Bacterial membrane vesicles restore gut anaerobiosis
Abstract Inflammation damages the epithelial cell barrier, allowing oxygen to leak into the lumen of the gut. Respiring E. coli and other Enterobacteriaceae produce proinflammatory lipopolysaccharide, exacerbating inflammatory bowel disease. Here we show that respiring membrane vesicles (MV) from E....
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-03-01
|
| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00676-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850057393187061760 |
|---|---|
| author | Norman Pitt Madeleine Morrissette Michael F. Gates Rachel Bargabos Megan Krumpoch Bryson Hawkins Kim Lewis |
| author_facet | Norman Pitt Madeleine Morrissette Michael F. Gates Rachel Bargabos Megan Krumpoch Bryson Hawkins Kim Lewis |
| author_sort | Norman Pitt |
| collection | DOAJ |
| description | Abstract Inflammation damages the epithelial cell barrier, allowing oxygen to leak into the lumen of the gut. Respiring E. coli and other Enterobacteriaceae produce proinflammatory lipopolysaccharide, exacerbating inflammatory bowel disease. Here we show that respiring membrane vesicles (MV) from E. coli ameliorate symptoms in a mouse model of gut inflammation. Membrane vesicle treatment diminished weight loss and limited shortening of the colon. Notably, oxygenation of the colonic epithelium was significantly decreased in animals receiving wild type MVs, but not MVs from an E. coli mutant lacking cytochromes. Metatranscriptomic analysis of the microbiome shows an increase in anaerobic Lactobacillaceae and a decrease in Enterobacteriaceae, as well as a general shift towards fermentation in MV-treated mice. This is accompanied by a decrease in proinflammatory TNF-α. We report that MVs may lead to the development of a novel type of a therapeutic for dysbiosis, and for treating IBD. |
| format | Article |
| id | doaj-art-c57ae19ec2d94b98976aa9507a57c0fe |
| institution | DOAJ |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-c57ae19ec2d94b98976aa9507a57c0fe2025-08-20T02:51:27ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-03-0111111110.1038/s41522-025-00676-zBacterial membrane vesicles restore gut anaerobiosisNorman Pitt0Madeleine Morrissette1Michael F. Gates2Rachel Bargabos3Megan Krumpoch4Bryson Hawkins5Kim Lewis6Antimicrobial Discovery Center, Department of Biology, Northeastern UniversityAntimicrobial Discovery Center, Department of Biology, Northeastern UniversityAntimicrobial Discovery Center, Department of Biology, Northeastern UniversityAntimicrobial Discovery Center, Department of Biology, Northeastern UniversityAntimicrobial Discovery Center, Department of Biology, Northeastern UniversityAntimicrobial Discovery Center, Department of Biology, Northeastern UniversityAntimicrobial Discovery Center, Department of Biology, Northeastern UniversityAbstract Inflammation damages the epithelial cell barrier, allowing oxygen to leak into the lumen of the gut. Respiring E. coli and other Enterobacteriaceae produce proinflammatory lipopolysaccharide, exacerbating inflammatory bowel disease. Here we show that respiring membrane vesicles (MV) from E. coli ameliorate symptoms in a mouse model of gut inflammation. Membrane vesicle treatment diminished weight loss and limited shortening of the colon. Notably, oxygenation of the colonic epithelium was significantly decreased in animals receiving wild type MVs, but not MVs from an E. coli mutant lacking cytochromes. Metatranscriptomic analysis of the microbiome shows an increase in anaerobic Lactobacillaceae and a decrease in Enterobacteriaceae, as well as a general shift towards fermentation in MV-treated mice. This is accompanied by a decrease in proinflammatory TNF-α. We report that MVs may lead to the development of a novel type of a therapeutic for dysbiosis, and for treating IBD.https://doi.org/10.1038/s41522-025-00676-z |
| spellingShingle | Norman Pitt Madeleine Morrissette Michael F. Gates Rachel Bargabos Megan Krumpoch Bryson Hawkins Kim Lewis Bacterial membrane vesicles restore gut anaerobiosis npj Biofilms and Microbiomes |
| title | Bacterial membrane vesicles restore gut anaerobiosis |
| title_full | Bacterial membrane vesicles restore gut anaerobiosis |
| title_fullStr | Bacterial membrane vesicles restore gut anaerobiosis |
| title_full_unstemmed | Bacterial membrane vesicles restore gut anaerobiosis |
| title_short | Bacterial membrane vesicles restore gut anaerobiosis |
| title_sort | bacterial membrane vesicles restore gut anaerobiosis |
| url | https://doi.org/10.1038/s41522-025-00676-z |
| work_keys_str_mv | AT normanpitt bacterialmembranevesiclesrestoregutanaerobiosis AT madeleinemorrissette bacterialmembranevesiclesrestoregutanaerobiosis AT michaelfgates bacterialmembranevesiclesrestoregutanaerobiosis AT rachelbargabos bacterialmembranevesiclesrestoregutanaerobiosis AT megankrumpoch bacterialmembranevesiclesrestoregutanaerobiosis AT brysonhawkins bacterialmembranevesiclesrestoregutanaerobiosis AT kimlewis bacterialmembranevesiclesrestoregutanaerobiosis |