Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology
<i>Beauveria bassiana</i> has distinct nutrient requirements depending on the growth stage, with the mycelial growth stage being particularly nutrient-intense. When nutrients are exhausted, <i>B. bassiana</i> produces numerous spores that re-germinate and release visible hyph...
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2024-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/11/587 |
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| author | Hanxiao Lou Qizheng Luo Qingqing Guo Runshen Su Jianfeng Liu Hongli He Yunqing Cheng |
| author_facet | Hanxiao Lou Qizheng Luo Qingqing Guo Runshen Su Jianfeng Liu Hongli He Yunqing Cheng |
| author_sort | Hanxiao Lou |
| collection | DOAJ |
| description | <i>Beauveria bassiana</i> has distinct nutrient requirements depending on the growth stage, with the mycelial growth stage being particularly nutrient-intense. When nutrients are exhausted, <i>B. bassiana</i> produces numerous spores that re-germinate and release visible hyphae under suitable environmental conditions. This study aimed to use low-cost agricultural by-products for developing an optimized sporulation medium. Single-factor experiments revealed that potato, wheat bran, and wheat had the greatest influence on sporulation after 14 d of culture. Wheat, wheat bran, and corncob had the strongest effect on spore production. Using a mathematical model and Box–Behnken design, the maximum predicted sporulation using the stock medium was 5.55 × 10<sup>6</sup>/mL. The optimized formula comprised 54.82 g potato, 77.74 g wheat bran, and 101.48 g wheat. The actual stock medium produced a spore yield of 5.52 × 10<sup>6</sup>/mL. Similarly, the maximum predicted sporulation was 15.66 × 10<sup>6</sup>/mL for the culture medium. The optimized formula comprised 50.81 g wheat, 99.6 g wheat bran, and 112.59 g corncob, with a spore yield of 15.69 × 10<sup>6</sup>/mL. The high consistency between predicted and experimental values indicated that our model can accurately determine the effect of medium on sporulation. |
| format | Article |
| id | doaj-art-c501945af5d0456b809407e36fc1c2f0 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-c501945af5d0456b809407e36fc1c2f02025-08-20T02:08:11ZengMDPI AGFermentation2311-56372024-11-01101158710.3390/fermentation10110587Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface MethodologyHanxiao Lou0Qizheng Luo1Qingqing Guo2Runshen Su3Jianfeng Liu4Hongli He5Yunqing Cheng6Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaProvincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaProvincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaProvincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaProvincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaProvincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaProvincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, China<i>Beauveria bassiana</i> has distinct nutrient requirements depending on the growth stage, with the mycelial growth stage being particularly nutrient-intense. When nutrients are exhausted, <i>B. bassiana</i> produces numerous spores that re-germinate and release visible hyphae under suitable environmental conditions. This study aimed to use low-cost agricultural by-products for developing an optimized sporulation medium. Single-factor experiments revealed that potato, wheat bran, and wheat had the greatest influence on sporulation after 14 d of culture. Wheat, wheat bran, and corncob had the strongest effect on spore production. Using a mathematical model and Box–Behnken design, the maximum predicted sporulation using the stock medium was 5.55 × 10<sup>6</sup>/mL. The optimized formula comprised 54.82 g potato, 77.74 g wheat bran, and 101.48 g wheat. The actual stock medium produced a spore yield of 5.52 × 10<sup>6</sup>/mL. Similarly, the maximum predicted sporulation was 15.66 × 10<sup>6</sup>/mL for the culture medium. The optimized formula comprised 50.81 g wheat, 99.6 g wheat bran, and 112.59 g corncob, with a spore yield of 15.69 × 10<sup>6</sup>/mL. The high consistency between predicted and experimental values indicated that our model can accurately determine the effect of medium on sporulation.https://www.mdpi.com/2311-5637/10/11/587<i>Beauveria bassiana</i>hazelnuthazel weevilsporulation |
| spellingShingle | Hanxiao Lou Qizheng Luo Qingqing Guo Runshen Su Jianfeng Liu Hongli He Yunqing Cheng Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology Fermentation <i>Beauveria bassiana</i> hazelnut hazel weevil sporulation |
| title | Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology |
| title_full | Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology |
| title_fullStr | Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology |
| title_full_unstemmed | Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology |
| title_short | Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology |
| title_sort | optimization of the culture medium of i beauveria bassiana i and spore yield using response surface methodology |
| topic | <i>Beauveria bassiana</i> hazelnut hazel weevil sporulation |
| url | https://www.mdpi.com/2311-5637/10/11/587 |
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