Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients

The aim of this study was to investigate the relationship between the combined effect of MTHFR C677T (rs1801133) and EPHX2 G860A (rs751141) polymorphism and ischemic stroke in Chinese T2DM patients. This case-control study included a total of 626 Chinese T2DM patients (236 T2DM patients with ischemi...

Full description

Saved in:
Bibliographic Details
Main Authors: Liang Ma, Yongwei Jiang, Xiaomu Kong, Meihua Yan, Tingting Zhao, Hailing Zhao, Qian Liu, Haojun Zhang, Yongtong Cao, Ping Li
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Journal of Diabetes Research
Online Access:http://dx.doi.org/10.1155/2017/6216205
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849685561573376000
author Liang Ma
Yongwei Jiang
Xiaomu Kong
Meihua Yan
Tingting Zhao
Hailing Zhao
Qian Liu
Haojun Zhang
Yongtong Cao
Ping Li
author_facet Liang Ma
Yongwei Jiang
Xiaomu Kong
Meihua Yan
Tingting Zhao
Hailing Zhao
Qian Liu
Haojun Zhang
Yongtong Cao
Ping Li
author_sort Liang Ma
collection DOAJ
description The aim of this study was to investigate the relationship between the combined effect of MTHFR C677T (rs1801133) and EPHX2 G860A (rs751141) polymorphism and ischemic stroke in Chinese T2DM patients. This case-control study included a total of 626 Chinese T2DM patients (236 T2DM patients with ischemic stroke and 390 T2DM patients without ischemic stroke). The rs1801133 and rs751141 were genotyped using real-time polymerase chain reaction. Statistical analysis was performed with SPSS 17.0. Results showed that the combined effect of MTHFR TT and EPHX2 GG or GA + AA genotype has a higher risk of ischemic stroke compared with the control group (combined effect of MTHFR CC and EPHX2 GA + AA genotypes; OR = 3.46 and OR = 3.42, resp.; P=.001 and P=.002, resp.). The A allele showed marked association with a lower risk of ischemic stroke in patients with the lowest Hcy levels under additive, recessive, and dominant genetic models (OR = 0.45, OR = 0.11, and OR = 0.44, resp.; P=.002, P=.035, and P=.008, resp.), which was not observed in medium or high Hcy level groups. In conclusion, the T allele of rs1801133 and the G allele of rs751141 may be risk factors of ischemic stroke in the Chinese T2DM population.
format Article
id doaj-art-c4c225bcb412434c9e2b2cc86a69e59d
institution DOAJ
issn 2314-6745
2314-6753
language English
publishDate 2017-01-01
publisher Wiley
record_format Article
series Journal of Diabetes Research
spelling doaj-art-c4c225bcb412434c9e2b2cc86a69e59d2025-08-20T03:23:06ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/62162056216205Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic PatientsLiang Ma0Yongwei Jiang1Xiaomu Kong2Meihua Yan3Tingting Zhao4Hailing Zhao5Qian Liu6Haojun Zhang7Yongtong Cao8Ping Li9Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaClinical Laboratory, China-Japan Friendship Hospital, Beijing, ChinaDepartment of Endocrinology, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing, ChinaClinical Laboratory, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing, ChinaClinical Laboratory, China-Japan Friendship Hospital, Beijing, ChinaGraduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaThe aim of this study was to investigate the relationship between the combined effect of MTHFR C677T (rs1801133) and EPHX2 G860A (rs751141) polymorphism and ischemic stroke in Chinese T2DM patients. This case-control study included a total of 626 Chinese T2DM patients (236 T2DM patients with ischemic stroke and 390 T2DM patients without ischemic stroke). The rs1801133 and rs751141 were genotyped using real-time polymerase chain reaction. Statistical analysis was performed with SPSS 17.0. Results showed that the combined effect of MTHFR TT and EPHX2 GG or GA + AA genotype has a higher risk of ischemic stroke compared with the control group (combined effect of MTHFR CC and EPHX2 GA + AA genotypes; OR = 3.46 and OR = 3.42, resp.; P=.001 and P=.002, resp.). The A allele showed marked association with a lower risk of ischemic stroke in patients with the lowest Hcy levels under additive, recessive, and dominant genetic models (OR = 0.45, OR = 0.11, and OR = 0.44, resp.; P=.002, P=.035, and P=.008, resp.), which was not observed in medium or high Hcy level groups. In conclusion, the T allele of rs1801133 and the G allele of rs751141 may be risk factors of ischemic stroke in the Chinese T2DM population.http://dx.doi.org/10.1155/2017/6216205
spellingShingle Liang Ma
Yongwei Jiang
Xiaomu Kong
Meihua Yan
Tingting Zhao
Hailing Zhao
Qian Liu
Haojun Zhang
Yongtong Cao
Ping Li
Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
Journal of Diabetes Research
title Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
title_full Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
title_fullStr Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
title_full_unstemmed Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
title_short Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
title_sort synergistic effect of the mthfr c677t and ephx2 g860a polymorphism on the increased risk of ischemic stroke in chinese type 2 diabetic patients
url http://dx.doi.org/10.1155/2017/6216205
work_keys_str_mv AT liangma synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT yongweijiang synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT xiaomukong synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT meihuayan synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT tingtingzhao synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT hailingzhao synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT qianliu synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT haojunzhang synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT yongtongcao synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients
AT pingli synergisticeffectofthemthfrc677tandephx2g860apolymorphismontheincreasedriskofischemicstrokeinchinesetype2diabeticpatients