Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma
Lung adenocarcinoma (LUAD), the most prevalent subtype of non-small cell lung cancer, is a significant cause of cancer-related mortality due to late-stage diagnoses and limited treatment options. Identifying effective biomarkers is crucial for improving prognosis and therapeutic strategies. miR-30a-...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825002092 |
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| author | Yunus Sahin Zekiye Altan Khandakar A.S.M. Saadat Masa-Aki Ikeda Ahmet Arslan |
| author_facet | Yunus Sahin Zekiye Altan Khandakar A.S.M. Saadat Masa-Aki Ikeda Ahmet Arslan |
| author_sort | Yunus Sahin |
| collection | DOAJ |
| description | Lung adenocarcinoma (LUAD), the most prevalent subtype of non-small cell lung cancer, is a significant cause of cancer-related mortality due to late-stage diagnoses and limited treatment options. Identifying effective biomarkers is crucial for improving prognosis and therapeutic strategies. miR-30a-5p has emerged as a potential tumor suppressor in LUAD, yet its exact role remains unclear. In this study, we analyzed three miRNA expression datasets from the GEO database and confirmed miR-30a downregulation in LUAD through TCGA and qRT-PCR validation. We identified MYBL2, TWF1, GALNT7, and PFN2 as oncogenic targets of miR-30a by integrating miRNA target predictions with gene expression data. Expression and survival analyses revealed an inverse relationship between miR-30a and these targets, with high expression levels correlating with poor patient outcomes. Functional enrichment analysis highlighted pathways in cell cycle regulation and actin cytoskeleton organization. Our findings underscore the significance of the miR-30a regulatory network in LUAD progression, positioning miR-30a and its targets as promising prognostic biomarkers and therapeutic targets. |
| format | Article |
| id | doaj-art-c550d44f0e0e4c6ca70cd9652ad244fb |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-c550d44f0e0e4c6ca70cd9652ad244fb2025-08-20T03:50:07ZengElsevierBiochemistry and Biophysics Reports2405-58082025-09-014310212210.1016/j.bbrep.2025.102122Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinomaYunus Sahin0Zekiye Altan1Khandakar A.S.M. Saadat2Masa-Aki Ikeda3Ahmet Arslan4Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey; Corresponding author.Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, TurkeyDepartment of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, TurkeyDepartment of Molecular Craniofacial Embryology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Medical Genetics, Faculty of Medicine, Research and Application Hospital, Tekirdag Namık Kemal University, Suleymanpasa, Tekirdag, TurkeyLung adenocarcinoma (LUAD), the most prevalent subtype of non-small cell lung cancer, is a significant cause of cancer-related mortality due to late-stage diagnoses and limited treatment options. Identifying effective biomarkers is crucial for improving prognosis and therapeutic strategies. miR-30a-5p has emerged as a potential tumor suppressor in LUAD, yet its exact role remains unclear. In this study, we analyzed three miRNA expression datasets from the GEO database and confirmed miR-30a downregulation in LUAD through TCGA and qRT-PCR validation. We identified MYBL2, TWF1, GALNT7, and PFN2 as oncogenic targets of miR-30a by integrating miRNA target predictions with gene expression data. Expression and survival analyses revealed an inverse relationship between miR-30a and these targets, with high expression levels correlating with poor patient outcomes. Functional enrichment analysis highlighted pathways in cell cycle regulation and actin cytoskeleton organization. Our findings underscore the significance of the miR-30a regulatory network in LUAD progression, positioning miR-30a and its targets as promising prognostic biomarkers and therapeutic targets.http://www.sciencedirect.com/science/article/pii/S2405580825002092Lung adenocarcinomamiR-30aPrognostic biomarkersMicroRNABioinformatics |
| spellingShingle | Yunus Sahin Zekiye Altan Khandakar A.S.M. Saadat Masa-Aki Ikeda Ahmet Arslan Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma Biochemistry and Biophysics Reports Lung adenocarcinoma miR-30a Prognostic biomarkers MicroRNA Bioinformatics |
| title | Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma |
| title_full | Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma |
| title_fullStr | Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma |
| title_full_unstemmed | Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma |
| title_short | Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma |
| title_sort | investigating mir 30a s tumor suppressor role and its targets mybl2 twf1 galnt7 pfn2 as prognostic biomarkers in lung adenocarcinoma |
| topic | Lung adenocarcinoma miR-30a Prognostic biomarkers MicroRNA Bioinformatics |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825002092 |
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