Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role
Connexins (Cxs) are critical for normal tissue development, differentiation, and cell proliferation. Normal expression and function of Cxs are considered to play a role in tumor suppression, but abnormal localization and abnormally increased expression of Cxs have been found in a variety of carcinom...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2011/561719 |
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| author | Yusheng Han Paul J. Zhang Terina Chen Sabrina W. Yum Teresa Pasha Emma E. Furth |
| author_facet | Yusheng Han Paul J. Zhang Terina Chen Sabrina W. Yum Teresa Pasha Emma E. Furth |
| author_sort | Yusheng Han |
| collection | DOAJ |
| description | Connexins (Cxs) are critical for normal tissue development, differentiation, and cell proliferation. Normal expression and function of Cxs are considered to play a role in tumor
suppression, but abnormal localization and abnormally increased expression of Cxs have been found in a variety of carcinomas. Of the Cx family, Cx43 is a most prevalent member and has been known as a downstream target of β-catenin, a key component of Wnt signaling pathway. We evaluated the expression of Cx43 in the colonic neoplasia progression sequence with additional attention to the stromal component. Resections of 50 colonic adenocarcinomas were stained immunohistochemically for Cx43 on paraffin embedded sections. Cx43 cytoplasmic expression increased progressively in the colonic adenocarcinoma sequence in both the epithelial [normal (4±1), adenomatous (20±2), cancerous (124±10) (P<0.01)], and stromal [normal (19±1), cancerous (45±4) (P<0.01)] components. In the epithelial component, Cx43 was expressed lower in stage I adenocarcinomas (69±12) compared to stage III/IV (158±10, P<0.01). Additionally, Cx43 was relatively increased in the adenocarcinoma at the invasive tumor front in
all stages. Cx43 may play a critical role in the pathogenesis of colon cancer via gap junction or
other gap junction independent mechanisms such as the Wnt/β-catenin pathway. |
| format | Article |
| id | doaj-art-c4b70c15ded941fe923b68fdf90de069 |
| institution | DOAJ |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-c4b70c15ded941fe923b68fdf90de0692025-08-20T03:23:06ZengWileyGastroenterology Research and Practice1687-61211687-630X2011-01-01201110.1155/2011/561719561719Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic RoleYusheng Han0Paul J. Zhang1Terina Chen2Sabrina W. Yum3Teresa Pasha4Emma E. Furth5Department of Pathology and Laboratory Medicine, Pennsylvania Hospital, Philadelphia, PA 19107, USADepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104-4280, USADepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104-4280, USADivision of Neurology, Department of Pediatrics, Children Hospital of Philadelphia, Philadelphia, PA 19104, USADepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104-4280, USADepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104-4280, USAConnexins (Cxs) are critical for normal tissue development, differentiation, and cell proliferation. Normal expression and function of Cxs are considered to play a role in tumor suppression, but abnormal localization and abnormally increased expression of Cxs have been found in a variety of carcinomas. Of the Cx family, Cx43 is a most prevalent member and has been known as a downstream target of β-catenin, a key component of Wnt signaling pathway. We evaluated the expression of Cx43 in the colonic neoplasia progression sequence with additional attention to the stromal component. Resections of 50 colonic adenocarcinomas were stained immunohistochemically for Cx43 on paraffin embedded sections. Cx43 cytoplasmic expression increased progressively in the colonic adenocarcinoma sequence in both the epithelial [normal (4±1), adenomatous (20±2), cancerous (124±10) (P<0.01)], and stromal [normal (19±1), cancerous (45±4) (P<0.01)] components. In the epithelial component, Cx43 was expressed lower in stage I adenocarcinomas (69±12) compared to stage III/IV (158±10, P<0.01). Additionally, Cx43 was relatively increased in the adenocarcinoma at the invasive tumor front in all stages. Cx43 may play a critical role in the pathogenesis of colon cancer via gap junction or other gap junction independent mechanisms such as the Wnt/β-catenin pathway.http://dx.doi.org/10.1155/2011/561719 |
| spellingShingle | Yusheng Han Paul J. Zhang Terina Chen Sabrina W. Yum Teresa Pasha Emma E. Furth Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role Gastroenterology Research and Practice |
| title | Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role |
| title_full | Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role |
| title_fullStr | Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role |
| title_full_unstemmed | Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role |
| title_short | Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role |
| title_sort | connexin43 expression increases in the epithelium and stroma along the colonic neoplastic progression pathway implications for its oncogenic role |
| url | http://dx.doi.org/10.1155/2011/561719 |
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