Optimization of the extraction process and combined determination of antibacterial ingredients of Li Peng
Li Peng is the Chaetomium of chestnut (involucre of plant chestnut), also called the involucre of chestnut, thorn shell of chestnut, or Sarg. of chestnut etc. In the areas rich in Chinese chestnut, large amounts of waste Li Peng was produced, besides a small amount of which can be used for firewood,...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2016-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.03.211 |
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| Summary: | Li Peng is the Chaetomium of chestnut (involucre of plant chestnut), also called the involucre of chestnut, thorn shell of chestnut, or Sarg. of chestnut etc. In the areas rich in Chinese chestnut, large amounts of waste Li Peng was produced, besides a small amount of which can be used for firewood, the rest will be discarded to rot or burned; however, it is not only a waste of resources, but also cause serious environmental pollution. Reports showed that Li Peng could be used as a raw material to cultivate chestnut mushrooms, but it was only an accessory ingredient, and the main ingredient was cotton seed shell of chestnut tree, so Li Peng has yet to be fully used. In some areas, the phenomenon of hyphae overgrowth occurred in chestnut mushrooms cultivated by Li Peng, the reason was due to its antibacterial effect.The purpose of this paper was to establish a simple and low-cost technique for extraction of antibacterial components from Li Peng. At the same time, a high sensitive, good repeatable and stable, simple and rapid method was used to determine the antibacterial ingredients of Li Peng.This paper used the single factor experiment and the orthogonal experiment optimization for the extraction process of antibacterial components in Li Peng. Furthermore, polysaccharide in the samples was precipitated by ethanol and the qualitative and quantitative combined determination of antibacterial components were carried out using reversed-phase high-performance liquid chromatography (RP-HPLC) method after protein removal.The results showed that the best way to extract the antibacterial composition of the 60-mesh fine slag in Li Peng was under 1∶40 material liquid ratio,70 W ultrasonic reflux 20 min. Using Lichrospher C<sub>18</sub> column (150 mm × 4.6 mm,5 μm), methanol-0.1% phosphoric acid solution as the mobile phase, the flow rate of 1 mL/min, the detection wavelength at 280 nm, column temperature of 30℃ with the gradient elution program, the gallic acid, protocatechuic acid, and quercetin content in Li Peng could be better determined. The quality concentrations of each component with peak area showed a good linear relationship (r>0.999) in the measurement range. The average sample recovery (n=9) rate were 98.9%,96.0%,97.1%. The relative standard deviation (RSD) were 2.36%,0.77%,4.73%.We optimized the extraction of antibacterial ingredients in Li Peng. By simple operation, low energy consumption and high extract efficiency method increased the feasibility of this technology, and provides theoretical basis of mass industrial production for the extraction of antibacterial ingredients in Li Peng. Under the optimum conditions, we can extract 88.2% antibacterial components from chestnut at one time. The antibacterial component of gallic acid, protocatechuic acid, quercetin content in Li Peng was 0.176 0 mg/g (RSD=0.23%),0.053 7 mg/g (RSD=0.16%),0.019 6 mg/g (RSD=0.21%), respectively. The RP-HPLC is a high sensitive, good repeatable and stable method, which is also simple, convenient and accurate. This paper enriched the reference basis for the combined determination of the antibacterial components of Li Peng. |
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| ISSN: | 1008-9209 2097-5155 |