Bio-chemical analysis of “Datura stramonium” extract
Datura stramonium is an annual plant which belongs to the Solanaceae family. Datura stramonium is also a toxic plant, with toxicity given by the presence of alkaloids hyoscyamine, atropine and scopolamine.This study performs phytochemical and antibacterial analysis of ethanolic extracts from Datura...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cluj University Press
2017-12-01
|
| Series: | Studia Universitatis Babeș-Bolyai. Biologia |
| Subjects: | |
| Online Access: | https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/93 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849430992365813760 |
|---|---|
| author | Rahela CARPA Dana-Victoria DUMITRU Ramona Flavia BURTESCU Maria Cornelia MAIOR Cristina DOBROTĂ Neli-Kinga OLAH |
| author_facet | Rahela CARPA Dana-Victoria DUMITRU Ramona Flavia BURTESCU Maria Cornelia MAIOR Cristina DOBROTĂ Neli-Kinga OLAH |
| author_sort | Rahela CARPA |
| collection | DOAJ |
| description | Datura stramonium is an annual plant which belongs to the Solanaceae family. Datura stramonium is also a toxic plant, with toxicity given by the presence of alkaloids hyoscyamine, atropine and scopolamine.This study performs phytochemical and antibacterial analysis of ethanolic extracts from Datura stramonium. Fresh plants have been used in order to obtain Datura stramonium tincture. The quality index was 1:5, the concentration of ethanol being 90%. Based on this tincture a qualitative and quantitative phytochemical analysis was performed through thin layer chromatography and high performance liquid chromatography. By thin-layer chromatography the qualitative alkaloids, such as atropine and scopolamine from Datura stramonium extract, have been identified. By high performance liquid chromatography with reversed phase the alkaloids quantity from Datura stramonium herbs was assessed and that is 1.7 mg/mL. The plant extracts were tested on Gram negative bacteria Escherichia coli and on Gram positive bacteria Staphylococcus aureus. Both tested strains showed resistance but for E. coli a higher inhibition was observed at all samples containing Datura extract. |
| format | Article |
| id | doaj-art-c4c5eb004db14e91aa68ca361769c675 |
| institution | Kabale University |
| issn | 2065-9512 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Cluj University Press |
| record_format | Article |
| series | Studia Universitatis Babeș-Bolyai. Biologia |
| spelling | doaj-art-c4c5eb004db14e91aa68ca361769c6752025-08-20T03:27:47ZengCluj University PressStudia Universitatis Babeș-Bolyai. Biologia2065-95122017-12-0151910.24193/subbbiol.2017.2.0193Bio-chemical analysis of “Datura stramonium” extractRahela CARPA0https://orcid.org/0000-0001-8974-1718Dana-Victoria DUMITRU1Ramona Flavia BURTESCU2Maria Cornelia MAIOR3Cristina DOBROTĂ4Neli-Kinga OLAH5Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.comBabeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. Email: k_hella@yahoo.comSC Plant Extrakt SRL, Radaia, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.comBabeș-Bolyai University, Institute of Technology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.comBabeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania. E-mail: k_hella@yahoo.comVasile Goldiș Western University of Arad, Faculty of Pharmacy, Arad, Romania. E-mail: k_hella@yahoo.comDatura stramonium is an annual plant which belongs to the Solanaceae family. Datura stramonium is also a toxic plant, with toxicity given by the presence of alkaloids hyoscyamine, atropine and scopolamine.This study performs phytochemical and antibacterial analysis of ethanolic extracts from Datura stramonium. Fresh plants have been used in order to obtain Datura stramonium tincture. The quality index was 1:5, the concentration of ethanol being 90%. Based on this tincture a qualitative and quantitative phytochemical analysis was performed through thin layer chromatography and high performance liquid chromatography. By thin-layer chromatography the qualitative alkaloids, such as atropine and scopolamine from Datura stramonium extract, have been identified. By high performance liquid chromatography with reversed phase the alkaloids quantity from Datura stramonium herbs was assessed and that is 1.7 mg/mL. The plant extracts were tested on Gram negative bacteria Escherichia coli and on Gram positive bacteria Staphylococcus aureus. Both tested strains showed resistance but for E. coli a higher inhibition was observed at all samples containing Datura extract.https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/93atropine, bacterial susceptibility, datura stramonium, high performance liquid chromatography with reversed phase, scopolamine, thin-layer chromatography. |
| spellingShingle | Rahela CARPA Dana-Victoria DUMITRU Ramona Flavia BURTESCU Maria Cornelia MAIOR Cristina DOBROTĂ Neli-Kinga OLAH Bio-chemical analysis of “Datura stramonium” extract Studia Universitatis Babeș-Bolyai. Biologia atropine, bacterial susceptibility, datura stramonium, high performance liquid chromatography with reversed phase, scopolamine, thin-layer chromatography. |
| title | Bio-chemical analysis of “Datura stramonium” extract |
| title_full | Bio-chemical analysis of “Datura stramonium” extract |
| title_fullStr | Bio-chemical analysis of “Datura stramonium” extract |
| title_full_unstemmed | Bio-chemical analysis of “Datura stramonium” extract |
| title_short | Bio-chemical analysis of “Datura stramonium” extract |
| title_sort | bio chemical analysis of datura stramonium extract |
| topic | atropine, bacterial susceptibility, datura stramonium, high performance liquid chromatography with reversed phase, scopolamine, thin-layer chromatography. |
| url | https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/93 |
| work_keys_str_mv | AT rahelacarpa biochemicalanalysisofdaturastramoniumextract AT danavictoriadumitru biochemicalanalysisofdaturastramoniumextract AT ramonaflaviaburtescu biochemicalanalysisofdaturastramoniumextract AT mariacorneliamaior biochemicalanalysisofdaturastramoniumextract AT cristinadobrota biochemicalanalysisofdaturastramoniumextract AT nelikingaolah biochemicalanalysisofdaturastramoniumextract |