Ex vivo model of using the method of optical skin clearing during antimicrobial photodynamic action
This study evaluated the effi cacy of transcutaneous photodynamic therapy using blue (428 nm) LED irradiation on Staphylococcus aureus 11 in combination with a water-soluble cationic pyridyl porphyrin and optical clearing agents (OCA) in an ex vivo model. Results showed that OCA signifi cantly enhan...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Saratov State University
2025-03-01
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| Series: | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
| Subjects: | |
| Online Access: | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2025/04/himiya_2025_1-76-88.pdf |
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| Summary: | This study evaluated the effi cacy of transcutaneous photodynamic therapy using blue (428 nm) LED irradiation on Staphylococcus aureus 11 in combination with a water-soluble cationic pyridyl porphyrin and optical clearing agents (OCA) in an ex vivo model. Results showed that OCA signifi cantly enhanced photodynamic inactivation with a 61% reduction in bacterial cell counts after 15 minutes of light exposure, comparable to direct irradiation. Optical parameter analysis revealed a decrease in scattering and absorption coeffi cients and an increase in light penetration depth (up to 121,6%) in OCA-treated skin samples. The results confi rm that optical clearing improves the effi cacy of antimicrobial photodynamic action by enhancing light penetration into deeper tissue layers, reducing the need for high laser intensities, and minimizing superfi cial tissue damage. This approach holds promise for the treatment of skin, mucosal and soft tissue infections in humans and animals, off ering valuable insights into light-tissue interactions and optimizing photodynamic therapy while reducing the risks associated with the use of LEDs and lasers. |
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| ISSN: | 1816-9775 2541-8971 |