Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites

Abstract In this study, we sequenced the complete mitochondrial genome of Theileria velifera and compared it with other Apicomplexan parasites. The mitochondrial genome of T. velifera is a linear monomer molecule spanning 6,125 bp, and it encodes 3 protein-coding genes (PCGs): cox1, cob, and cox3. B...

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Main Authors: Xing Yang, Shaobo Tang, Chaobo Du, Yuqing Chen, Zhi Luo, Miao Li, Shuang Liu, Mingna Duan, Dandan Jiang, Yujuan Shen, Quanfu Zhang, Chunhong Du
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-92939-0
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author Xing Yang
Shaobo Tang
Chaobo Du
Yuqing Chen
Zhi Luo
Miao Li
Shuang Liu
Mingna Duan
Dandan Jiang
Yujuan Shen
Quanfu Zhang
Chunhong Du
author_facet Xing Yang
Shaobo Tang
Chaobo Du
Yuqing Chen
Zhi Luo
Miao Li
Shuang Liu
Mingna Duan
Dandan Jiang
Yujuan Shen
Quanfu Zhang
Chunhong Du
author_sort Xing Yang
collection DOAJ
description Abstract In this study, we sequenced the complete mitochondrial genome of Theileria velifera and compared it with other Apicomplexan parasites. The mitochondrial genome of T. velifera is a linear monomer molecule spanning 6,125 bp, and it encodes 3 protein-coding genes (PCGs): cox1, cob, and cox3. Besides, it contains 5 large subunit (LSU) rRNA gene fragments and terminal inverted repeats (TIR) at both ends. Moreover, the mitochondrial genomes of most Apicomplexan parasites in this study are typically around 6,000 bp in length and are linear in structure, featuring three PCGs. The start codons observed in Thaleria spp. and Babesia spp. parasites predominantly include ATN, GTN, and TTN, while the end codons are mainly TAA, TAG, and TGA. Phylogenetic analysis showed that T. velifera was closely related to T. annulata, T. parva, T. taurotragi and T. lestoquardi. The complete mitochondrial genome sequence of T. velifera was examined and compared to other Apicomplexan parasites in this study, offering fresh perspectives on the evolution and phylogenetic connections among these parasites.
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spelling doaj-art-c516c8ff7381455ca7b5bbac7b203b862025-08-20T03:40:46ZengNature PortfolioScientific Reports2045-23222025-03-0115111210.1038/s41598-025-92939-0Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasitesXing Yang0Shaobo Tang1Chaobo Du2Yuqing Chen3Zhi Luo4Miao Li5Shuang Liu6Mingna Duan7Dandan Jiang8Yujuan Shen9Quanfu Zhang10Chunhong Du11National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Key Laboratory of Parasite and Vector Biology, Collaborating Centre for Tropical Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Health Commission of the People’s Republic of China, World Health OrganizationNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Key Laboratory of Parasite and Vector Biology, Collaborating Centre for Tropical Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Health Commission of the People’s Republic of China, World Health OrganizationYunan Institute of Eudemic Diseases Control and Prevention, Yunnan Provincial key Laboratory of Natural Epidemic Disease Prevention and Control TechnologyNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Key Laboratory of Parasite and Vector Biology, Collaborating Centre for Tropical Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Health Commission of the People’s Republic of China, World Health OrganizationYunan Institute of Eudemic Diseases Control and Prevention, Yunnan Provincial key Laboratory of Natural Epidemic Disease Prevention and Control TechnologyYunan Institute of Eudemic Diseases Control and Prevention, Yunnan Provincial key Laboratory of Natural Epidemic Disease Prevention and Control TechnologyIntegrated Laboratory of Pathogenic Biology, College of Preclinical Medicine, Dali UniversityIntegrated Laboratory of Pathogenic Biology, College of Preclinical Medicine, Dali UniversitySchool of Public Health, Dali UniversityNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Key Laboratory of Parasite and Vector Biology, Collaborating Centre for Tropical Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Health Commission of the People’s Republic of China, World Health OrganizationDepartment of Gastroenterology, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical CollegeYunan Institute of Eudemic Diseases Control and Prevention, Yunnan Provincial key Laboratory of Natural Epidemic Disease Prevention and Control TechnologyAbstract In this study, we sequenced the complete mitochondrial genome of Theileria velifera and compared it with other Apicomplexan parasites. The mitochondrial genome of T. velifera is a linear monomer molecule spanning 6,125 bp, and it encodes 3 protein-coding genes (PCGs): cox1, cob, and cox3. Besides, it contains 5 large subunit (LSU) rRNA gene fragments and terminal inverted repeats (TIR) at both ends. Moreover, the mitochondrial genomes of most Apicomplexan parasites in this study are typically around 6,000 bp in length and are linear in structure, featuring three PCGs. The start codons observed in Thaleria spp. and Babesia spp. parasites predominantly include ATN, GTN, and TTN, while the end codons are mainly TAA, TAG, and TGA. Phylogenetic analysis showed that T. velifera was closely related to T. annulata, T. parva, T. taurotragi and T. lestoquardi. The complete mitochondrial genome sequence of T. velifera was examined and compared to other Apicomplexan parasites in this study, offering fresh perspectives on the evolution and phylogenetic connections among these parasites.https://doi.org/10.1038/s41598-025-92939-0Theileria veliferaApicomplexaMitochondrial genomePhylogenetic analysisEvolutionary relationship
spellingShingle Xing Yang
Shaobo Tang
Chaobo Du
Yuqing Chen
Zhi Luo
Miao Li
Shuang Liu
Mingna Duan
Dandan Jiang
Yujuan Shen
Quanfu Zhang
Chunhong Du
Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites
Scientific Reports
Theileria velifera
Apicomplexa
Mitochondrial genome
Phylogenetic analysis
Evolutionary relationship
title Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites
title_full Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites
title_fullStr Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites
title_full_unstemmed Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites
title_short Insights into the mitochondrial genome structure and phylogenetic placement of Theileria velifera in comparison to other apicomplexan parasites
title_sort insights into the mitochondrial genome structure and phylogenetic placement of theileria velifera in comparison to other apicomplexan parasites
topic Theileria velifera
Apicomplexa
Mitochondrial genome
Phylogenetic analysis
Evolutionary relationship
url https://doi.org/10.1038/s41598-025-92939-0
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