Inhibition of Akt Attenuates RPO-Induced Cardioprotection
Previous studies have shown that red palm oil (RPO) supplementation protected rat hearts against ischaemia-reperfusion injury. Evidence from these studies suggested that Akt may be partly responsible for the observed protection. The aim of the current study was therefore to prove or refute the invol...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/392457 |
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| author | Emma Katengua-Thamahane Anna-Mart Engelbrecht Adriaan J. Esterhuyse Jacques Van Rooyen |
| author_facet | Emma Katengua-Thamahane Anna-Mart Engelbrecht Adriaan J. Esterhuyse Jacques Van Rooyen |
| author_sort | Emma Katengua-Thamahane |
| collection | DOAJ |
| description | Previous studies have shown that red palm oil (RPO) supplementation protected rat hearts against ischaemia-reperfusion injury. Evidence from these studies suggested that Akt may be partly responsible for the observed protection. The aim of the current study was therefore to prove or refute the involvement of Akt in the RPO-induced cardioprotection by administration of a specific Akt inhibitor (A6730). Male Wistar rats were randomly divided into 2 groups: a control group receiving standard rat chow and an experimental group receiving standard rat chow plus 2 mL RPO for six weeks. Hearts were excised and mounted on the Langendorff perfusion system. Functional recovery was documented. A different set of hearts were freeze-clamped to assess total and phosphorylation status of Akt. Another set of hearts were subjected to the same perfusion conditions with addition of A6730. Hearts from this protocol were freeze-clamped and assessed for total and phospho-Akt. RPO improved functional recovery which was associated with increased phosphorylation of Akt on Ser473 and Thr308 residues. Blockade of Akt phosphorylation caused poor functional recovery. For the first time, these results prove that Akt plays an important role in the RPO-induced cardioprotection. |
| format | Article |
| id | doaj-art-c506e830fb954876bbb06726690b6dbe |
| institution | DOAJ |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-c506e830fb954876bbb06726690b6dbe2025-08-20T03:23:04ZengWileyCardiology Research and Practice2090-80162090-05972012-01-01201210.1155/2012/392457392457Inhibition of Akt Attenuates RPO-Induced CardioprotectionEmma Katengua-Thamahane0Anna-Mart Engelbrecht1Adriaan J. Esterhuyse2Jacques Van Rooyen3Experimental Anti-oxidant Research Division, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Symphony Road, Western Cape, Bellville 7535, South AfricaDepartment of Physiological Sciences, University of Stellenbosch, Stellenbosch 7600, South AfricaExperimental Anti-oxidant Research Division, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Symphony Road, Western Cape, Bellville 7535, South AfricaExperimental Anti-oxidant Research Division, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Symphony Road, Western Cape, Bellville 7535, South AfricaPrevious studies have shown that red palm oil (RPO) supplementation protected rat hearts against ischaemia-reperfusion injury. Evidence from these studies suggested that Akt may be partly responsible for the observed protection. The aim of the current study was therefore to prove or refute the involvement of Akt in the RPO-induced cardioprotection by administration of a specific Akt inhibitor (A6730). Male Wistar rats were randomly divided into 2 groups: a control group receiving standard rat chow and an experimental group receiving standard rat chow plus 2 mL RPO for six weeks. Hearts were excised and mounted on the Langendorff perfusion system. Functional recovery was documented. A different set of hearts were freeze-clamped to assess total and phosphorylation status of Akt. Another set of hearts were subjected to the same perfusion conditions with addition of A6730. Hearts from this protocol were freeze-clamped and assessed for total and phospho-Akt. RPO improved functional recovery which was associated with increased phosphorylation of Akt on Ser473 and Thr308 residues. Blockade of Akt phosphorylation caused poor functional recovery. For the first time, these results prove that Akt plays an important role in the RPO-induced cardioprotection.http://dx.doi.org/10.1155/2012/392457 |
| spellingShingle | Emma Katengua-Thamahane Anna-Mart Engelbrecht Adriaan J. Esterhuyse Jacques Van Rooyen Inhibition of Akt Attenuates RPO-Induced Cardioprotection Cardiology Research and Practice |
| title | Inhibition of Akt Attenuates RPO-Induced Cardioprotection |
| title_full | Inhibition of Akt Attenuates RPO-Induced Cardioprotection |
| title_fullStr | Inhibition of Akt Attenuates RPO-Induced Cardioprotection |
| title_full_unstemmed | Inhibition of Akt Attenuates RPO-Induced Cardioprotection |
| title_short | Inhibition of Akt Attenuates RPO-Induced Cardioprotection |
| title_sort | inhibition of akt attenuates rpo induced cardioprotection |
| url | http://dx.doi.org/10.1155/2012/392457 |
| work_keys_str_mv | AT emmakatenguathamahane inhibitionofaktattenuatesrpoinducedcardioprotection AT annamartengelbrecht inhibitionofaktattenuatesrpoinducedcardioprotection AT adriaanjesterhuyse inhibitionofaktattenuatesrpoinducedcardioprotection AT jacquesvanrooyen inhibitionofaktattenuatesrpoinducedcardioprotection |