Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter
Several empirical studies have linked subway and ambient particle exposure to toxicity, pro-inflammatory responses, and vascular dysfunction. However, the health effects of pollutants generated from varying sources, particularly when combined with lipopolysaccharide (LPS), are still unexplored. Ther...
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Elsevier
2024-12-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324014325 |
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| author | Oluwatoyin Hannah Owokoniran Akiko Honda Takamichi Ichinose Raga Ishikawa Megumi Nagao Natsuko Miyasaka Zaoshi Wang Satsuki Takai Issei Omori Kerui Zhang Wei Liu Yuya Higaki Takayuki Kameda Tomonari Matsuda Taku Fujiwara Tomoaki Okuda Hirohisa Takano |
| author_facet | Oluwatoyin Hannah Owokoniran Akiko Honda Takamichi Ichinose Raga Ishikawa Megumi Nagao Natsuko Miyasaka Zaoshi Wang Satsuki Takai Issei Omori Kerui Zhang Wei Liu Yuya Higaki Takayuki Kameda Tomonari Matsuda Taku Fujiwara Tomoaki Okuda Hirohisa Takano |
| author_sort | Oluwatoyin Hannah Owokoniran |
| collection | DOAJ |
| description | Several empirical studies have linked subway and ambient particle exposure to toxicity, pro-inflammatory responses, and vascular dysfunction. However, the health effects of pollutants generated from varying sources, particularly when combined with lipopolysaccharide (LPS), are still unexplored. Therefore, the aim of this study was to investigate the characteristic health effects of iron oxide particles (the main components of subway particles) in comparison with urban aerosols (UA) and vehicle exhaust particles (VEP), alone and in combination with LPS. This study revealed that iron oxides caused a more significant reduction in human umbilical vein endothelial cell viability, increased lactate dehydrogenase release, and decreased the production of plasminogen activator inhibitor-1, a fibrinolytic modulator, and endothelin-1, a vasoconstrictor, compared to those by VEP and UA at marginally toxic and toxic concentrations. While VEP and UA induced an increase in interleukin (IL)-6 production, iron oxides, particularly Fe3O4, increased IL-8 production at slightly toxic and non-cytotoxic concentrations. In addition, co-exposure of all particles and LPS at non-cytotoxic concentrations promoted pro-inflammatory cytokine (IL-6 and IL-8) production relative to exposure to the particles alone. Interestingly, the tendency towards either coagulation or fibrinolytic conditions was dependent on the concentration of exposed particles at the same LPS concentration. Furthermore, increases in inflammation, neutrophil and lymphocyte recruitment around blood vessels, and edema were observed in murine lungs exposed to a combination of iron oxides and LPS compared to those in mice exposed to iron oxide alone. Thus, iron oxide-rich subway particulate poses more health risks than outdoor ambient particles since they can significantly impair endothelial function, particularly through gross cellular and vascular homeostatic protein damage, and induce exacerbated inflammatory responses during co-exposure. These findings provide novel empirical evidence for epidemiological studies seeking mechanisms responsible for the observed health impact of transport- and occupational-related exposures on vascular dysfunction. |
| format | Article |
| id | doaj-art-c4cb1bf74ab74bbbb294af42a7107516 |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-c4cb1bf74ab74bbbb294af42a71075162025-08-20T02:37:28ZengElsevierEcotoxicology and Environmental Safety0147-65132024-12-0128811735610.1016/j.ecoenv.2024.117356Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matterOluwatoyin Hannah Owokoniran0Akiko Honda1Takamichi Ichinose2Raga Ishikawa3Megumi Nagao4Natsuko Miyasaka5Zaoshi Wang6Satsuki Takai7Issei Omori8Kerui Zhang9Wei Liu10Yuya Higaki11Takayuki Kameda12Tomonari Matsuda13Taku Fujiwara14Tomoaki Okuda15Hirohisa Takano16Environmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, Japan; Correspondence to: Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, C Cluster, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan.Environmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanDepartment of Socio-Environmental Energy Science, Graduate School of Energy Science, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Engineering, Kyoto University, Kyoto, JapanEnvironmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, JapanDepartment of Applied Chemistry, Faculty of Science and Technology, Keio University, Kanagawa, JapanEnvironmental Health Division, Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan; Institute for International Academic Research, Kyoto University of Advanced Science, Kyoto, Japan; Research Institute for Coexistence and Health Science, Kyoto University of Advanced Science, Kyoto, JapanSeveral empirical studies have linked subway and ambient particle exposure to toxicity, pro-inflammatory responses, and vascular dysfunction. However, the health effects of pollutants generated from varying sources, particularly when combined with lipopolysaccharide (LPS), are still unexplored. Therefore, the aim of this study was to investigate the characteristic health effects of iron oxide particles (the main components of subway particles) in comparison with urban aerosols (UA) and vehicle exhaust particles (VEP), alone and in combination with LPS. This study revealed that iron oxides caused a more significant reduction in human umbilical vein endothelial cell viability, increased lactate dehydrogenase release, and decreased the production of plasminogen activator inhibitor-1, a fibrinolytic modulator, and endothelin-1, a vasoconstrictor, compared to those by VEP and UA at marginally toxic and toxic concentrations. While VEP and UA induced an increase in interleukin (IL)-6 production, iron oxides, particularly Fe3O4, increased IL-8 production at slightly toxic and non-cytotoxic concentrations. In addition, co-exposure of all particles and LPS at non-cytotoxic concentrations promoted pro-inflammatory cytokine (IL-6 and IL-8) production relative to exposure to the particles alone. Interestingly, the tendency towards either coagulation or fibrinolytic conditions was dependent on the concentration of exposed particles at the same LPS concentration. Furthermore, increases in inflammation, neutrophil and lymphocyte recruitment around blood vessels, and edema were observed in murine lungs exposed to a combination of iron oxides and LPS compared to those in mice exposed to iron oxide alone. Thus, iron oxide-rich subway particulate poses more health risks than outdoor ambient particles since they can significantly impair endothelial function, particularly through gross cellular and vascular homeostatic protein damage, and induce exacerbated inflammatory responses during co-exposure. These findings provide novel empirical evidence for epidemiological studies seeking mechanisms responsible for the observed health impact of transport- and occupational-related exposures on vascular dysfunction.http://www.sciencedirect.com/science/article/pii/S0147651324014325Lipopolysaccharideparticulate matter exposurevascular dysfunctioninflammation inductioninnate immune responseenvironmental pollutants |
| spellingShingle | Oluwatoyin Hannah Owokoniran Akiko Honda Takamichi Ichinose Raga Ishikawa Megumi Nagao Natsuko Miyasaka Zaoshi Wang Satsuki Takai Issei Omori Kerui Zhang Wei Liu Yuya Higaki Takayuki Kameda Tomonari Matsuda Taku Fujiwara Tomoaki Okuda Hirohisa Takano Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter Ecotoxicology and Environmental Safety Lipopolysaccharide particulate matter exposure vascular dysfunction inflammation induction innate immune response environmental pollutants |
| title | Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter |
| title_full | Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter |
| title_fullStr | Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter |
| title_full_unstemmed | Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter |
| title_short | Co-exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function: A comparative study with ambient particulate matter |
| title_sort | co exposure of ferruginous components of subway particles with lipopolysaccharide impairs vascular function a comparative study with ambient particulate matter |
| topic | Lipopolysaccharide particulate matter exposure vascular dysfunction inflammation induction innate immune response environmental pollutants |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324014325 |
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