The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin
Background: Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear. Purpose: This study aims to verify the effectiveness of PR...
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Elsevier
2025-03-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580824002644 |
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| author | Shi-lin Lian Jie Huang Yan Zhang Yu Ding |
| author_facet | Shi-lin Lian Jie Huang Yan Zhang Yu Ding |
| author_sort | Shi-lin Lian |
| collection | DOAJ |
| description | Background: Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear. Purpose: This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin. Methods: Primary NP cells were isolated from SD rats, and a ferroptosis model was established using Erastin. PRP was prepared and applied to assess its impact on ferroptosis-related markers, including reactive oxygen species (ROS), iron content, and glutathione peroxidase 4 (GPX4). The effects of PRP on the ultrastructure of NP cells were also observed using transmission electron microscopy (TEM). Results: PRP treatment significantly restored GPX4 levels (431.47 ± 4.70 ng/L vs. 69.70 ± 4.06 ng/L, P < 0.05), reduced ROS levels (45.06 ± 3.78 vs. 155.36 ± 3.56, P < 0.05), and decreased iron content (32.24 ± 096 μg/L vs. 59.25 ± 3.72 μg/L, P < 0.05) in ferroptotic NP cells compared to the sham group. Additionally, PRP significantly increased the expression levels of collagen Ⅱ (0.72 ± 0.02 vs. 0.33 ± 0.02, P < 0.05) and aggrecan (0.81 ± 0.01 vs. 0.31 ± 0.02, P < 0.05) compared to the sham group. TEM analysis also showed improvements in mitochondrial ultrastructure. These findings suggest that PRP can alleviate ferroptosis and promote cellular recovery. Conclusions: The study demonstrates the potential of PRP as a therapeutic intervention in IVDD by mitigating ferroptosis in NP cells, offering a new theoretical basis for PRP treatment in degenerative disc diseases. |
| format | Article |
| id | doaj-art-c4c7ac527fcf41f5a72fba59d07d6a30 |
| institution | DOAJ |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-c4c7ac527fcf41f5a72fba59d07d6a302025-08-20T03:15:16ZengElsevierBiochemistry and Biophysics Reports2405-58082025-03-014110190010.1016/j.bbrep.2024.101900The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by ErastinShi-lin Lian0Jie Huang1Yan Zhang2Yu Ding3Orthopedics of TCM Senior Department, The Sixth Medical Center of PLA General Hospital, Beijing, 100048, China; Department of Orthopedics, School of Medicine, South China University of Technology, Guangzhou, 510006, ChinaOrthopedics of TCM Senior Department, The Sixth Medical Center of PLA General Hospital, Beijing, 100048, China; Department of Orthopedics, School of Medicine, South China University of Technology, Guangzhou, 510006, ChinaOrthopedics of TCM Senior Department, The Sixth Medical Center of PLA General Hospital, Beijing, 100048, ChinaOrthopedics of TCM Senior Department, The Sixth Medical Center of PLA General Hospital, Beijing, 100048, China; Corresponding author.Background: Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear. Purpose: This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin. Methods: Primary NP cells were isolated from SD rats, and a ferroptosis model was established using Erastin. PRP was prepared and applied to assess its impact on ferroptosis-related markers, including reactive oxygen species (ROS), iron content, and glutathione peroxidase 4 (GPX4). The effects of PRP on the ultrastructure of NP cells were also observed using transmission electron microscopy (TEM). Results: PRP treatment significantly restored GPX4 levels (431.47 ± 4.70 ng/L vs. 69.70 ± 4.06 ng/L, P < 0.05), reduced ROS levels (45.06 ± 3.78 vs. 155.36 ± 3.56, P < 0.05), and decreased iron content (32.24 ± 096 μg/L vs. 59.25 ± 3.72 μg/L, P < 0.05) in ferroptotic NP cells compared to the sham group. Additionally, PRP significantly increased the expression levels of collagen Ⅱ (0.72 ± 0.02 vs. 0.33 ± 0.02, P < 0.05) and aggrecan (0.81 ± 0.01 vs. 0.31 ± 0.02, P < 0.05) compared to the sham group. TEM analysis also showed improvements in mitochondrial ultrastructure. These findings suggest that PRP can alleviate ferroptosis and promote cellular recovery. Conclusions: The study demonstrates the potential of PRP as a therapeutic intervention in IVDD by mitigating ferroptosis in NP cells, offering a new theoretical basis for PRP treatment in degenerative disc diseases.http://www.sciencedirect.com/science/article/pii/S2405580824002644Platelet-rich plasmaIntervertebral disc degenerationFerroptosisNucleus pulposus cellMitochondria |
| spellingShingle | Shi-lin Lian Jie Huang Yan Zhang Yu Ding The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin Biochemistry and Biophysics Reports Platelet-rich plasma Intervertebral disc degeneration Ferroptosis Nucleus pulposus cell Mitochondria |
| title | The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin |
| title_full | The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin |
| title_fullStr | The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin |
| title_full_unstemmed | The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin |
| title_short | The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin |
| title_sort | effect of platelet rich plasma on ferroptosis of nucleus pulposus cells induced by erastin |
| topic | Platelet-rich plasma Intervertebral disc degeneration Ferroptosis Nucleus pulposus cell Mitochondria |
| url | http://www.sciencedirect.com/science/article/pii/S2405580824002644 |
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