An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies
Aminophylline (AMP), a xanthine-derived bronchodilator with a narrow therapeutic window, requires timely plasma concentration monitoring and pharmacokinetic (PK) studies for safe medication. However, efficient and sensitive detection methods remain limited. In this study, high quantum yield carbon d...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425005897 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849433720970280960 |
|---|---|
| author | Chenfang Miao Menghan Zhang Ying Zheng Xiaoyan Lin Shuangying Yang Linlin Xu Sitong Lu Zhengjun Huang Jianyong Huang Yanjie Zheng Shaohuang Weng |
| author_facet | Chenfang Miao Menghan Zhang Ying Zheng Xiaoyan Lin Shuangying Yang Linlin Xu Sitong Lu Zhengjun Huang Jianyong Huang Yanjie Zheng Shaohuang Weng |
| author_sort | Chenfang Miao |
| collection | DOAJ |
| description | Aminophylline (AMP), a xanthine-derived bronchodilator with a narrow therapeutic window, requires timely plasma concentration monitoring and pharmacokinetic (PK) studies for safe medication. However, efficient and sensitive detection methods remain limited. In this study, high quantum yield carbon dots (CA/EDA-CDs) were synthesized via a hydrothermal method using riboflavin as the carbon source, citric acid and ethylenediamine as functionalizing agents and dopants. The luminescent structure of CA/EDA-CDs, featuring a π-conjugated system (aromatic C=C and graphite N) and surface chromophores (C=N/C=O) connected via C-N, C-O, and hydrogen bonds, was controllably modulated by hydroxyl radical (·OH). Upon ·OH exposure, the emission band gaps of CA/EDA-CDs at 3.52 eV and 5.21 eV disappeared, while a new level at 2.63 eV emerged, leading to a quantitative and selective fluorescence quenching of CA/EDA-CDs to ·OH. Leveraging xanthine oxidase (XOD)-mediated AMP oxidation to hydrogen peroxide (H2O2), which subsequently reacted with Fe2+ to generate ·OH, a sensitive and accurate fluorescence method using CA/EDA-CDs as probe was fabricated for monitoring AMP with excellent performance. Furthermore, the developed fluorescence strategy was controllably applied to PK studies in rats administered intravenous AMP (11.25 mg/kg and 22.50 mg/kg), yielding parameters consistent with clinical data. These findings underscore the potential of CA/EDA-CDs for rapid and accurate AMP detection, addressing the clinical need for a simple and rapid monitoring method. |
| format | Article |
| id | doaj-art-c4e0aad23d684f33b5eecec7e4d47876 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-c4e0aad23d684f33b5eecec7e4d478762025-08-20T03:26:56ZengElsevierMaterials Today Bio2590-00642025-08-013310201910.1016/j.mtbio.2025.102019An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studiesChenfang Miao0Menghan Zhang1Ying Zheng2Xiaoyan Lin3Shuangying Yang4Linlin Xu5Sitong Lu6Zhengjun Huang7Jianyong Huang8Yanjie Zheng9Shaohuang Weng10Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China; The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmacy, Fujian Medical University Union Hospital, Fuzhou, 350001, China; Corresponding author.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China; The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350122, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China; The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350122, China; Corresponding author. Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China.Aminophylline (AMP), a xanthine-derived bronchodilator with a narrow therapeutic window, requires timely plasma concentration monitoring and pharmacokinetic (PK) studies for safe medication. However, efficient and sensitive detection methods remain limited. In this study, high quantum yield carbon dots (CA/EDA-CDs) were synthesized via a hydrothermal method using riboflavin as the carbon source, citric acid and ethylenediamine as functionalizing agents and dopants. The luminescent structure of CA/EDA-CDs, featuring a π-conjugated system (aromatic C=C and graphite N) and surface chromophores (C=N/C=O) connected via C-N, C-O, and hydrogen bonds, was controllably modulated by hydroxyl radical (·OH). Upon ·OH exposure, the emission band gaps of CA/EDA-CDs at 3.52 eV and 5.21 eV disappeared, while a new level at 2.63 eV emerged, leading to a quantitative and selective fluorescence quenching of CA/EDA-CDs to ·OH. Leveraging xanthine oxidase (XOD)-mediated AMP oxidation to hydrogen peroxide (H2O2), which subsequently reacted with Fe2+ to generate ·OH, a sensitive and accurate fluorescence method using CA/EDA-CDs as probe was fabricated for monitoring AMP with excellent performance. Furthermore, the developed fluorescence strategy was controllably applied to PK studies in rats administered intravenous AMP (11.25 mg/kg and 22.50 mg/kg), yielding parameters consistent with clinical data. These findings underscore the potential of CA/EDA-CDs for rapid and accurate AMP detection, addressing the clinical need for a simple and rapid monitoring method.http://www.sciencedirect.com/science/article/pii/S2590006425005897Carbon dotsHydroxyl radicalSpecific responseFluorescence strategyPharmacokinetic studyAminophylline |
| spellingShingle | Chenfang Miao Menghan Zhang Ying Zheng Xiaoyan Lin Shuangying Yang Linlin Xu Sitong Lu Zhengjun Huang Jianyong Huang Yanjie Zheng Shaohuang Weng An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies Materials Today Bio Carbon dots Hydroxyl radical Specific response Fluorescence strategy Pharmacokinetic study Aminophylline |
| title | An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies |
| title_full | An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies |
| title_fullStr | An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies |
| title_full_unstemmed | An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies |
| title_short | An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies |
| title_sort | effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots specific response to ·oh for aminophylline pharmacokinetic studies |
| topic | Carbon dots Hydroxyl radical Specific response Fluorescence strategy Pharmacokinetic study Aminophylline |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425005897 |
| work_keys_str_mv | AT chenfangmiao aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT menghanzhang aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT yingzheng aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT xiaoyanlin aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT shuangyingyang aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT linlinxu aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT sitonglu aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT zhengjunhuang aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT jianyonghuang aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT yanjiezheng aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT shaohuangweng aneffectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT chenfangmiao effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT menghanzhang effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT yingzheng effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT xiaoyanlin effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT shuangyingyang effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT linlinxu effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT sitonglu effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT zhengjunhuang effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT jianyonghuang effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT yanjiezheng effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies AT shaohuangweng effectivefluorescencestrategybasedonxanthineoxidasecatalysisandthecarbondotsspecificresponsetoohforaminophyllinepharmacokineticstudies |