Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity
Embryonic Stem (ES) or induced Pluripotent Stem (iPS) cells are important sources for cardiomyocyte generation, targeted for regenerative therapies. Several in vitro protocols are currently utilized for their differentiation, but the value of cell-based approaches remains unclear. Here, we character...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8305624 |
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| author | Violetta A. Maltabe Eleonora Barka Marianthi Kontonika Dimitra Florou Maria Kouvara-Pritsouli Maria Roumpi Simeon Agathopoulos Theofilos M. Kolettis Panos Kouklis |
| author_facet | Violetta A. Maltabe Eleonora Barka Marianthi Kontonika Dimitra Florou Maria Kouvara-Pritsouli Maria Roumpi Simeon Agathopoulos Theofilos M. Kolettis Panos Kouklis |
| author_sort | Violetta A. Maltabe |
| collection | DOAJ |
| description | Embryonic Stem (ES) or induced Pluripotent Stem (iPS) cells are important sources for cardiomyocyte generation, targeted for regenerative therapies. Several in vitro protocols are currently utilized for their differentiation, but the value of cell-based approaches remains unclear. Here, we characterized a cardiovascular progenitor population derived during ES differentiation, after selection based on VE-cadherin promoter (Pvec) activity. ESCs were genetically modified with an episomal vector, allowing the expression of puromycin resistance gene, under Pvec activity. Puromycin-surviving cells displayed cardiac and endothelial progenitor cells characteristics. Expansion and self-renewal of this cardiac and endothelial dual-progenitor population (CEDP) were achieved by Wnt/β-catenin pathway activation. CEDPs express early cardiac developmental stage-specific markers but not markers of differentiated cardiomyocytes. Similarly, CEDPs express endothelial markers. However, CEDPs can undergo differentiation predominantly to cTnT+ (~47%) and VE-cadherin+ (~28%) cells. Transplantation of CEDPs in the left heart ventricle of adult rats showed that CEDPs-derived cells survive and differentiate in vivo for at least 14 days after transplantation. A novel, dual-progenitor population was isolated during ESCs differentiation, based on Pvec activity. This lineage can self-renew, permitting its maintenance as a source of cardiovascular progenitor cells and constitutes a useful source for regenerative approaches. |
| format | Article |
| id | doaj-art-c4dfa044939b406f9f9e615afaf7482c |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c4dfa044939b406f9f9e615afaf7482c2025-08-20T02:18:47ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/83056248305624Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter ActivityVioletta A. Maltabe0Eleonora Barka1Marianthi Kontonika2Dimitra Florou3Maria Kouvara-Pritsouli4Maria Roumpi5Simeon Agathopoulos6Theofilos M. Kolettis7Panos Kouklis8Laboratory of Biology, Medical School, University of Ioannina, Ioannina, GreeceCeramics and Composites Laboratory Department of Materials Science and Engineering, University of Ioannina, Ioannina, GreeceDepartment of Cardiology, University of Ioannina Medical School, Ioannina, GreeceLaboratory of Biology, Medical School, University of Ioannina, Ioannina, GreeceLaboratory of Biology, Medical School, University of Ioannina, Ioannina, GreeceCeramics and Composites Laboratory Department of Materials Science and Engineering, University of Ioannina, Ioannina, GreeceCeramics and Composites Laboratory Department of Materials Science and Engineering, University of Ioannina, Ioannina, GreeceDepartment of Cardiology, University of Ioannina Medical School, Ioannina, GreeceLaboratory of Biology, Medical School, University of Ioannina, Ioannina, GreeceEmbryonic Stem (ES) or induced Pluripotent Stem (iPS) cells are important sources for cardiomyocyte generation, targeted for regenerative therapies. Several in vitro protocols are currently utilized for their differentiation, but the value of cell-based approaches remains unclear. Here, we characterized a cardiovascular progenitor population derived during ES differentiation, after selection based on VE-cadherin promoter (Pvec) activity. ESCs were genetically modified with an episomal vector, allowing the expression of puromycin resistance gene, under Pvec activity. Puromycin-surviving cells displayed cardiac and endothelial progenitor cells characteristics. Expansion and self-renewal of this cardiac and endothelial dual-progenitor population (CEDP) were achieved by Wnt/β-catenin pathway activation. CEDPs express early cardiac developmental stage-specific markers but not markers of differentiated cardiomyocytes. Similarly, CEDPs express endothelial markers. However, CEDPs can undergo differentiation predominantly to cTnT+ (~47%) and VE-cadherin+ (~28%) cells. Transplantation of CEDPs in the left heart ventricle of adult rats showed that CEDPs-derived cells survive and differentiate in vivo for at least 14 days after transplantation. A novel, dual-progenitor population was isolated during ESCs differentiation, based on Pvec activity. This lineage can self-renew, permitting its maintenance as a source of cardiovascular progenitor cells and constitutes a useful source for regenerative approaches.http://dx.doi.org/10.1155/2016/8305624 |
| spellingShingle | Violetta A. Maltabe Eleonora Barka Marianthi Kontonika Dimitra Florou Maria Kouvara-Pritsouli Maria Roumpi Simeon Agathopoulos Theofilos M. Kolettis Panos Kouklis Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity Stem Cells International |
| title | Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity |
| title_full | Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity |
| title_fullStr | Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity |
| title_full_unstemmed | Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity |
| title_short | Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity |
| title_sort | isolation of an es derived cardiovascular multipotent cell population based on ve cadherin promoter activity |
| url | http://dx.doi.org/10.1155/2016/8305624 |
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