Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater

<i>Rhodopseudomonas</i> is recognized for its versatile metabolic capabilities that enable it to effectively degrade pollutants and survive various environmental stresses. In this study, we conducted a genome analysis of <i>Rhodopseudomonas</i> sp. P1 to investigate its genet...

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Main Authors: Chollachai Klaysubun, Nattarika Chaichana, Sirikan Suwannasin, Kamonnut Singkhamanan, Thunchanok Yaikhan, Duangporn Kantachote, Rattanaruji Pomwised, Monwadee Wonglapsuwan, Komwit Surachat
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Language:English
Published: MDPI AG 2025-05-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/15/5/754
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author Chollachai Klaysubun
Nattarika Chaichana
Sirikan Suwannasin
Kamonnut Singkhamanan
Thunchanok Yaikhan
Duangporn Kantachote
Rattanaruji Pomwised
Monwadee Wonglapsuwan
Komwit Surachat
author_facet Chollachai Klaysubun
Nattarika Chaichana
Sirikan Suwannasin
Kamonnut Singkhamanan
Thunchanok Yaikhan
Duangporn Kantachote
Rattanaruji Pomwised
Monwadee Wonglapsuwan
Komwit Surachat
author_sort Chollachai Klaysubun
collection DOAJ
description <i>Rhodopseudomonas</i> is recognized for its versatile metabolic capabilities that enable it to effectively degrade pollutants and survive various environmental stresses. In this study, we conducted a genome analysis of <i>Rhodopseudomonas</i> sp. P1 to investigate its genetic potential for wastewater treatment processes. Phylogenetic and genome-relatedness analyses confirmed that strain P1 is genetically distinct from other species within the <i>Rhodopseudomonas</i> genus, establishing it as a novel species. The genome sequences obtained and analyzed focused on genes related to carbon and nutrient removal, photosynthetic capabilities, nitrate and nitrite reduction, and the biodegradation of common wastewater pollutants. The identification of wastewater treatment-related genes followed an extensive review of the existing literature that helped in selecting genes involved in various wastewater treatment mechanisms. The genome of <i>Rhodopseudomonas</i> sp. P1 contains a diverse array of genes involved in carbon and nutrient cycling, pollutant biodegradation, and metal resistance, all of which are crucial for its survival in the complex wastewater environment. Specifically, the strain contains genes responsible for the denitrification, nitrogen fixation, sulfur cycling, and detoxification of toxic metals such as copper and arsenic. These findings highlight the potential application of <i>Rhodopseudomonas</i> sp. P1 in wastewater treatment, particularly in environments contaminated with organic pollutants and heavy metals. However, while the genomic features indicate significant promise, the practical implementation of <i>Rhodopseudomonas</i> sp. P1 in real-world wastewater treatment systems will require further investigation, optimization, and validation to fully harness its potential for sustainable and efficient wastewater treatment.
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spelling doaj-art-c4e9a34214ec4f40a847daf9d9d2abbb2025-08-20T01:56:31ZengMDPI AGLife2075-17292025-05-0115575410.3390/life15050754Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet WastewaterChollachai Klaysubun0Nattarika Chaichana1Sirikan Suwannasin2Kamonnut Singkhamanan3Thunchanok Yaikhan4Duangporn Kantachote5Rattanaruji Pomwised6Monwadee Wonglapsuwan7Komwit Surachat8Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand<i>Rhodopseudomonas</i> is recognized for its versatile metabolic capabilities that enable it to effectively degrade pollutants and survive various environmental stresses. In this study, we conducted a genome analysis of <i>Rhodopseudomonas</i> sp. P1 to investigate its genetic potential for wastewater treatment processes. Phylogenetic and genome-relatedness analyses confirmed that strain P1 is genetically distinct from other species within the <i>Rhodopseudomonas</i> genus, establishing it as a novel species. The genome sequences obtained and analyzed focused on genes related to carbon and nutrient removal, photosynthetic capabilities, nitrate and nitrite reduction, and the biodegradation of common wastewater pollutants. The identification of wastewater treatment-related genes followed an extensive review of the existing literature that helped in selecting genes involved in various wastewater treatment mechanisms. The genome of <i>Rhodopseudomonas</i> sp. P1 contains a diverse array of genes involved in carbon and nutrient cycling, pollutant biodegradation, and metal resistance, all of which are crucial for its survival in the complex wastewater environment. Specifically, the strain contains genes responsible for the denitrification, nitrogen fixation, sulfur cycling, and detoxification of toxic metals such as copper and arsenic. These findings highlight the potential application of <i>Rhodopseudomonas</i> sp. P1 in wastewater treatment, particularly in environments contaminated with organic pollutants and heavy metals. However, while the genomic features indicate significant promise, the practical implementation of <i>Rhodopseudomonas</i> sp. P1 in real-world wastewater treatment systems will require further investigation, optimization, and validation to fully harness its potential for sustainable and efficient wastewater treatment.https://www.mdpi.com/2075-1729/15/5/754purple non-sulfur bacteria<i>Rhodopseudomonas</i>wastewater treatmentgenomebioinformatics
spellingShingle Chollachai Klaysubun
Nattarika Chaichana
Sirikan Suwannasin
Kamonnut Singkhamanan
Thunchanok Yaikhan
Duangporn Kantachote
Rattanaruji Pomwised
Monwadee Wonglapsuwan
Komwit Surachat
Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater
Life
purple non-sulfur bacteria
<i>Rhodopseudomonas</i>
wastewater treatment
genome
bioinformatics
title Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater
title_full Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater
title_fullStr Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater
title_full_unstemmed Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater
title_short Genomic Insights and Comparative Analysis of Novel <i>Rhodopseudomonas</i> Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater
title_sort genomic insights and comparative analysis of novel i rhodopseudomonas i species a purple non sulfur bacterium isolated from latex rubber sheet wastewater
topic purple non-sulfur bacteria
<i>Rhodopseudomonas</i>
wastewater treatment
genome
bioinformatics
url https://www.mdpi.com/2075-1729/15/5/754
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