Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study
With the rapid implementation of high-pressure processing in many sectors of the food industry, considerations associated with pressure-stressed microorganisms are emerging. Nisin was utilized in this study for controlling the proliferation of <i>Listeria monocytogenes</i> and <i>L...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/3/668 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849341694139432960 |
|---|---|
| author | Ranju Kafle Aliyar Cyrus Fouladkhah |
| author_facet | Ranju Kafle Aliyar Cyrus Fouladkhah |
| author_sort | Ranju Kafle |
| collection | DOAJ |
| description | With the rapid implementation of high-pressure processing in many sectors of the food industry, considerations associated with pressure-stressed microorganisms are emerging. Nisin was utilized in this study for controlling the proliferation of <i>Listeria monocytogenes</i> and <i>L. innocua</i> inoculated on cold-smoked trout during a 4-week refrigerated shelf-life trial. Wild-type and pressure-stressed phenotypes of <i>Listeria</i> were compared in this study. The pressure-stressed phenotypes were prepared by treating the surrogate strain and pathogen mixture at 103.4 MPa (15K PSI) for 20 min. <i>L. monocytogenes</i> multiplied extensively during the 4-week refrigerated trial and counts were increased (<i>p</i> < 0.05) from 3.68 ± 0.1 log CFU/g on the first week to 6.03 ± 0.1 log CFU/g. Both phenotypes and the surrogate microorganisms illustrated similar (<i>p</i> ≥ 0.05) multiplication trends. Unlike samples subjected to water treatment, nisin was effective (<i>p</i> < 0.05) in keeping the microbial counts lower compared with the controls, particularly earlier during the shelf-life trial. Our study illustrates that the selected surrogate microorganism has comparable sensitivity to nisin relative to <i>L. monocytogenes</i> and thus could be used interchangeably in future public health microbiology challenge studies with similar scope. Additionally, we observed that pressure-stressed <i>L. monocytogenes</i> has proliferation and sensitivity to nisin comparable to wild-type pathogen. |
| format | Article |
| id | doaj-art-c57cbb2be0d94cc2b746d353d4620b57 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-c57cbb2be0d94cc2b746d353d4620b572025-08-20T03:43:34ZengMDPI AGMicroorganisms2076-26072025-03-0113366810.3390/microorganisms13030668Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life StudyRanju Kafle0Aliyar Cyrus Fouladkhah1Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USAPublic Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USAWith the rapid implementation of high-pressure processing in many sectors of the food industry, considerations associated with pressure-stressed microorganisms are emerging. Nisin was utilized in this study for controlling the proliferation of <i>Listeria monocytogenes</i> and <i>L. innocua</i> inoculated on cold-smoked trout during a 4-week refrigerated shelf-life trial. Wild-type and pressure-stressed phenotypes of <i>Listeria</i> were compared in this study. The pressure-stressed phenotypes were prepared by treating the surrogate strain and pathogen mixture at 103.4 MPa (15K PSI) for 20 min. <i>L. monocytogenes</i> multiplied extensively during the 4-week refrigerated trial and counts were increased (<i>p</i> < 0.05) from 3.68 ± 0.1 log CFU/g on the first week to 6.03 ± 0.1 log CFU/g. Both phenotypes and the surrogate microorganisms illustrated similar (<i>p</i> ≥ 0.05) multiplication trends. Unlike samples subjected to water treatment, nisin was effective (<i>p</i> < 0.05) in keeping the microbial counts lower compared with the controls, particularly earlier during the shelf-life trial. Our study illustrates that the selected surrogate microorganism has comparable sensitivity to nisin relative to <i>L. monocytogenes</i> and thus could be used interchangeably in future public health microbiology challenge studies with similar scope. Additionally, we observed that pressure-stressed <i>L. monocytogenes</i> has proliferation and sensitivity to nisin comparable to wild-type pathogen.https://www.mdpi.com/2076-2607/13/3/668<i>Listeria monocytogenes</i> surrogatemesophilic background microbiotapressure-stressed <i>Listeria</i> spp. |
| spellingShingle | Ranju Kafle Aliyar Cyrus Fouladkhah Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study Microorganisms <i>Listeria monocytogenes</i> surrogate mesophilic background microbiota pressure-stressed <i>Listeria</i> spp. |
| title | Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study |
| title_full | Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study |
| title_fullStr | Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study |
| title_full_unstemmed | Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study |
| title_short | Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type <i>Listeria monocytogenes</i>, and <i>Listeria innocua</i> During a Real-Time Shelf-Life Study |
| title_sort | impact of nisin on proliferation of background microbiota pressure stressed and wild type i listeria monocytogenes i and i listeria innocua i during a real time shelf life study |
| topic | <i>Listeria monocytogenes</i> surrogate mesophilic background microbiota pressure-stressed <i>Listeria</i> spp. |
| url | https://www.mdpi.com/2076-2607/13/3/668 |
| work_keys_str_mv | AT ranjukafle impactofnisinonproliferationofbackgroundmicrobiotapressurestressedandwildtypeilisteriamonocytogenesiandilisteriainnocuaiduringarealtimeshelflifestudy AT aliyarcyrusfouladkhah impactofnisinonproliferationofbackgroundmicrobiotapressurestressedandwildtypeilisteriamonocytogenesiandilisteriainnocuaiduringarealtimeshelflifestudy |