Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes
To screen endophytic strains with strong antibacterial activity from grape leaves and assess their biocontrol potential, providing a theoretical basis for the development of new biological control strategies against grape Aspergillus rot disease. Using the plate confrontation method, antagonistic st...
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The editorial department of Science and Technology of Food Industry
2025-05-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070065 |
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| author | Miaomiao ZHANG Yizhong YANG Yuchen ZHANG Jie MA Zhenzhen XU Wei WANG Lijuan ZHANG Ning WANG Yingying FAN Cheng WANG Wei HUANG Fengjuan LIU |
| author_facet | Miaomiao ZHANG Yizhong YANG Yuchen ZHANG Jie MA Zhenzhen XU Wei WANG Lijuan ZHANG Ning WANG Yingying FAN Cheng WANG Wei HUANG Fengjuan LIU |
| author_sort | Miaomiao ZHANG |
| collection | DOAJ |
| description | To screen endophytic strains with strong antibacterial activity from grape leaves and assess their biocontrol potential, providing a theoretical basis for the development of new biological control strategies against grape Aspergillus rot disease. Using the plate confrontation method, antagonistic strains displaying notable inhibitory effects on Aspergillus niger were isolated from the leaves of 'Thompson Seedless Grapes'. Morphological, physiological, biochemical, and molecular-biological analyses identified the antagonistic strains to species-level resolution. Lipopeptide extracts from the fermentation supernatant were analyzed by UPLC-Vion-IMS-QTof mass spectrometry, revealing their chemical composition. In vivo experiments further confirmed the efficacy of the antagonistic fermentation broth in reducing postharvest Aspergillus rot on 'Thompson Seedless Grapes'. From the leaves of 'Thompson Seedless Grapes', 25 endophytic bacterial strains with inhibitory effects on A. niger were isolated. Among these, strain TLF4-3 demonstrated the strongest antagonistic activity, exhibiting an inhibition zone diameter of 27.27±0.05 mm and an inhibitory titer of 18.27±0.53 cm/mL. UPLC-Vion-IMS-QTof analysis identified the primary antimicrobial compounds produced by this strain as bacillomycin D2 (C15) and iturin C (C14). Treatments with TLF4-3 fermentation broth at 3 days postharvest achieved the best control effect, reducing lesion diameter by 2.3 mm and achieving an antibacterial rate of 68.26%±0.91%. These results offer a theoretical framework and pragmatic direction for the control and avoidance of Aspergillus rot in postharvest grapes. |
| format | Article |
| id | doaj-art-c4a9e60173fe4427b872210da9d2a088 |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-c4a9e60173fe4427b872210da9d2a0882025-08-20T02:15:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-05-01461018119010.13386/j.issn1002-0306.20240700652024070065-10Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless GrapesMiaomiao ZHANG0Yizhong YANG1Yuchen ZHANG2Jie MA3Zhenzhen XU4Wei WANG5Lijuan ZHANG6Ning WANG7Yingying FAN8Cheng WANG9Wei HUANG10Fengjuan LIU11College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaInstitute of Quality Standard & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaMicrobial Application Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, ChinaMicrobial Application Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, ChinaMicrobial Application Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, ChinaKey Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaMicrobial Application Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, ChinaKey Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaTo screen endophytic strains with strong antibacterial activity from grape leaves and assess their biocontrol potential, providing a theoretical basis for the development of new biological control strategies against grape Aspergillus rot disease. Using the plate confrontation method, antagonistic strains displaying notable inhibitory effects on Aspergillus niger were isolated from the leaves of 'Thompson Seedless Grapes'. Morphological, physiological, biochemical, and molecular-biological analyses identified the antagonistic strains to species-level resolution. Lipopeptide extracts from the fermentation supernatant were analyzed by UPLC-Vion-IMS-QTof mass spectrometry, revealing their chemical composition. In vivo experiments further confirmed the efficacy of the antagonistic fermentation broth in reducing postharvest Aspergillus rot on 'Thompson Seedless Grapes'. From the leaves of 'Thompson Seedless Grapes', 25 endophytic bacterial strains with inhibitory effects on A. niger were isolated. Among these, strain TLF4-3 demonstrated the strongest antagonistic activity, exhibiting an inhibition zone diameter of 27.27±0.05 mm and an inhibitory titer of 18.27±0.53 cm/mL. UPLC-Vion-IMS-QTof analysis identified the primary antimicrobial compounds produced by this strain as bacillomycin D2 (C15) and iturin C (C14). Treatments with TLF4-3 fermentation broth at 3 days postharvest achieved the best control effect, reducing lesion diameter by 2.3 mm and achieving an antibacterial rate of 68.26%±0.91%. These results offer a theoretical framework and pragmatic direction for the control and avoidance of Aspergillus rot in postharvest grapes.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070065antagonizing endogenous bacterialipopeptidesa. nigerb. velezensisbiological control |
| spellingShingle | Miaomiao ZHANG Yizhong YANG Yuchen ZHANG Jie MA Zhenzhen XU Wei WANG Lijuan ZHANG Ning WANG Yingying FAN Cheng WANG Wei HUANG Fengjuan LIU Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes Shipin gongye ke-ji antagonizing endogenous bacteria lipopeptides a. niger b. velezensis biological control |
| title | Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes |
| title_full | Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes |
| title_fullStr | Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes |
| title_full_unstemmed | Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes |
| title_short | Identification of Endogenous Antagonistic Bacteria in Grape Leaves and Its Inhibitory Effect on Postharvest Aspergillus niger in Thompson Seedless Grapes |
| title_sort | identification of endogenous antagonistic bacteria in grape leaves and its inhibitory effect on postharvest aspergillus niger in thompson seedless grapes |
| topic | antagonizing endogenous bacteria lipopeptides a. niger b. velezensis biological control |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070065 |
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