Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients
Abstract Recurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) after liver transplantation (LT) is a continuing concern. The role of gut microbiome dysbiosis in MASLD initiation and progression has been well established. However, there is a lack of comprehensive gut microbio...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-04153-8 |
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| author | Goutam Banerjee Suraya Rahman Papri Hai Huang Sanjaya Kumar Satapathy Pratik Banerjee |
| author_facet | Goutam Banerjee Suraya Rahman Papri Hai Huang Sanjaya Kumar Satapathy Pratik Banerjee |
| author_sort | Goutam Banerjee |
| collection | DOAJ |
| description | Abstract Recurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) after liver transplantation (LT) is a continuing concern. The role of gut microbiome dysbiosis in MASLD initiation and progression has been well established. However, there is a lack of comprehensive gut microbiome shotgun sequence data for patients experiencing MASLD recurrence after LT. In this data descriptor, we describe a dataset of deep metagenomic sequences of a well-defined LT recipient population. Community-based analysis revealed a high abundance of Akkermansia muciniphila, consistently observed in most patient samples with a low (0–2) MASLD Activity Score (NAS). We constructed 357 metagenome-assembled genomes (MAGs), including 220 high-quality MAGs (>90% completion). The abundance of different species of Bacteroides MAGs dominated in patient samples with NAS > 5 (“definite MASH”). In contrast, the MAGs of A. muciniphila, Akkermansia sp., and Blutia sp. dominated in samples from patients without MASH (NAS = 0–2). In addition, the phylogenetic analysis of A. muciniphila and Akkermansia sp. MAGs identified two new phylogroups of Akkermansia that are distinct from the previously reported three phylogroups. |
| format | Article |
| id | doaj-art-c59dd4a3389341dcaf43386bd5d0e2b8 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Data |
| spelling | doaj-art-c59dd4a3389341dcaf43386bd5d0e2b82025-01-12T12:07:54ZengNature PortfolioScientific Data2052-44632025-01-0112111110.1038/s41597-024-04153-8Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patientsGoutam Banerjee0Suraya Rahman Papri1Hai Huang2Sanjaya Kumar Satapathy3Pratik Banerjee4Department of Food Science and Human Nutrition, University of Illinois at Urbana-ChampaignDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-ChampaignThe Feinstein Institutes for Medical Research, Northwell HealthDivision of Hepatology, Sandra Atlas Bass Center for Liver Diseases & Transplantation, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell HealthDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-ChampaignAbstract Recurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) after liver transplantation (LT) is a continuing concern. The role of gut microbiome dysbiosis in MASLD initiation and progression has been well established. However, there is a lack of comprehensive gut microbiome shotgun sequence data for patients experiencing MASLD recurrence after LT. In this data descriptor, we describe a dataset of deep metagenomic sequences of a well-defined LT recipient population. Community-based analysis revealed a high abundance of Akkermansia muciniphila, consistently observed in most patient samples with a low (0–2) MASLD Activity Score (NAS). We constructed 357 metagenome-assembled genomes (MAGs), including 220 high-quality MAGs (>90% completion). The abundance of different species of Bacteroides MAGs dominated in patient samples with NAS > 5 (“definite MASH”). In contrast, the MAGs of A. muciniphila, Akkermansia sp., and Blutia sp. dominated in samples from patients without MASH (NAS = 0–2). In addition, the phylogenetic analysis of A. muciniphila and Akkermansia sp. MAGs identified two new phylogroups of Akkermansia that are distinct from the previously reported three phylogroups.https://doi.org/10.1038/s41597-024-04153-8 |
| spellingShingle | Goutam Banerjee Suraya Rahman Papri Hai Huang Sanjaya Kumar Satapathy Pratik Banerjee Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients Scientific Data |
| title | Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients |
| title_full | Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients |
| title_fullStr | Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients |
| title_full_unstemmed | Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients |
| title_short | Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients |
| title_sort | deep sequencing derived metagenome assembled genomes from the gut microbiome of liver transplant patients |
| url | https://doi.org/10.1038/s41597-024-04153-8 |
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