DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis
Background. Tuberculosis (TB) is usually caused by Mycobacterium tuberculosis, which has the highest mortality rate among infectious diseases. This study is designed to identify the key genes affecting the diagnosis and treatment of TB. Methods. GSE54992, which included 39 peripheral blood mononucle...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Canadian Respiratory Journal |
| Online Access: | http://dx.doi.org/10.1155/2020/4348371 |
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| _version_ | 1849685559045259264 |
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| author | Yunli Zhang Yanming Li Hongling Li Qingxia Liu Wei Wang Zijuan Jian Wenen Liu |
| author_facet | Yunli Zhang Yanming Li Hongling Li Qingxia Liu Wei Wang Zijuan Jian Wenen Liu |
| author_sort | Yunli Zhang |
| collection | DOAJ |
| description | Background. Tuberculosis (TB) is usually caused by Mycobacterium tuberculosis, which has the highest mortality rate among infectious diseases. This study is designed to identify the key genes affecting the diagnosis and treatment of TB. Methods. GSE54992, which included 39 peripheral blood mononuclear cell (PBMC) samples, was extracted from the Gene Expression Omnibus database. After the samples were classified into type and time groups by limma package, the differentially expressed genes (DEGs) were analyzed using the Analysis of Variance. Using pheatmap package, hierarchical cluster analysis was performed for the DEGs. Then, the key modules correlated with TB were selected using the WGCNA package. Finally, functional and pathway enrichment analyses were carried out using clusterProfiler package. Results. The DEGs in subclusters 3, 6, 7, and 8 were chosen for further analyses. Based on WGCNA analysis, blue and green modules in type group and pink module in time group were selected as key modules. From the key modules, 9 (including BAX and ARPC1B) hub genes in type group and 6 (including DHX36) hub genes in time group were screened. Through pathway enrichment analysis, the TNF signaling pathway was enriched for the green module. Conclusion. DHX36, BAX, and ARPC1B might be key genes acting in the mechanisms of TB. Besides, the TNF signaling pathway might also be critical for the diagnosis and therapy of the disease. |
| format | Article |
| id | doaj-art-c4bb6b3f89be47c3bb60618346f1f76c |
| institution | DOAJ |
| issn | 1198-2241 1916-7245 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Respiratory Journal |
| spelling | doaj-art-c4bb6b3f89be47c3bb60618346f1f76c2025-08-20T03:23:06ZengWileyCanadian Respiratory Journal1198-22411916-72452020-01-01202010.1155/2020/43483714348371DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of TuberculosisYunli Zhang0Yanming Li1Hongling Li2Qingxia Liu3Wei Wang4Zijuan Jian5Wenen Liu6Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha 410008, Hunan, ChinaBackground. Tuberculosis (TB) is usually caused by Mycobacterium tuberculosis, which has the highest mortality rate among infectious diseases. This study is designed to identify the key genes affecting the diagnosis and treatment of TB. Methods. GSE54992, which included 39 peripheral blood mononuclear cell (PBMC) samples, was extracted from the Gene Expression Omnibus database. After the samples were classified into type and time groups by limma package, the differentially expressed genes (DEGs) were analyzed using the Analysis of Variance. Using pheatmap package, hierarchical cluster analysis was performed for the DEGs. Then, the key modules correlated with TB were selected using the WGCNA package. Finally, functional and pathway enrichment analyses were carried out using clusterProfiler package. Results. The DEGs in subclusters 3, 6, 7, and 8 were chosen for further analyses. Based on WGCNA analysis, blue and green modules in type group and pink module in time group were selected as key modules. From the key modules, 9 (including BAX and ARPC1B) hub genes in type group and 6 (including DHX36) hub genes in time group were screened. Through pathway enrichment analysis, the TNF signaling pathway was enriched for the green module. Conclusion. DHX36, BAX, and ARPC1B might be key genes acting in the mechanisms of TB. Besides, the TNF signaling pathway might also be critical for the diagnosis and therapy of the disease.http://dx.doi.org/10.1155/2020/4348371 |
| spellingShingle | Yunli Zhang Yanming Li Hongling Li Qingxia Liu Wei Wang Zijuan Jian Wenen Liu DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis Canadian Respiratory Journal |
| title | DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis |
| title_full | DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis |
| title_fullStr | DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis |
| title_full_unstemmed | DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis |
| title_short | DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis |
| title_sort | dhx36 bax and arpc1b may be critical for the diagnosis and treatment of tuberculosis |
| url | http://dx.doi.org/10.1155/2020/4348371 |
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