Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition

Black elderberry (<i>Sambucus nigra</i> L.) flowers are rich in polyphenolic compounds, including chlorogenic acid and quercetin derivatives, which are known for their health benefits, particularly their antioxidant and antidiabetic properties. This study aimed to optimize the extraction...

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Main Authors: Elżbieta Studzińska-Sroka, Magdalena Paczkowska-Walendowska, Justyna Kledzik, Agnieszka Galanty, Anna Gościniak, Piotr Szulc, Katarzyna Korybalska, Judyta Cielecka-Piontek
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/23/5775
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author Elżbieta Studzińska-Sroka
Magdalena Paczkowska-Walendowska
Justyna Kledzik
Agnieszka Galanty
Anna Gościniak
Piotr Szulc
Katarzyna Korybalska
Judyta Cielecka-Piontek
author_facet Elżbieta Studzińska-Sroka
Magdalena Paczkowska-Walendowska
Justyna Kledzik
Agnieszka Galanty
Anna Gościniak
Piotr Szulc
Katarzyna Korybalska
Judyta Cielecka-Piontek
author_sort Elżbieta Studzińska-Sroka
collection DOAJ
description Black elderberry (<i>Sambucus nigra</i> L.) flowers are rich in polyphenolic compounds, including chlorogenic acid and quercetin derivatives, which are known for their health benefits, particularly their antioxidant and antidiabetic properties. This study aimed to optimize the extraction conditions using the Box–Behnken model to maximize polyphenol yields from different elderberry flower cultivars and to evaluate their potential for antidiabetic action. The extracts were analyzed for their phytochemical content and assessed for enzyme inhibition, specifically targeting enzymes critical in carbohydrate digestion and glucose regulation. The anti-inflammatory activity was also assessed. Results indicated that the Black Beauty, Obelisk, and Haschberg cultivars demonstrated significant inhibition of α-glucosidase, with a high inhibitory potential against α-amylase enzymes for the Obelisk cultivar. Additionally, high chlorogenic acid content was strongly correlated with enzyme inhibition and antioxidant activity, suggesting its substantial role in glucose regulation. This study underscores the potential of elderberry flower extracts, particularly those rich in chlorogenic acid, as natural agents for managing blood glucose levels, warranting further exploration of their use in antidiabetic applications.
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spelling doaj-art-c50cc3d4702143fcb2791e1d3e51e0eb2025-08-20T01:55:31ZengMDPI AGMolecules1420-30492024-12-012923577510.3390/molecules29235775Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme InhibitionElżbieta Studzińska-Sroka0Magdalena Paczkowska-Walendowska1Justyna Kledzik2Agnieszka Galanty3Anna Gościniak4Piotr Szulc5Katarzyna Korybalska6Judyta Cielecka-Piontek7Department of Pharmacognosy and Biomaterials, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, PolandDepartment of Pharmacognosy and Biomaterials, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, PolandDepartment of Pharmacognosy and Biomaterials, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, PolandDepartment of Pharmacognosy and Biomaterials, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, PolandDepartment of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznan, PolandDepartment of Pathophysiology, Poznań University of Medical Sciences, Rokietnicka 8, 60-806 Poznan, PolandDepartment of Pharmacognosy and Biomaterials, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, PolandBlack elderberry (<i>Sambucus nigra</i> L.) flowers are rich in polyphenolic compounds, including chlorogenic acid and quercetin derivatives, which are known for their health benefits, particularly their antioxidant and antidiabetic properties. This study aimed to optimize the extraction conditions using the Box–Behnken model to maximize polyphenol yields from different elderberry flower cultivars and to evaluate their potential for antidiabetic action. The extracts were analyzed for their phytochemical content and assessed for enzyme inhibition, specifically targeting enzymes critical in carbohydrate digestion and glucose regulation. The anti-inflammatory activity was also assessed. Results indicated that the Black Beauty, Obelisk, and Haschberg cultivars demonstrated significant inhibition of α-glucosidase, with a high inhibitory potential against α-amylase enzymes for the Obelisk cultivar. Additionally, high chlorogenic acid content was strongly correlated with enzyme inhibition and antioxidant activity, suggesting its substantial role in glucose regulation. This study underscores the potential of elderberry flower extracts, particularly those rich in chlorogenic acid, as natural agents for managing blood glucose levels, warranting further exploration of their use in antidiabetic applications.https://www.mdpi.com/1420-3049/29/23/5775black elderberry<i>Sambucus nigra</i>wild elderberrypolyphenolschlorogenic acidantioxidant activity
spellingShingle Elżbieta Studzińska-Sroka
Magdalena Paczkowska-Walendowska
Justyna Kledzik
Agnieszka Galanty
Anna Gościniak
Piotr Szulc
Katarzyna Korybalska
Judyta Cielecka-Piontek
Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
Molecules
black elderberry
<i>Sambucus nigra</i>
wild elderberry
polyphenols
chlorogenic acid
antioxidant activity
title Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
title_full Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
title_fullStr Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
title_full_unstemmed Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
title_short Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
title_sort antidiabetic potential of black elderberry cultivars flower extracts phytochemical profile and enzyme inhibition
topic black elderberry
<i>Sambucus nigra</i>
wild elderberry
polyphenols
chlorogenic acid
antioxidant activity
url https://www.mdpi.com/1420-3049/29/23/5775
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