fit to Sine Hole Effect models, indicating periodicity in the study area. Effective range estimates for the study area had a median value of 1210 m (IQR: 958–1691). Clusters with IRS had a higher median range value: 1535 m (IQR: 976–3398) than those without IRS: 1168m (IQR: 829–1504). Conclusions Significant semivariogram model range estimates extended beyond the trial buffer sizes by a median average of 868 m in LLIN intervention clusters and 1235 m for IRS clusters. This presents a contamination, or spillover, potential for all trialed intervention types that may reduce the statistical power to detect difference between trial arms. Future studies should consider the ranges of intervention effects and contamination potential between trial arms when designing buffer areas. |
| format | Article |
| id | doaj-art-c5a92ddde9364d9d8a9b080a7da76873 |
| institution | DOAJ |
| issn | 1475-2875 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
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| series | Malaria Journal |
| spelling | doaj-art-c5a92ddde9364d9d8a9b080a7da768732025-08-20T02:39:45ZengBMCMalaria Journal1475-28752025-06-0124111410.1186/s12936-025-05438-yInter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, TanzaniaCharles Thickstun0Eliud Lukole1Jacklin F. Mosha2Alphaxard Manjurano3Immo Kleinschmidt4Franklin W. Mosha5Jacques Derek Charlwood6Mark Rowland7Ann Jolly8Alice Zwerling9Natacha Protopopoff10Manisha Kulkarni11School of Epidemiology and Public Health, University of OttawaDepartment of Parasitology, National Institute for Medical Research, Mwanza Medical Research CentreDepartment of Parasitology, National Institute for Medical Research, Mwanza Medical Research CentreDepartment of Parasitology, National Institute for Medical Research, Mwanza Medical Research CentreMRC International Statistics and Epidemiology Group, London School of Hygiene and Tropical MedicineDepartment of Parasitology, Kilimanjaro Christian Medical University CollegeDepartment of Disease Control, London School of Hygiene and Tropical MedicineDepartment of Disease Control, London School of Hygiene and Tropical MedicineSchool of Epidemiology and Public Health, University of OttawaSchool of Epidemiology and Public Health, University of OttawaDepartment of Disease Control, London School of Hygiene and Tropical MedicineSchool of Epidemiology and Public Health, University of OttawaAbstract Background The presence of a community effect in cluster randomized trials of malaria vector control interventions has led to the implementation of “buffer zones” around clusters to limit the potential for contamination between interventions. No consensus has been reached on how large these buffers need to be to encapsulate the effect. Methods Nested within a phase-III cluster randomized malaria vector control trial in Northwest Tanzania, this study aims to determine the presence and spatial range of community effects from long-lasting insecticidal net (LLIN) and indoor residual spraying (IRS) interventions on household-level malaria infection in trial clusters four months post-intervention. Effective spatial range estimates of intervention community effects were compared to the 300m buffer distance implemented to limit intervention spillover between clusters in the trial. Geographically-weighted adjusted odds of malaria infection in children aged 0.5–14 years were determined four months post community-level intervention with a randomized allocation comprising one of two LLIN products (OlysetTM LN: 1000mg/m2 permethrin or OlysetTM Plus LN: 400 + permethrin 800mg/m2) with either IRS (Actellic®300CS: 1000mg/m2 micro-encapsulated pirimiphos-methyl) or no IRS. Robust semivariances were calculated for each of 48 intervention clusters and fit to semivariogram models by Weighted Least Squares. Results 6440 children from 2785 households were included in the geographically-weighted logistic regression. Prevalence of Plasmodium falciparum infection was 45.9% in the study population. Twenty (20) clusters had significant residual effect ranges, 13 of which were fit to Sine Hole Effect models, indicating periodicity in the study area. Effective range estimates for the study area had a median value of 1210 m (IQR: 958–1691). Clusters with IRS had a higher median range value: 1535 m (IQR: 976–3398) than those without IRS: 1168m (IQR: 829–1504). Conclusions Significant semivariogram model range estimates extended beyond the trial buffer sizes by a median average of 868 m in LLIN intervention clusters and 1235 m for IRS clusters. This presents a contamination, or spillover, potential for all trialed intervention types that may reduce the statistical power to detect difference between trial arms. Future studies should consider the ranges of intervention effects and contamination potential between trial arms when designing buffer areas.https://doi.org/10.1186/s12936-025-05438-yMalariaCommunity effectBufferSemivarianceSpatial analysis |
| spellingShingle | Charles Thickstun Eliud Lukole Jacklin F. Mosha Alphaxard Manjurano Immo Kleinschmidt Franklin W. Mosha Jacques Derek Charlwood Mark Rowland Ann Jolly Alice Zwerling Natacha Protopopoff Manisha Kulkarni Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania Malaria Journal Malaria Community effect Buffer Semivariance Spatial analysis |
| title | Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania |
| title_full | Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania |
| title_fullStr | Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania |
| title_full_unstemmed | Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania |
| title_short | Inter-cluster contamination: a semivariance analysis of community effect ranges of malaria vector control interventions in a four-armed malaria trial in Muleba, Tanzania |
| title_sort | inter cluster contamination a semivariance analysis of community effect ranges of malaria vector control interventions in a four armed malaria trial in muleba tanzania |
| topic | Malaria Community effect Buffer Semivariance Spatial analysis |
| url | https://doi.org/10.1186/s12936-025-05438-y |
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