Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi
Male infertility affects millions worldwide, yet its underlying causes remain incompletely understood. Total round cell concentration (TRCC) in semen, particularly leukocytospermia, has been suggested as a biomarker of impaired sperm function. However, its relationship with sperm DNA fragmentation i...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-07-01
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| Series: | American Journal of Men's Health |
| Online Access: | https://doi.org/10.1177/15579883251359420 |
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| author | Evans Anokye Kumi Victor Boachie Owusu Peter Nyarko Coffie Ebenezer Kojo Addae Rex Kwadwo Mawuli Djokoto Kweku Bedu-Addo John Asiedu Larbi |
| author_facet | Evans Anokye Kumi Victor Boachie Owusu Peter Nyarko Coffie Ebenezer Kojo Addae Rex Kwadwo Mawuli Djokoto Kweku Bedu-Addo John Asiedu Larbi |
| author_sort | Evans Anokye Kumi |
| collection | DOAJ |
| description | Male infertility affects millions worldwide, yet its underlying causes remain incompletely understood. Total round cell concentration (TRCC) in semen, particularly leukocytospermia, has been suggested as a biomarker of impaired sperm function. However, its relationship with sperm DNA fragmentation index (DFI) remains unclear, particularly in sub-Saharan Africa, where research is scarce. This study examines the prevalence of elevated TRCC and its associations with semen parameters and sperm DNA fragmentation among men attending a fertility clinic in Kumasi, Ghana. A cross-sectional study was conducted among 227 men, with semen samples analyzed following WHO guidelines. Sperm concentration, motility, and morphology were assessed, while TRCC was quantified using a Neubauer hemocytometer and light microscopy. Sperm DNA fragmentation was determined using the Sperm Chromatin Structure Assay, and multivariate logistic regression models were employed to evaluate associations between TRCC and semen quality parameters. Elevated TRCC was detected in 19.4% of participants. Higher TRCC levels were significantly associated with lower odds of oligozoospermia (c OR = 0.30; 95% CI [0.20, 0.92]; p = .030), a relationship that remained significant after adjusting for confounders (a OR = 0.18; 95% CI [0.05, 0.67]; p = .010). However, TRCC was not significantly associated with asthenozoospermia ( p = .656) or teratozoospermia ( p = .592). Additionally, no correlation was observed between TRCC and sperm DFI ( r = .009, p = .958). It can therefore be concluded that the presence of round cells in semen does not influence sperm DNA integrity. |
| format | Article |
| id | doaj-art-c54042b880b14b40a75baf92c8d80a1f |
| institution | DOAJ |
| issn | 1557-9891 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | American Journal of Men's Health |
| spelling | doaj-art-c54042b880b14b40a75baf92c8d80a1f2025-08-20T03:09:44ZengSAGE PublishingAmerican Journal of Men's Health1557-98912025-07-011910.1177/15579883251359420Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in KumasiEvans Anokye Kumi0Victor Boachie Owusu1Peter Nyarko Coffie2Ebenezer Kojo Addae3Rex Kwadwo Mawuli Djokoto4Kweku Bedu-Addo5John Asiedu Larbi6Valley View University, Kumasi, GhanaOak Specialist Hospital, Kumasi, GhanaDepartment of Physiology, Accra College of Medicine, Accra, GhanaDepartment of Physiology, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Obstetrics and Gynaecology, Komfo Anokye Teaching Hospital, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Physiology, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaMale infertility affects millions worldwide, yet its underlying causes remain incompletely understood. Total round cell concentration (TRCC) in semen, particularly leukocytospermia, has been suggested as a biomarker of impaired sperm function. However, its relationship with sperm DNA fragmentation index (DFI) remains unclear, particularly in sub-Saharan Africa, where research is scarce. This study examines the prevalence of elevated TRCC and its associations with semen parameters and sperm DNA fragmentation among men attending a fertility clinic in Kumasi, Ghana. A cross-sectional study was conducted among 227 men, with semen samples analyzed following WHO guidelines. Sperm concentration, motility, and morphology were assessed, while TRCC was quantified using a Neubauer hemocytometer and light microscopy. Sperm DNA fragmentation was determined using the Sperm Chromatin Structure Assay, and multivariate logistic regression models were employed to evaluate associations between TRCC and semen quality parameters. Elevated TRCC was detected in 19.4% of participants. Higher TRCC levels were significantly associated with lower odds of oligozoospermia (c OR = 0.30; 95% CI [0.20, 0.92]; p = .030), a relationship that remained significant after adjusting for confounders (a OR = 0.18; 95% CI [0.05, 0.67]; p = .010). However, TRCC was not significantly associated with asthenozoospermia ( p = .656) or teratozoospermia ( p = .592). Additionally, no correlation was observed between TRCC and sperm DFI ( r = .009, p = .958). It can therefore be concluded that the presence of round cells in semen does not influence sperm DNA integrity.https://doi.org/10.1177/15579883251359420 |
| spellingShingle | Evans Anokye Kumi Victor Boachie Owusu Peter Nyarko Coffie Ebenezer Kojo Addae Rex Kwadwo Mawuli Djokoto Kweku Bedu-Addo John Asiedu Larbi Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi American Journal of Men's Health |
| title | Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi |
| title_full | Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi |
| title_fullStr | Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi |
| title_full_unstemmed | Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi |
| title_short | Total Round Cell Concentration in Semen and Its Association With Sperm DNA Fragmentation Index Among Ghanaian Males in Kumasi |
| title_sort | total round cell concentration in semen and its association with sperm dna fragmentation index among ghanaian males in kumasi |
| url | https://doi.org/10.1177/15579883251359420 |
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