Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat
Study’s Excerpt: • Roots contained alkaloids, tannins, flavonoids, glycosides, and steroids. • Extract inhibited all multidrug-resistant isolates at 500 mg/mL. • Staphylococcus aureus showed highest inhibition (24.00 mm). • MIC ranged from 62.5 to 31.25 mg/mL; MBC from 125 to 62.5 mg/mL....
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Umaru Musa Yar'adua University, Katsina, Nigeria
2025-06-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1189 |
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| author | Yusuf Aliyu Munir Ahmad Sanusi Muhammad Maryam Hussaini Amina Darma Muhammad Abdulmajid Bashir Suwaiba Asababullah |
| author_facet | Yusuf Aliyu Munir Ahmad Sanusi Muhammad Maryam Hussaini Amina Darma Muhammad Abdulmajid Bashir Suwaiba Asababullah |
| author_sort | Yusuf Aliyu Munir |
| collection | DOAJ |
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Study’s Excerpt:
• Roots contained alkaloids, tannins, flavonoids, glycosides, and steroids.
• Extract inhibited all multidrug-resistant isolates at 500 mg/mL.
• Staphylococcus aureus showed highest inhibition (24.00 mm).
• MIC ranged from 62.5 to 31.25 mg/mL; MBC from 125 to 62.5 mg/mL.
• No significant difference in inhibition zones across bacteria tested.
Full Abstract:
The antibacterial activity of Cochlospermum tinctorium was determined in this study against Salmonella sp., Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, which were all antibiotic-resistant bacteria isolated from fresh chicken meat. The roots of Cochlospermum tinctorium were processed, and extraction was done by maceration. To determine the isolates' patterns of resistance and susceptibility to the antibiotics, antibiotic sensitivity testing was performed, while the disk diffusion method on Mueller Hinton Agar was used to assess the plant's antibacterial activity. The minimum Inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) were determined according to standard protocols. All statistical analyses were performed using R. The results showed all the bacterial isolates exhibited resistance to a number of widely used antibiotics: Septrin, Amoxicillin, Rocephin, Streptomycin, Sparfloxacin, Augmentin, Chloramphenicol, Ampicolox, Erythromycin. The phytochemical screening reveals the presence of alkaloids, tannins, cardiac glycosides, flavonoids, and steroids. Phytochemical screening revealed the presence of alkaloids, tannins, cardiac glycosides, flavonoids, and steroids. These compounds are known for their antimicrobial properties, suggesting that the extract contains bioactive substances that may contribute to its antibacterial potential. At a high concentration of 500 mg/mL, the extract of Cochlospermum tinctorium was effective in inhibiting all the isolates, with Staphylococcus aureus and Salmonella showing the highest zone of inhibition of 24.00mm and 23.00mm, respectively. The lowest inhibition was observed at 62.5mg/mL with Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus exhibiting the lowest inhibition at 4.00 mm, 6.00 mm, and 7.00 mm, respectively. The Minimum Inhibitory concentration (MIC) ranged from 62.5 to 31.25mg/mL for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Salmonella, while the minimum bacteriocidal concentration (MBC) was between 125 and 62.5mg/mL. Statistically, it shows no significant difference in the mean zone of inhibition of the plant extract against the bacterial isolates (F:0.22, F-crit: 3.24, P-value: 0.881, P>0.05). Indicating the extract may have a broad but uniform antibacterial effect. Further studies are recommended to explore its spectrum of activity, to identify the lead bioactive metabolite responsible for the antibacterial activity and its toxicological effect in biological organisms.
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| format | Article |
| id | doaj-art-c5895a14b1824c40ae2ddd8104cbfa73 |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-c5895a14b1824c40ae2ddd8104cbfa732025-08-20T03:40:21ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222025-06-0110310.47430/ujmr.25103.037Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken MeatYusuf Aliyu Munir0Ahmad Sanusi Muhammad1Maryam Hussaini2Amina Darma Muhammad3Abdulmajid Bashir4Suwaiba Asababullah5Department of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar'adua University, Katsina, NigeriaDepartment of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar'adua University, Katsina, NigeriaDepartment of Microbiology, College of Natural and Applied Sciences, Al-Qalam University Katsina, NigeriaDepartment of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar'adua University, Katsina, NigeriaDepartment of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar'adua University, Katsina, NigeriaDepartment of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar'adua University, Katsina, Nigeria Study’s Excerpt: • Roots contained alkaloids, tannins, flavonoids, glycosides, and steroids. • Extract inhibited all multidrug-resistant isolates at 500 mg/mL. • Staphylococcus aureus showed highest inhibition (24.00 mm). • MIC ranged from 62.5 to 31.25 mg/mL; MBC from 125 to 62.5 mg/mL. • No significant difference in inhibition zones across bacteria tested. Full Abstract: The antibacterial activity of Cochlospermum tinctorium was determined in this study against Salmonella sp., Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, which were all antibiotic-resistant bacteria isolated from fresh chicken meat. The roots of Cochlospermum tinctorium were processed, and extraction was done by maceration. To determine the isolates' patterns of resistance and susceptibility to the antibiotics, antibiotic sensitivity testing was performed, while the disk diffusion method on Mueller Hinton Agar was used to assess the plant's antibacterial activity. The minimum Inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) were determined according to standard protocols. All statistical analyses were performed using R. The results showed all the bacterial isolates exhibited resistance to a number of widely used antibiotics: Septrin, Amoxicillin, Rocephin, Streptomycin, Sparfloxacin, Augmentin, Chloramphenicol, Ampicolox, Erythromycin. The phytochemical screening reveals the presence of alkaloids, tannins, cardiac glycosides, flavonoids, and steroids. Phytochemical screening revealed the presence of alkaloids, tannins, cardiac glycosides, flavonoids, and steroids. These compounds are known for their antimicrobial properties, suggesting that the extract contains bioactive substances that may contribute to its antibacterial potential. At a high concentration of 500 mg/mL, the extract of Cochlospermum tinctorium was effective in inhibiting all the isolates, with Staphylococcus aureus and Salmonella showing the highest zone of inhibition of 24.00mm and 23.00mm, respectively. The lowest inhibition was observed at 62.5mg/mL with Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus exhibiting the lowest inhibition at 4.00 mm, 6.00 mm, and 7.00 mm, respectively. The Minimum Inhibitory concentration (MIC) ranged from 62.5 to 31.25mg/mL for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Salmonella, while the minimum bacteriocidal concentration (MBC) was between 125 and 62.5mg/mL. Statistically, it shows no significant difference in the mean zone of inhibition of the plant extract against the bacterial isolates (F:0.22, F-crit: 3.24, P-value: 0.881, P>0.05). Indicating the extract may have a broad but uniform antibacterial effect. Further studies are recommended to explore its spectrum of activity, to identify the lead bioactive metabolite responsible for the antibacterial activity and its toxicological effect in biological organisms. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1189Cochlospermum tinctoriumAntibiotic ResistanceAntibacterialPhytochemical ScreeningMinimum Inhibitory concentration |
| spellingShingle | Yusuf Aliyu Munir Ahmad Sanusi Muhammad Maryam Hussaini Amina Darma Muhammad Abdulmajid Bashir Suwaiba Asababullah Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat UMYU Journal of Microbiology Research Cochlospermum tinctorium Antibiotic Resistance Antibacterial Phytochemical Screening Minimum Inhibitory concentration |
| title | Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat |
| title_full | Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat |
| title_fullStr | Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat |
| title_full_unstemmed | Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat |
| title_short | Assessment of Antibacterial Potential of Cochlospermum tinctorium against Antibiotic-Resistant Bacteria Isolated from Raw Chicken Meat |
| title_sort | assessment of antibacterial potential of cochlospermum tinctorium against antibiotic resistant bacteria isolated from raw chicken meat |
| topic | Cochlospermum tinctorium Antibiotic Resistance Antibacterial Phytochemical Screening Minimum Inhibitory concentration |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1189 |
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