Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas
Twenty two multifloral honey samples representing central western parts of Nepal were examined spectrophotometrically for their antioxidant properties and total phenol content. The modified Folin-Ciocalteu method was used to determine total phenol content and 2,2-diphenyl-1-picrylhydrazyl radicals (...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2015-06-01
|
| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/pjfns.2015.65.issue-2/pjfns-2015-0024/pjfns-2015-0024.xml?format=INT |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832570526017519616 |
|---|---|
| author | Neupane Bishnu Prasad Malla Komal Prasad Kaundinnyayana Atis Poudel Prakash Thapa Rashmi Shrestha Sabina |
| author_facet | Neupane Bishnu Prasad Malla Komal Prasad Kaundinnyayana Atis Poudel Prakash Thapa Rashmi Shrestha Sabina |
| author_sort | Neupane Bishnu Prasad |
| collection | DOAJ |
| description | Twenty two multifloral honey samples representing central western parts of Nepal were examined spectrophotometrically for their antioxidant properties and total phenol content. The modified Folin-Ciocalteu method was used to determine total phenol content and 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) assay for antiradical activity. In all samples, physicochemical parameters like moisture, reducing sugar, sucrose, ash, free acidity and water insoluble matter were also measured according to harmonized methods of the International Honey Commission (IHC). The results of physicochemical analysis showed that all the values, except for moisture of a small number of high altitude honey samples, are in good agreement with the current Nepalese standard. The total phenolic contents of honey, collected from high and low altitude, ranged from 154.87 to 41.90 mg gallic acid equivalent (GAE/100 g) respectively, at corresponding antiradical activity using DPPH• expressed as percent inhibition of 76.66% and 25.69%. The IC50 values of selected high altitude honey samples ranged from 56 to 72 mg/mL. The total antioxidant properties were correlated (P<0.01) between total phenol content and antiradical activity (r=0.992). The obtained results demonstrate that the Nepalese honey collected from high altitude region contained more antioxidants than honey of low altitude region. |
| format | Article |
| id | doaj-art-c593c6c9f95f43e19f6d0e171f5a7891 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-c593c6c9f95f43e19f6d0e171f5a78912025-02-02T15:29:20ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072015-06-01652879110.1515/pjfns-2015-0024pjfns-2015-0024Antioxidant Properties of Honey from Different Altitudes of Nepal HimalayasNeupane Bishnu Prasad0Malla Komal Prasad1Kaundinnyayana Atis2Poudel Prakash3Thapa Rashmi4Shrestha Sabina5School of Health and Allied Sciences, Pokhara University, Lekhnath-12, Kaski, PO Box No. 427, NepalSchool of Health and Allied Sciences, Pokhara University, Lekhnath-12, Kaski, PO Box No. 427, NepalSchool of Health and Allied Sciences, Pokhara University, Lekhnath-12, Kaski, PO Box No. 427, NepalSchool of Health and Allied Sciences, Pokhara University, Lekhnath-12, Kaski, PO Box No. 427, NepalSchool of Health and Allied Sciences, Pokhara University, Lekhnath-12, Kaski, PO Box No. 427, NepalFaculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju 690–756, Republic of KoreaTwenty two multifloral honey samples representing central western parts of Nepal were examined spectrophotometrically for their antioxidant properties and total phenol content. The modified Folin-Ciocalteu method was used to determine total phenol content and 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) assay for antiradical activity. In all samples, physicochemical parameters like moisture, reducing sugar, sucrose, ash, free acidity and water insoluble matter were also measured according to harmonized methods of the International Honey Commission (IHC). The results of physicochemical analysis showed that all the values, except for moisture of a small number of high altitude honey samples, are in good agreement with the current Nepalese standard. The total phenolic contents of honey, collected from high and low altitude, ranged from 154.87 to 41.90 mg gallic acid equivalent (GAE/100 g) respectively, at corresponding antiradical activity using DPPH• expressed as percent inhibition of 76.66% and 25.69%. The IC50 values of selected high altitude honey samples ranged from 56 to 72 mg/mL. The total antioxidant properties were correlated (P<0.01) between total phenol content and antiradical activity (r=0.992). The obtained results demonstrate that the Nepalese honey collected from high altitude region contained more antioxidants than honey of low altitude region.http://www.degruyter.com/view/j/pjfns.2015.65.issue-2/pjfns-2015-0024/pjfns-2015-0024.xml?format=INTNepalese honeyantioxidant activityphenolic contentphysicochemical parameters |
| spellingShingle | Neupane Bishnu Prasad Malla Komal Prasad Kaundinnyayana Atis Poudel Prakash Thapa Rashmi Shrestha Sabina Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas Polish Journal of Food and Nutrition Sciences Nepalese honey antioxidant activity phenolic content physicochemical parameters |
| title | Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas |
| title_full | Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas |
| title_fullStr | Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas |
| title_full_unstemmed | Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas |
| title_short | Antioxidant Properties of Honey from Different Altitudes of Nepal Himalayas |
| title_sort | antioxidant properties of honey from different altitudes of nepal himalayas |
| topic | Nepalese honey antioxidant activity phenolic content physicochemical parameters |
| url | http://www.degruyter.com/view/j/pjfns.2015.65.issue-2/pjfns-2015-0024/pjfns-2015-0024.xml?format=INT |
| work_keys_str_mv | AT neupanebishnuprasad antioxidantpropertiesofhoneyfromdifferentaltitudesofnepalhimalayas AT mallakomalprasad antioxidantpropertiesofhoneyfromdifferentaltitudesofnepalhimalayas AT kaundinnyayanaatis antioxidantpropertiesofhoneyfromdifferentaltitudesofnepalhimalayas AT poudelprakash antioxidantpropertiesofhoneyfromdifferentaltitudesofnepalhimalayas AT thaparashmi antioxidantpropertiesofhoneyfromdifferentaltitudesofnepalhimalayas AT shresthasabina antioxidantpropertiesofhoneyfromdifferentaltitudesofnepalhimalayas |