Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation
Objective: Human interleukin-10 (IL-10) is a dimeric and pleiotropic cytokine that plays a crucial role in cellular immunoregulatory responses. As IL-10 binds to its receptors, IL-10Ra and IL-10Rb, it will suppress or induce the downstream cellular immune responses to protect from diseases. Material...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Tzu Chi Medical Journal |
| Subjects: | |
| Online Access: | http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2020;volume=32;issue=3;spage=245;epage=253;aulast=Chang |
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| _version_ | 1849323440247406592 |
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| author | Chun-Chun Chang Cheng-Der Liu Sheng-Feng Pan Wei-Han Huang Chih-Wen Peng Hao-Jen Hsu |
| author_facet | Chun-Chun Chang Cheng-Der Liu Sheng-Feng Pan Wei-Han Huang Chih-Wen Peng Hao-Jen Hsu |
| author_sort | Chun-Chun Chang |
| collection | DOAJ |
| description | Objective: Human interleukin-10 (IL-10) is a dimeric and pleiotropic cytokine that plays a crucial role in cellular immunoregulatory responses. As IL-10 binds to its receptors, IL-10Ra and IL-10Rb, it will suppress or induce the downstream cellular immune responses to protect from diseases. Materials and Methods: In this study, a potential peptide derived from IL-10 based on molecular docking and structural analysis was designed and validated by a series of cell assays to block IL-10 binding to receptor IL-10Ra for the inhibition of cell growth. Results: The simulation results indicate that the designed peptide IL10NM25 bound to receptor IL-10Ra is dominated by electrostatic interactions, whereas van der Waals (VDW) and hydrophobic interactions are minor. The cell experiments showed that IL10NM25 specifically binds to receptor IL-10Ra on the cell surface of two B-lineage cell lines, B lymphoma derived (BJAB), and lymphoblastoid cell line, whereas the mutant and scramble peptides are not able to suppress the binding of IL-10 to receptor IL-10Ra, consistent with the molecular simulation predictions. Conclusion: This study demonstrates that structure-based peptide design can be effective in the development of peptide drug discovery. |
| format | Article |
| id | doaj-art-c51033471ebe49fea10d509f138f25dc |
| institution | Kabale University |
| issn | 1016-3190 2223-8956 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Tzu Chi Medical Journal |
| spelling | doaj-art-c51033471ebe49fea10d509f138f25dc2025-08-20T03:49:03ZengWolters Kluwer Medknow PublicationsTzu Chi Medical Journal1016-31902223-89562020-01-0132324525310.4103/tcmj.tcmj_237_19Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferationChun-Chun ChangCheng-Der LiuSheng-Feng PanWei-Han HuangChih-Wen PengHao-Jen HsuObjective: Human interleukin-10 (IL-10) is a dimeric and pleiotropic cytokine that plays a crucial role in cellular immunoregulatory responses. As IL-10 binds to its receptors, IL-10Ra and IL-10Rb, it will suppress or induce the downstream cellular immune responses to protect from diseases. Materials and Methods: In this study, a potential peptide derived from IL-10 based on molecular docking and structural analysis was designed and validated by a series of cell assays to block IL-10 binding to receptor IL-10Ra for the inhibition of cell growth. Results: The simulation results indicate that the designed peptide IL10NM25 bound to receptor IL-10Ra is dominated by electrostatic interactions, whereas van der Waals (VDW) and hydrophobic interactions are minor. The cell experiments showed that IL10NM25 specifically binds to receptor IL-10Ra on the cell surface of two B-lineage cell lines, B lymphoma derived (BJAB), and lymphoblastoid cell line, whereas the mutant and scramble peptides are not able to suppress the binding of IL-10 to receptor IL-10Ra, consistent with the molecular simulation predictions. Conclusion: This study demonstrates that structure-based peptide design can be effective in the development of peptide drug discovery.http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2020;volume=32;issue=3;spage=245;epage=253;aulast=Changcytokineinterleukin-10molecular dockingpeptide design |
| spellingShingle | Chun-Chun Chang Cheng-Der Liu Sheng-Feng Pan Wei-Han Huang Chih-Wen Peng Hao-Jen Hsu Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation Tzu Chi Medical Journal cytokine interleukin-10 molecular docking peptide design |
| title | Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation |
| title_full | Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation |
| title_fullStr | Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation |
| title_full_unstemmed | Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation |
| title_short | Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferation |
| title_sort | targeting of interleukin 10 receptor by a potential human interleukin 10 peptide efficiently blocks interleukin 10 pathway dependent cell proliferation |
| topic | cytokine interleukin-10 molecular docking peptide design |
| url | http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2020;volume=32;issue=3;spage=245;epage=253;aulast=Chang |
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