The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli
Background: Escherichia coli (E. coli) is a type of bacteria commonly found in the intestines of humans and animals that can be pathogenic and develop resistance to various antibiotics through different mechanisms, making them difficult to treat with standard therapies. So using alternative strategi...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Assam Journal of Internal Medicine |
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| Online Access: | https://journals.lww.com/10.4103/ajoim.ajoim_4_24 |
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| author | Haneen Waheed Ahmeed Falah Hasan Obayes Al-Khikani Khadeeja Kareem Khudair |
| author_facet | Haneen Waheed Ahmeed Falah Hasan Obayes Al-Khikani Khadeeja Kareem Khudair |
| author_sort | Haneen Waheed Ahmeed |
| collection | DOAJ |
| description | Background:
Escherichia coli (E. coli) is a type of bacteria commonly found in the intestines of humans and animals that can be pathogenic and develop resistance to various antibiotics through different mechanisms, making them difficult to treat with standard therapies. So using alternative strategies to treat E. coli is urgent, such as utilizing certain dyes that may be a promising branch in microbial therapy.
Materials and Methods:
The bacteria (E. coli) were isolated from the urinary tract and cultured in three media (blood agar, macConkey agar, and EMB agar), then they were incubated for 24-h at a temperature of 37°C to grow. Well diffusion method used to evaluate the antimicrobial activity of lactophenol, methyl red, and safranin stains. The antibiotic discs (Gentamicin) were used by the disc diffusion method. All the statistical analysis was done by using SPSS 26 software and the Excel app.Results:
The participants in this study were 66.67% women, while 33.33% were men, with significant differences (P < 0.05). Gentamicin showed high a rate of sensitivity (88.89%). All isolates of E. coli (100%) were sensitive to lactophenol; in contrast, all isolates (100%) were resistant to methyl red dye. E. coli isolates revealed 55.56% resistance to safranin.
Conclusions:
Lactophenol inhibition zone was 20.78 ± 5.40 mm, which revealed a great inhibition zone compared to gentamicin’s 17.22 ± 6.55 mm with no significant differences (P = 0.22). That means lactophenol is equal in activity to gentamicin to treat E. coli. |
| format | Article |
| id | doaj-art-c58ce84521454f1b96c20fe9846e6f1a |
| institution | OA Journals |
| issn | 2278-8239 2773-0166 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Assam Journal of Internal Medicine |
| spelling | doaj-art-c58ce84521454f1b96c20fe9846e6f1a2025-08-20T02:12:45ZengWolters Kluwer Medknow PublicationsAssam Journal of Internal Medicine2278-82392773-01662024-01-011413710.4103/ajoim.ajoim_4_24The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia ColiHaneen Waheed AhmeedFalah Hasan Obayes Al-KhikaniKhadeeja Kareem KhudairBackground: Escherichia coli (E. coli) is a type of bacteria commonly found in the intestines of humans and animals that can be pathogenic and develop resistance to various antibiotics through different mechanisms, making them difficult to treat with standard therapies. So using alternative strategies to treat E. coli is urgent, such as utilizing certain dyes that may be a promising branch in microbial therapy. Materials and Methods: The bacteria (E. coli) were isolated from the urinary tract and cultured in three media (blood agar, macConkey agar, and EMB agar), then they were incubated for 24-h at a temperature of 37°C to grow. Well diffusion method used to evaluate the antimicrobial activity of lactophenol, methyl red, and safranin stains. The antibiotic discs (Gentamicin) were used by the disc diffusion method. All the statistical analysis was done by using SPSS 26 software and the Excel app.Results: The participants in this study were 66.67% women, while 33.33% were men, with significant differences (P < 0.05). Gentamicin showed high a rate of sensitivity (88.89%). All isolates of E. coli (100%) were sensitive to lactophenol; in contrast, all isolates (100%) were resistant to methyl red dye. E. coli isolates revealed 55.56% resistance to safranin. Conclusions: Lactophenol inhibition zone was 20.78 ± 5.40 mm, which revealed a great inhibition zone compared to gentamicin’s 17.22 ± 6.55 mm with no significant differences (P = 0.22). That means lactophenol is equal in activity to gentamicin to treat E. coli.https://journals.lww.com/10.4103/ajoim.ajoim_4_24dyese. colilactophenolmethyl redsafraninurinary tract infection |
| spellingShingle | Haneen Waheed Ahmeed Falah Hasan Obayes Al-Khikani Khadeeja Kareem Khudair The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli Assam Journal of Internal Medicine dyes e. coli lactophenol methyl red safranin urinary tract infection |
| title | The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli |
| title_full | The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli |
| title_fullStr | The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli |
| title_full_unstemmed | The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli |
| title_short | The Antibacterial Activity of Lactophenol, Safranin, and Methyl Red Dyes Against Clinically Isolated Escherichia Coli |
| title_sort | antibacterial activity of lactophenol safranin and methyl red dyes against clinically isolated escherichia coli |
| topic | dyes e. coli lactophenol methyl red safranin urinary tract infection |
| url | https://journals.lww.com/10.4103/ajoim.ajoim_4_24 |
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