IDENTIFICATION OF STREPTOCOCCUS MILLERI GROUP (SMG) BACTERIA BY BIOCHEMICAL AND MOLECULAR METHODS
Objective: The aim of this study was to differentiate the strains of Streptococcus milleri group (SMG) bacteria using molecular, biochemical and semi-automated techniques and to investigate the results of susceptibility to commonly used antibiotics in treatment. It was anticipated that the study wou...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2023-02-01
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| Series: | Sabiad |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/6D1E794DC2094407A05A104CB9501664 |
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| Summary: | Objective: The aim of this study was to differentiate the strains of Streptococcus milleri group (SMG) bacteria using molecular, biochemical and semi-automated techniques and to investigate the results of susceptibility to commonly used antibiotics in treatment. It was anticipated that the study would draw attention to some important points about SMG bacteria and to determine their epidemiological importance. Materials and method: A total of 100 streptococcal bacteria were included in our study. Biochemical tests and the API 20 STREP semiautomatic identification kit were used for species identification. Species identifications were confirmed by amplification of the target gene regions (HYL-MIX-U, HLY-INT-D, HLY-CC-D, 16S-ANG-U, 16S-ANG-D, ILY-4DFw, ILYwholeC Bw) by polymerase chain reaction (PCR). Results: Voges-Proskauer tests of all strains were positive, whereas hippurate hydrolysis and pyrrolidinyl arylamidase (PYR) tests were negative. All strains were evaluated in terms of Lancefield group antigen, and it was found that 42% were G, 26% were F, and 5% were C group antigen. It was not possible to determine antigen type with Lancefield group antigens in 27% of the strains. All of the isolates were found to be susceptible to vancomycin, ceftriaxone, cefepime, cefotaxime, levofloxacin, and linezolid. Erythromycin, clindamycin, tetracycline, and chloramphenicol resistance was found in 16%, 6%, 5%, and %1 of the isolates, respectively. Ampicillin and penicillin resistance were determined by the E-test method. One Streptococcus anginosus strain was resistant to ampicillin and penicillin, and two S. anginosus isolates were found to be intermediately susceptible to ampicillin. According to API 20 STREP results, 41% of the isolates were identified as genus level, 45% were S. anginosus, 5% were Streptococcus constellatus, and 1% were Streptococcus intermedius. Multiplex polymerase chain reaction (PCR) results were found to be 56% S. anginosus, 24% S. constellatus, and 8% S. intermedius. No band was detected in 12% of the strains. It has been determined that the biochemical identification scheme of the Streptococcus milleri group has not yet been fully defined. According to the antibiotic susceptibility results, the development of resistance in SMG is not critical. Commercial kits are unsatisfactory in identifying this group, and the studies should be supported with molecular methods. |
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| ISSN: | 2651-4060 |