Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis
Wings are an important flight organ of insects. Wing development is a complex process controlled by a series of genes. The flightless wing pad transforms into a mature wing with the function of migratory flight during the nymph-to-adult metamorphosis. However, the mechanism of wing morphogenesis in...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2024-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923003647 |
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| author | Jing Zhang Zhaochen Wu Shuo Li He Huang Suning Liu Weimin Liu Xiaoming Zhao Jianzhen Zhang |
| author_facet | Jing Zhang Zhaochen Wu Shuo Li He Huang Suning Liu Weimin Liu Xiaoming Zhao Jianzhen Zhang |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Wings are an important flight organ of insects. Wing development is a complex process controlled by a series of genes. The flightless wing pad transforms into a mature wing with the function of migratory flight during the nymph-to-adult metamorphosis. However, the mechanism of wing morphogenesis in locusts is still unclear. This study analyzed the microstructures of the locust wing pads at pre-eclosion and the wings after eclosion and performed the comparative transcriptome analysis. RNA-seq identified 25,334 unigenes and 3,430 differentially expressed genes (DEGs) (1,907 up-regulated and 1,523 down-regulated). The DEGs mainly included cuticle development (LmACPs), chitin metabolism (LmIdgf4), lipid metabolism-related genes, cell adhesion (Integrin), zinc finger transcription factors (LmSalm, LmZF593 and LmZF521), and others. Functional analysis based on RNA interference and hematoxylin and eosin (H&E) staining showed that the three genes encoded zinc finger transcription factors are essential for forming wing cuticle and maintaining morphology in Locusta migratoria. Finally, the study found that the LmSalm regulates the expression of LmACPs in the wing pads at pre-eclosion, and LmZF593 and LmZF521 regulate the expression of LmIntegrin/LmIdgf4/LmHMT420 in the wings after eclosion. This study revealed that the molecular regulatory axis controls wing morphology in nymphal and adult stages of locusts, offering a theoretical basis for the study of wing development mechanisms in hemimetabolous insects. |
| format | Article |
| id | doaj-art-c4df8d61209b4e44a08df2654f7f3e99 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-c4df8d61209b4e44a08df2654f7f3e992025-08-20T03:58:54ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192024-04-012341285129910.1016/j.jia.2023.10.022Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosisJing Zhang0Zhaochen Wu1Shuo Li2He Huang3Suning Liu4Weimin Liu5Xiaoming Zhao6Jianzhen Zhang7Shanxi Key Laboratory of Nucleic Acid Biopesticides, Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; College of Life Science, Shanxi University, Taiyuan 030006, ChinaShanxi Key Laboratory of Nucleic Acid Biopesticides, Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; College of Life Science, Shanxi University, Taiyuan 030006, ChinaCollege of Life Science, Shanxi University, Taiyuan 030006, ChinaCollege of Life Science, Shanxi University, Taiyuan 030006, ChinaGuangmeiyuan R&D Center/Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, ChinaShanxi Key Laboratory of Nucleic Acid Biopesticides, Institute of Applied Biology, Shanxi University, Taiyuan 030006, ChinaShanxi Key Laboratory of Nucleic Acid Biopesticides, Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; Correspondence Xiaoming Zhao, Jianzhen ZhangShanxi Key Laboratory of Nucleic Acid Biopesticides, Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; Correspondence Xiaoming Zhao, Jianzhen ZhangWings are an important flight organ of insects. Wing development is a complex process controlled by a series of genes. The flightless wing pad transforms into a mature wing with the function of migratory flight during the nymph-to-adult metamorphosis. However, the mechanism of wing morphogenesis in locusts is still unclear. This study analyzed the microstructures of the locust wing pads at pre-eclosion and the wings after eclosion and performed the comparative transcriptome analysis. RNA-seq identified 25,334 unigenes and 3,430 differentially expressed genes (DEGs) (1,907 up-regulated and 1,523 down-regulated). The DEGs mainly included cuticle development (LmACPs), chitin metabolism (LmIdgf4), lipid metabolism-related genes, cell adhesion (Integrin), zinc finger transcription factors (LmSalm, LmZF593 and LmZF521), and others. Functional analysis based on RNA interference and hematoxylin and eosin (H&E) staining showed that the three genes encoded zinc finger transcription factors are essential for forming wing cuticle and maintaining morphology in Locusta migratoria. Finally, the study found that the LmSalm regulates the expression of LmACPs in the wing pads at pre-eclosion, and LmZF593 and LmZF521 regulate the expression of LmIntegrin/LmIdgf4/LmHMT420 in the wings after eclosion. This study revealed that the molecular regulatory axis controls wing morphology in nymphal and adult stages of locusts, offering a theoretical basis for the study of wing development mechanisms in hemimetabolous insects.http://www.sciencedirect.com/science/article/pii/S2095311923003647Locusta migratoriawing developmentmetamorphosisRNA-seq |
| spellingShingle | Jing Zhang Zhaochen Wu Shuo Li He Huang Suning Liu Weimin Liu Xiaoming Zhao Jianzhen Zhang Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis Journal of Integrative Agriculture Locusta migratoria wing development metamorphosis RNA-seq |
| title | Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis |
| title_full | Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis |
| title_fullStr | Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis |
| title_full_unstemmed | Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis |
| title_short | Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis |
| title_sort | development and formation of wing cuticle based on transcriptomic analysis in locusta migratoria during metamorphosis |
| topic | Locusta migratoria wing development metamorphosis RNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923003647 |
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