Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences
ObjectiveTo investigate the impact of anesthesia and surgery on hippocampal expression of Alzheimer’s disease (AD)-associated proteins in 5×FAD transgenic mice and explore potential sex differences. Methods5×FAD mice were crossbred with C57BL/6J wild-type (WT) mice to generate offspring for genotypi...
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Shanghai Chinese Clinical Medicine Press Co., Ltd.
2025-06-01
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| Series: | Zhongguo Linchuang Yixue |
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| Online Access: | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241401 |
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| author | Yinglin ZHANG Yong HUANG Li ZHANG Chao LIANG |
| author_facet | Yinglin ZHANG Yong HUANG Li ZHANG Chao LIANG |
| author_sort | Yinglin ZHANG |
| collection | DOAJ |
| description | ObjectiveTo investigate the impact of anesthesia and surgery on hippocampal expression of Alzheimer’s disease (AD)-associated proteins in 5×FAD transgenic mice and explore potential sex differences. Methods5×FAD mice were crossbred with C57BL/6J wild-type (WT) mice to generate offspring for genotypic confirmation. Four-month-old 5×FAD mice and littermate (LM) WT controls were allocated into 8 experimental groups (n＝8/group): female/male 5×FAD control group, female/male 5×FAD anesthesia/surgery group, female/male LM control group, and female/male LM anesthesia/surgery group. Anesthesia/surgery groups underwent laparotomy under 1.4% isoflurane anesthesia, while control groups received no intervention. Hippocampal tissues were collected 24 hours post-procedure for Western blotting analysis of β-catenin, glycogen synthase kinase 3 beta (GSK3β), and phosphorylated GSK3β (p-GSK3β) levels. ResultsFemale 5×FAD mice demonstrated significant reductions in β-catenin levels and p-GSK3β expression compared to both sex-matched LM controls and male 5×FAD counterparts (P＜0.05). No significant differences in these proteins were observed in male 5×FAD mice following anesthesia/surgery. ConclusionsThese findings reveal sex-specific responses to perioperative stress in AD, suggesting that anesthesia and surgery may affect female AD patients through hippocampal β-catenin/GSK3β pathway modulation. |
| format | Article |
| id | doaj-art-c4e19bf78c9e44f097821534cc7976e2 |
| institution | Kabale University |
| issn | 1008-6358 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Shanghai Chinese Clinical Medicine Press Co., Ltd. |
| record_format | Article |
| series | Zhongguo Linchuang Yixue |
| spelling | doaj-art-c4e19bf78c9e44f097821534cc7976e22025-08-20T03:28:51ZengShanghai Chinese Clinical Medicine Press Co., Ltd.Zhongguo Linchuang Yixue1008-63582025-06-0132349349910.12025/j.issn.1008-6358.2025.2024140120241401Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differencesYinglin ZHANG0Yong HUANG1Li ZHANG2Chao LIANG3Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaObjectiveTo investigate the impact of anesthesia and surgery on hippocampal expression of Alzheimer’s disease (AD)-associated proteins in 5×FAD transgenic mice and explore potential sex differences. Methods5×FAD mice were crossbred with C57BL/6J wild-type (WT) mice to generate offspring for genotypic confirmation. Four-month-old 5×FAD mice and littermate (LM) WT controls were allocated into 8 experimental groups (n＝8/group): female/male 5×FAD control group, female/male 5×FAD anesthesia/surgery group, female/male LM control group, and female/male LM anesthesia/surgery group. Anesthesia/surgery groups underwent laparotomy under 1.4% isoflurane anesthesia, while control groups received no intervention. Hippocampal tissues were collected 24 hours post-procedure for Western blotting analysis of β-catenin, glycogen synthase kinase 3 beta (GSK3β), and phosphorylated GSK3β (p-GSK3β) levels. ResultsFemale 5×FAD mice demonstrated significant reductions in β-catenin levels and p-GSK3β expression compared to both sex-matched LM controls and male 5×FAD counterparts (P＜0.05). No significant differences in these proteins were observed in male 5×FAD mice following anesthesia/surgery. ConclusionsThese findings reveal sex-specific responses to perioperative stress in AD, suggesting that anesthesia and surgery may affect female AD patients through hippocampal β-catenin/GSK3β pathway modulation.https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241401alzheimer’s diseaseanesthesiasurgery5×fad transgenic micesex differencesβ-catenin/gsk3β |
| spellingShingle | Yinglin ZHANG Yong HUANG Li ZHANG Chao LIANG Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences Zhongguo Linchuang Yixue alzheimer’s disease anesthesia surgery 5×fad transgenic mice sex differences β-catenin/gsk3β |
| title | Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences |
| title_full | Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences |
| title_fullStr | Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences |
| title_full_unstemmed | Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences |
| title_short | Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences |
| title_sort | effects of anesthesia and surgery on the expression of alzheimer s disease related proteins in the hippocampus of 5 fad mice and its sex differences |
| topic | alzheimer’s disease anesthesia surgery 5×fad transgenic mice sex differences β-catenin/gsk3β |
| url | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241401 |
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