Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress
Bioactive compounds in plants, such as carrots, have been widely used for their benefits. In agriculture, their potential as biostimulants still needs to be investigated, especially for their possible antioxidant action in plants subjected to abiotic stresses, such as salinity. This work aimed to ev...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Seeds |
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| Online Access: | https://www.mdpi.com/2674-1024/4/2/17 |
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| author | Sheila Bigolin Teixeira Stefânia Nunes Pires Caroline Hernke Thiel Cristiane Deuner Diogo da Silva Moura Natalia da Silva Garcia Filipe Selau Carlos Fernanda Reolon de Souza Te Ming Tseng Sidnei Deuner |
| author_facet | Sheila Bigolin Teixeira Stefânia Nunes Pires Caroline Hernke Thiel Cristiane Deuner Diogo da Silva Moura Natalia da Silva Garcia Filipe Selau Carlos Fernanda Reolon de Souza Te Ming Tseng Sidnei Deuner |
| author_sort | Sheila Bigolin Teixeira |
| collection | DOAJ |
| description | Bioactive compounds in plants, such as carrots, have been widely used for their benefits. In agriculture, their potential as biostimulants still needs to be investigated, especially for their possible antioxidant action in plants subjected to abiotic stresses, such as salinity. This work aimed to evaluate the elicitor potential of carrot extract in alleviating salt stress in rice plants. This study aimed to evaluate the elicitor potential of carrot extract in alleviating saline stress in the rice cultivars BRS Querência and BRS 358. Aqueous extracts of carrot roots at concentrations of 0% (water), 25%, 50%, and 100% were used to soak rice seeds for 48 h, which were then subjected to different concentrations of NaCl (0, 25, 75, and 150 mM). To determine the effect of carrot extract as an elicitor under saline stress conditions, the following tests were conducted: germination, seedling length, dry mass, and oxidative stress through the activity of antioxidant enzymes, superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), peroxide content, and lipid peroxidation (hydrogen peroxide H<sub>2</sub>O<sub>2</sub> and malonaldehyde MDA). Carrot extract increased the germination rate and maintained germination even under increased salinity rates in both cultivars. The application of 25 mM NaCl also boosted germination rates, followed by a significant decrease due to increased salinity rates. Shoot and root lengths and dry mass parameters showed a linear decrease in response to increasing NaCl concentrations. The activities of superoxide dismutase (SOD), ascorbate peroxidase (APx), and catalase (CAT) enzymes tended to decrease as the concentration of carrot extract increased, whereas the opposite was observed with NaCl application. Based on the combined analysis of the evaluated parameters, carrot extract application under the tested conditions was efficient in mitigating oxidative stress caused by high salinity conditions. |
| format | Article |
| id | doaj-art-c59135cff6de49a4a6a0016dfd1b9980 |
| institution | Kabale University |
| issn | 2674-1024 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Seeds |
| spelling | doaj-art-c59135cff6de49a4a6a0016dfd1b99802025-08-20T03:29:48ZengMDPI AGSeeds2674-10242025-03-01421710.3390/seeds4020017Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt StressSheila Bigolin Teixeira0Stefânia Nunes Pires1Caroline Hernke Thiel2Cristiane Deuner3Diogo da Silva Moura4Natalia da Silva Garcia5Filipe Selau Carlos6Fernanda Reolon de Souza7Te Ming Tseng8Sidnei Deuner9Department of Botany, Biology Institute, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Botany, Biology Institute, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Botany, Biology Institute, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilFaculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Botany, Biology Institute, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Botany, Biology Institute, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilFaculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Plant and Soil Sciences, Mississippi State University, 32 Creeman St., Starkville, MS 39762, USADepartment of Plant and Soil Sciences, Mississippi State University, 32 Creeman St., Starkville, MS 39762, USADepartment of Botany, Biology Institute, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilBioactive compounds in plants, such as carrots, have been widely used for their benefits. In agriculture, their potential as biostimulants still needs to be investigated, especially for their possible antioxidant action in plants subjected to abiotic stresses, such as salinity. This work aimed to evaluate the elicitor potential of carrot extract in alleviating salt stress in rice plants. This study aimed to evaluate the elicitor potential of carrot extract in alleviating saline stress in the rice cultivars BRS Querência and BRS 358. Aqueous extracts of carrot roots at concentrations of 0% (water), 25%, 50%, and 100% were used to soak rice seeds for 48 h, which were then subjected to different concentrations of NaCl (0, 25, 75, and 150 mM). To determine the effect of carrot extract as an elicitor under saline stress conditions, the following tests were conducted: germination, seedling length, dry mass, and oxidative stress through the activity of antioxidant enzymes, superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), peroxide content, and lipid peroxidation (hydrogen peroxide H<sub>2</sub>O<sub>2</sub> and malonaldehyde MDA). Carrot extract increased the germination rate and maintained germination even under increased salinity rates in both cultivars. The application of 25 mM NaCl also boosted germination rates, followed by a significant decrease due to increased salinity rates. Shoot and root lengths and dry mass parameters showed a linear decrease in response to increasing NaCl concentrations. The activities of superoxide dismutase (SOD), ascorbate peroxidase (APx), and catalase (CAT) enzymes tended to decrease as the concentration of carrot extract increased, whereas the opposite was observed with NaCl application. Based on the combined analysis of the evaluated parameters, carrot extract application under the tested conditions was efficient in mitigating oxidative stress caused by high salinity conditions.https://www.mdpi.com/2674-1024/4/2/17<i>Oryza sativa</i> L.<i>Daucus carota</i>salinitygerminationgrowthoxidative stress |
| spellingShingle | Sheila Bigolin Teixeira Stefânia Nunes Pires Caroline Hernke Thiel Cristiane Deuner Diogo da Silva Moura Natalia da Silva Garcia Filipe Selau Carlos Fernanda Reolon de Souza Te Ming Tseng Sidnei Deuner Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress Seeds <i>Oryza sativa</i> L. <i>Daucus carota</i> salinity germination growth oxidative stress |
| title | Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress |
| title_full | Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress |
| title_fullStr | Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress |
| title_full_unstemmed | Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress |
| title_short | Biostimulant Effect and Antioxidant Responses of Carrot Extract and the Viability of Rice Seeds Under Salt Stress |
| title_sort | biostimulant effect and antioxidant responses of carrot extract and the viability of rice seeds under salt stress |
| topic | <i>Oryza sativa</i> L. <i>Daucus carota</i> salinity germination growth oxidative stress |
| url | https://www.mdpi.com/2674-1024/4/2/17 |
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