Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma
Tobacco smoke contains toxic chemical substances that mediate the generation of reactive oxygen species and lung cancer. This study investigates the potential of essential oils (EOs) from Juniperus oxycedrus (JOX) and Juniperus phoenicea L. (JPH) in reducing the harmful effects of cigarette smoke. T...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/bri/6238789 |
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| author | Haouaouchi Fatma Zohra Boudiba Sameh Boudiba Louiza Baya Berka Karima Hanini Gasmi Salim Soraya Hioun Alfred Ngenge Tamfu |
| author_facet | Haouaouchi Fatma Zohra Boudiba Sameh Boudiba Louiza Baya Berka Karima Hanini Gasmi Salim Soraya Hioun Alfred Ngenge Tamfu |
| author_sort | Haouaouchi Fatma Zohra |
| collection | DOAJ |
| description | Tobacco smoke contains toxic chemical substances that mediate the generation of reactive oxygen species and lung cancer. This study investigates the potential of essential oils (EOs) from Juniperus oxycedrus (JOX) and Juniperus phoenicea L. (JPH) in reducing the harmful effects of cigarette smoke. The EO of JOX (JOX-EO) and JPH (JPH-EO) showed good inhibition of nicotine, with IC50 values of 16.78 ± 1.04 μg/mL and 18.40 ± 0.46 μg/mL, respectively. Moreover, JOX-EO had the greatest percentage reduction of basic tar (64.45%), followed by neutral tar (53.23%), and acidic tar (25.15%). Furthermore, the evaluated JOX-EO demonstrated efficacy in inhibiting polycyclic aromatic hydrocarbons (PAHs). In vivo experiments on rats chronically exposed to cigarette smoke reveal promising results regarding oxidative stress markers. The administration of JOX-EO at 200 mg/kg for 15 days postsmoking cessation shows significant improvements in hematological variables (red blood cells [RBC], platelets [PLT], hemoglobin [HB], hematocrit [HCT], and mean corpuscular volume [MCV]). The oxidative stress markers, including glutathione (GSH), malondialdehyde (MDA), glutathione S-transferase (GST), glutathione peroxidase (GPx), and catalase (CAT), were significantly reduced, indicating powerful antioxidant potential. The EOs exhibited concentration-dependent percentage inhibition of human lung carcinoma (A549) cell viability, with IC50 values indicating greater potential than etoposide, the standard used for comparison. Both EOs demonstrate the capacity to mitigate the lingering effects of smoking, such as oxidative stress and lung cancer, providing reassurance and comfort to those affected. |
| format | Article |
| id | doaj-art-c4e55fd28a63437487a57b827ad80ec6 |
| institution | Kabale University |
| issn | 2090-2255 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-c4e55fd28a63437487a57b827ad80ec62025-08-25T00:00:02ZengWileyBiochemistry Research International2090-22552025-01-01202510.1155/bri/6238789Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung AdenocarcinomaHaouaouchi Fatma Zohra0Boudiba Sameh1Boudiba Louiza2Baya Berka3Karima Hanini4Gasmi Salim5Soraya Hioun6Alfred Ngenge Tamfu7Laboratory of Organic Materials and Heterochemistry (LOMH)Laboratory of Applied Chemistry and Renewable Energies (LACRE)Laboratory of Applied Chemistry and Renewable Energies (LACRE)Animal Biology and Physiology Research Laboratory (LBPA)Laboratory of Applied Chemistry and Renewable Energies (LACRE)Laboratory of Applied Chemistry and Renewable Energies (LACRE)Laboratory of Applied Chemistry and Renewable Energies (LACRE)Department of Chemical EngineeringTobacco smoke contains toxic chemical substances that mediate the generation of reactive oxygen species and lung cancer. This study investigates the potential of essential oils (EOs) from Juniperus oxycedrus (JOX) and Juniperus phoenicea L. (JPH) in reducing the harmful effects of cigarette smoke. The EO of JOX (JOX-EO) and JPH (JPH-EO) showed good inhibition of nicotine, with IC50 values of 16.78 ± 1.04 μg/mL and 18.40 ± 0.46 μg/mL, respectively. Moreover, JOX-EO had the greatest percentage reduction of basic tar (64.45%), followed by neutral tar (53.23%), and acidic tar (25.15%). Furthermore, the evaluated JOX-EO demonstrated efficacy in inhibiting polycyclic aromatic hydrocarbons (PAHs). In vivo experiments on rats chronically exposed to cigarette smoke reveal promising results regarding oxidative stress markers. The administration of JOX-EO at 200 mg/kg for 15 days postsmoking cessation shows significant improvements in hematological variables (red blood cells [RBC], platelets [PLT], hemoglobin [HB], hematocrit [HCT], and mean corpuscular volume [MCV]). The oxidative stress markers, including glutathione (GSH), malondialdehyde (MDA), glutathione S-transferase (GST), glutathione peroxidase (GPx), and catalase (CAT), were significantly reduced, indicating powerful antioxidant potential. The EOs exhibited concentration-dependent percentage inhibition of human lung carcinoma (A549) cell viability, with IC50 values indicating greater potential than etoposide, the standard used for comparison. Both EOs demonstrate the capacity to mitigate the lingering effects of smoking, such as oxidative stress and lung cancer, providing reassurance and comfort to those affected.http://dx.doi.org/10.1155/bri/6238789 |
| spellingShingle | Haouaouchi Fatma Zohra Boudiba Sameh Boudiba Louiza Baya Berka Karima Hanini Gasmi Salim Soraya Hioun Alfred Ngenge Tamfu Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma Biochemistry Research International |
| title | Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma |
| title_full | Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma |
| title_fullStr | Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma |
| title_full_unstemmed | Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma |
| title_short | Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma |
| title_sort | effectiveness of juniper essential oils in reducing selected cigarette smoke toxicants improving oxidative parameters and cytotoxicity against lung adenocarcinoma |
| url | http://dx.doi.org/10.1155/bri/6238789 |
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