Nitrite-driven anaerobic ethane oxidation
Ethane, the second most abundant gaseous hydrocarbon in vast anoxic environments, is an overlooked greenhouse gas. Microbial anaerobic oxidation of ethane can be driven by available electron acceptors such as sulfate and nitrate. However, despite nitrite being a more thermodynamically feasible elect...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-09-01
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| Series: | Environmental Science and Ecotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666498424000528 |
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| author | Cheng-Cheng Dang Yin-Zhu Jin Xin Tan Wen-Bo Nie Yang Lu Bing-Feng Liu De-Feng Xing Nan-Qi Ren Guo-Jun Xie |
| author_facet | Cheng-Cheng Dang Yin-Zhu Jin Xin Tan Wen-Bo Nie Yang Lu Bing-Feng Liu De-Feng Xing Nan-Qi Ren Guo-Jun Xie |
| author_sort | Cheng-Cheng Dang |
| collection | DOAJ |
| description | Ethane, the second most abundant gaseous hydrocarbon in vast anoxic environments, is an overlooked greenhouse gas. Microbial anaerobic oxidation of ethane can be driven by available electron acceptors such as sulfate and nitrate. However, despite nitrite being a more thermodynamically feasible electron acceptor than sulfate or nitrate, little is known about nitrite-driven anaerobic ethane oxidation. In this study, a microbial culture capable of nitrite-driven anaerobic ethane oxidation was enriched through the long-term operation of a nitrite-and-ethane-fed bioreactor. During continuous operation, the nitrite removal rate and the theoretical ethane oxidation rate remained stable at approximately 25.0 mg NO2–N L−1 d−1 and 11.48 mg C2H6 L−1 d−1, respectively. Batch tests demonstrated that ethane is essential for nitrite removal in this microbial culture. Metabolic function analysis revealed that a species affiliated with a novel genus within the family Rhodocyclaceae, designated as 'Candidatus Alkanivoras nitrosoreducens', may perform the nitrite-driven anaerobic ethane oxidation. In the proposed metabolic model, despite the absence of known genes for ethane conversion to ethyl-succinate and succinate-CoA ligase, 'Ca. A. nitrosoreducens' encodes a prospective fumarate addition pathway for anaerobic ethane oxidation and a complete denitrification pathway for nitrite reduction to nitrogen. These findings advance our understanding of nitrite-driven anaerobic ethane oxidation, highlighting the previously overlooked impact of anaerobic ethane oxidation in natural ecosystems. |
| format | Article |
| id | doaj-art-c5a816c0830c46be93af115e708e0ffd |
| institution | OA Journals |
| issn | 2666-4984 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Science and Ecotechnology |
| spelling | doaj-art-c5a816c0830c46be93af115e708e0ffd2025-08-20T01:54:46ZengElsevierEnvironmental Science and Ecotechnology2666-49842024-09-012110043810.1016/j.ese.2024.100438Nitrite-driven anaerobic ethane oxidationCheng-Cheng Dang0Yin-Zhu Jin1Xin Tan2Wen-Bo Nie3Yang Lu4Bing-Feng Liu5De-Feng Xing6Nan-Qi Ren7Guo-Jun Xie8State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, ChinaKey Laboratory of the Three Gorges Region's Eco-Environment, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing, 400044, ChinaWater Innovation and Smart Environment Laboratory, School of Civil and Environmental Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, Queensland, 4001, AustraliaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China; Corresponding author.Ethane, the second most abundant gaseous hydrocarbon in vast anoxic environments, is an overlooked greenhouse gas. Microbial anaerobic oxidation of ethane can be driven by available electron acceptors such as sulfate and nitrate. However, despite nitrite being a more thermodynamically feasible electron acceptor than sulfate or nitrate, little is known about nitrite-driven anaerobic ethane oxidation. In this study, a microbial culture capable of nitrite-driven anaerobic ethane oxidation was enriched through the long-term operation of a nitrite-and-ethane-fed bioreactor. During continuous operation, the nitrite removal rate and the theoretical ethane oxidation rate remained stable at approximately 25.0 mg NO2–N L−1 d−1 and 11.48 mg C2H6 L−1 d−1, respectively. Batch tests demonstrated that ethane is essential for nitrite removal in this microbial culture. Metabolic function analysis revealed that a species affiliated with a novel genus within the family Rhodocyclaceae, designated as 'Candidatus Alkanivoras nitrosoreducens', may perform the nitrite-driven anaerobic ethane oxidation. In the proposed metabolic model, despite the absence of known genes for ethane conversion to ethyl-succinate and succinate-CoA ligase, 'Ca. A. nitrosoreducens' encodes a prospective fumarate addition pathway for anaerobic ethane oxidation and a complete denitrification pathway for nitrite reduction to nitrogen. These findings advance our understanding of nitrite-driven anaerobic ethane oxidation, highlighting the previously overlooked impact of anaerobic ethane oxidation in natural ecosystems.http://www.sciencedirect.com/science/article/pii/S2666498424000528Anaerobic ethane oxidationDenitrificationMicrobial cultureFumarate addition pathwayGreenhouse gas |
| spellingShingle | Cheng-Cheng Dang Yin-Zhu Jin Xin Tan Wen-Bo Nie Yang Lu Bing-Feng Liu De-Feng Xing Nan-Qi Ren Guo-Jun Xie Nitrite-driven anaerobic ethane oxidation Environmental Science and Ecotechnology Anaerobic ethane oxidation Denitrification Microbial culture Fumarate addition pathway Greenhouse gas |
| title | Nitrite-driven anaerobic ethane oxidation |
| title_full | Nitrite-driven anaerobic ethane oxidation |
| title_fullStr | Nitrite-driven anaerobic ethane oxidation |
| title_full_unstemmed | Nitrite-driven anaerobic ethane oxidation |
| title_short | Nitrite-driven anaerobic ethane oxidation |
| title_sort | nitrite driven anaerobic ethane oxidation |
| topic | Anaerobic ethane oxidation Denitrification Microbial culture Fumarate addition pathway Greenhouse gas |
| url | http://www.sciencedirect.com/science/article/pii/S2666498424000528 |
| work_keys_str_mv | AT chengchengdang nitritedrivenanaerobicethaneoxidation AT yinzhujin nitritedrivenanaerobicethaneoxidation AT xintan nitritedrivenanaerobicethaneoxidation AT wenbonie nitritedrivenanaerobicethaneoxidation AT yanglu nitritedrivenanaerobicethaneoxidation AT bingfengliu nitritedrivenanaerobicethaneoxidation AT defengxing nitritedrivenanaerobicethaneoxidation AT nanqiren nitritedrivenanaerobicethaneoxidation AT guojunxie nitritedrivenanaerobicethaneoxidation |