Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice
Walnut peptide (WP) was chelated with calcium (Ca) to prepare WP-Ca chelate. The characterization, stability, and absorption properties of WP-Ca were investigated. Results showed that WP showed a calcium-binding capacity of 14.22 μg/mg, primarily through interactions with nitrogen and oxygen atoms....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325004508 |
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| author | Yaoxin Zhang Xuehang Wang Yuan Qi Pengyuan Pan Xiaoyi Li Yue Leng Ji Wang |
| author_facet | Yaoxin Zhang Xuehang Wang Yuan Qi Pengyuan Pan Xiaoyi Li Yue Leng Ji Wang |
| author_sort | Yaoxin Zhang |
| collection | DOAJ |
| description | Walnut peptide (WP) was chelated with calcium (Ca) to prepare WP-Ca chelate. The characterization, stability, and absorption properties of WP-Ca were investigated. Results showed that WP showed a calcium-binding capacity of 14.22 μg/mg, primarily through interactions with nitrogen and oxygen atoms. WP-Ca had good thermal stability and gastrointestinal digestive stability. In the flipped rat intestinal capsule model, the calcium absorption capacity of WP-Ca increased by approximately 3.75 times, and at the same time enhanced the calcium transport of the Caco-2 cell monolayer. Additionally, the results of animal experiments on calcium deficiency induced by a low-calcium diet indicated that WP-Ca (66.67 mg Ca/kg bw) supplementation effectively corrected calcium imbalance, significantly (P < 0.05) increased trabecular number and cortical thickness, and improved skeletal mechanical strength. Pearson's correlation analysis showed that the microbial genera of Bacteroidete, Akkermansia, Allobaculum, and Oscillospira were significantly (P < 0.05) associated with increased bone mass. Overall, these findings suggest that WP-Ca shows great potential in the development of new and highly efficient calcium supplements for osteoporosis prevention, nutritional fortification for special populations, and innovation in functional foods. |
| format | Article |
| id | doaj-art-c57319daf2a94307aa9411c1a9fd97c8 |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-c57319daf2a94307aa9411c1a9fd97c82025-08-20T03:14:02ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210207910.1016/j.jafr.2025.102079Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient miceYaoxin Zhang0Xuehang Wang1Yuan Qi2Pengyuan Pan3Xiaoyi Li4Yue Leng5Ji Wang6College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, PR ChinaCollege of Life Sciences, Changchun Sci-Tech University, Changchun, 130600, PR ChinaCollege of School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, PR ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, PR ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, PR ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, PR China; Corresponding author. No. 2888 Xincheng Street, College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, PR China.Walnut peptide (WP) was chelated with calcium (Ca) to prepare WP-Ca chelate. The characterization, stability, and absorption properties of WP-Ca were investigated. Results showed that WP showed a calcium-binding capacity of 14.22 μg/mg, primarily through interactions with nitrogen and oxygen atoms. WP-Ca had good thermal stability and gastrointestinal digestive stability. In the flipped rat intestinal capsule model, the calcium absorption capacity of WP-Ca increased by approximately 3.75 times, and at the same time enhanced the calcium transport of the Caco-2 cell monolayer. Additionally, the results of animal experiments on calcium deficiency induced by a low-calcium diet indicated that WP-Ca (66.67 mg Ca/kg bw) supplementation effectively corrected calcium imbalance, significantly (P < 0.05) increased trabecular number and cortical thickness, and improved skeletal mechanical strength. Pearson's correlation analysis showed that the microbial genera of Bacteroidete, Akkermansia, Allobaculum, and Oscillospira were significantly (P < 0.05) associated with increased bone mass. Overall, these findings suggest that WP-Ca shows great potential in the development of new and highly efficient calcium supplements for osteoporosis prevention, nutritional fortification for special populations, and innovation in functional foods.http://www.sciencedirect.com/science/article/pii/S2666154325004508Walnut peptide-calcium chelateCharacterizationStabilityCalcium absorptionGut microbiota |
| spellingShingle | Yaoxin Zhang Xuehang Wang Yuan Qi Pengyuan Pan Xiaoyi Li Yue Leng Ji Wang Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice Journal of Agriculture and Food Research Walnut peptide-calcium chelate Characterization Stability Calcium absorption Gut microbiota |
| title | Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice |
| title_full | Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice |
| title_fullStr | Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice |
| title_full_unstemmed | Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice |
| title_short | Preparation and characterization of a novel walnut peptide–calcium chelate and its role in promoting calcium absorption in calcium-deficient mice |
| title_sort | preparation and characterization of a novel walnut peptide calcium chelate and its role in promoting calcium absorption in calcium deficient mice |
| topic | Walnut peptide-calcium chelate Characterization Stability Calcium absorption Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325004508 |
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